Orexins, also referred to as Hypocretins, are produced by specialized neurons in the lateral hypothalamus, which project into many different regions of the brain. Orexin A (33 amino acids) and Orexin B (28 amino acids) are both produced from a single pre-pro-orexin polypeptide by a cascade of enzymatic reactions.
The orexin system is conserved in mammals, and plays a central role in regulating feeding, sleep/wake cycles, arousal, energy expenditure, reward seeking, cognition and stress responses. Defects in orexin signaling are involved in diet-induced obesity, diabetes, narcolepsy, panic anxiety disorder, depression and addiction.
Synaptic Systems offers 2 polyclonal Guinea pig antibodies against Orexin, on recognizing specifically Orexin A (cat. no. 389 004) and one Orexin A/B (cat. no. 389 104) which react with both forms. This antibodies display superior results in IHC and IHC-P (FFPE) applications and the Orexin A antibody is KO validated.
Indirect immunostaining of PFA fixed WT mouse brain section (hypothalamus) with Guinea pig anti-Orexin A (cat. no. 389 004, dilution 1:500 red).
Courtesy: Dana Mayer & Markus Fendt, Institute of Pharmacology and Toxicology, Magedeburg
Indirect immunostaining of PFA fixed KO mouse brain section (hypothalamus) with Guinea pig anti-Orexin A (cat. no. 389 004, dilution 1:500 red).
Courtesy: Dana Mayer & Markus Fendt, Institute of Pharmacology and Toxicology, Magedeburg
Indirect immunostaining of PFA fixed mouse brain section with Guinea pig anti-Orexin A (cat. no. 389 004, dilution 1:500; red). Nuclei have been visualized by DAPI staining (blue).
Indirect immunostaining of PFA fixed mouse brain section with Guinea pig anti-Orexin A (cat. no. 389 004, dilution 1:500; red). Nuclei have been visualized by DAPI staining (blue).
| Cat. No. | Product Description | Application | Quantity | Price | Cart |
|---|
| 434 005 | Neuropeptide S, Guinea pig, polyclonal, affinity purifiedaffinity | IHC IHC-P | 50 µg | $465.00 |   |
| 394 004 | Neuropeptide Y, Guinea pig, polyclonal, antiserumantiserum | ICC IHC IHC-P | 100 µl | $370.00 | |
| 394 006 | Neuropeptide Y, chicken, polyclonal, affinity purifiedaffinity K.O. | ICC IHC | 200 µl | $385.00 |  |
| 418 005 | Neurotensin, Guinea pig, polyclonal, affinity purifiedaffinity | IHC IHC-P | 50 µg | $465.00 | |
| 389 004 | Orexin A, Guinea pig, polyclonal, antiserumantiserum K.O. | IHC IHC-P | 100 µl | $370.00 | |
| 389 104 | Orexin A/B, Guinea pig, polyclonal, antiserumantiserum | IHC IHC-P | 100 µl | $370.00 | |
| 408 004 | Oxytocin, Guinea pig, polyclonal, antiserumantiserum K.O. | IHC IHC-P | 100 µl | $375.00 | |
| 408 017 | Oxytocin, rat, monoclonal, purified IgG IgG | Dot blot IHC | 100 µg | $420.00 | |
| 366 004 | Somatostatin-28, Guinea pig, polyclonal, antiserumantiserum | ICC IHC IHC-P | 100 µl | $375.00 | |
| 366 006 | Somatostatin-28, chicken, polyclonal, affinity purifiedaffinity | ICC IHC IHC-P | 200 µl | $385.00 | |
| 366 017 | Somatostatin-28, rat, monoclonal, purified IgG IgG | ICC IHC IHC-P | 100 µg | $420.00 | |
| 459 005 | Substance P, Guinea pig, polyclonal, affinity purifiedaffinity | ICC IHC | 50 µg | $465.00 | |
| 403 004 | Vasopressin, Guinea pig, polyclonal, antiserumantiserum | IHC IHC-P | 100 µl | $375.00 | |
| 443 005 | VIP, Guinea pig, polyclonal, affinity purifiedaffinity | ICC IHC IHC-P | 50 µg | $470.00 | |
