Synaptic Systems - Substance P

Cat. No.: 459 005

Amount: 50 µg

Price: $460.00

IHC

Background

Substance P (also designated SP or NK-1) is a highly conserved peptide that was originally discovered in 1931 (1). It is an undecapeptide belonging to the tachykinin neuropeptide family. Mammalian tachykinins include substance P, neurokinin A and neurokinin B, and they all share the same carboxy-terminal amino acid sequence (2,3).
Substance P is generated by post-translational cleavage of the precursor Protachykinin-1 (2,3).
Substance P is widely distributed throughout the central and peripheral nervous system, including the brain, spinal cord, and nerve fibers and endocrine cells of the gastrointestinal tract (3,4).
Substance P exerts its effects by binding to the neurokinin-1 receptor NK-1R (2,3).
It functions in the central and peripheral nervous systems as a neurotransmitter or neuromodulator (5) and is involved in many biological processes, including intestinal smooth muscle contraction, blood pressure regulation, neurogenic inflammation, nociception and pain transmission (2,5). Furthermore, it has gained attention for its role in complex psychiatric processes including stress, anxiety, and depression (2).

1 ) V Euler US et al., J Physiol (1931)
2 ) Schank JR et al., Int Rev Neurobiol (2017)
3 ) Koon HW et al., Ann N Y Acad Sci (2006)
4 ) Ribeiro-da-Silva A et al., Neuropeptides (2000)
5 ) Otsuka M et al., Physiol Rev (1993)

Protocols

IHC

Cat. No. 459 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 58 to 68 from mouse Protachykinin-1 (UniProt Id: P41539)
Reactivity	Reacts with: mouse (P41539), rat (P06767). Other species not tested yet.
Specificity	The antibody is specific for substance P. It shows no cross-reactivity to neurokinin A, neurokinin B, or the unprocessed precursor protein.
Data sheet	459_005.pdf

Substance P antibody - 459 005