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Vasopressin antibody - 403 004

Vasopressin is a peptide hormone with effects on water homeostasis, blood pressure, cognition, and social behavior
Guinea pig polyclonal antiserum
Cat. No.: 403 004
Amount: 100 µl
Price: $370.00
Cat. No. 403 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500      gallery  
IHC-P: 1 : 500 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Synthetic peptide corresponding to AA 24 to 32 from mouse Vasopressin-neurophysin2-copeptin (UniProt Id: P35455)
Reactivity Reacts with: mouse (P35455), rat (P01186).
Other species not tested yet.
Specificity The antibody recognizes Arginine-vasopressin. It may crossreact with the unprocessed precursor protein.
Data sheet 403_004.pdf

References for Vasopressin - 403 004

Long-range projections of oxytocin neurons in the marmoset brain.
Lefevre A, Meza J, Miller CT
Journal of neuroendocrinology (2024) : e13397. 403 004 IHC; tested species: marmoset
NaX channel is a physiological [Na+] detector in oxytocin and vasopressin releasing magnocellular neurosecretory cells of the rat supraoptic nucleus.
Salgado-Mozo S, Thirouin ZS, Wyrosdic JC, García-Hernández U, Bourque CW
The Journal of neuroscience : the official journal of the Society for Neuroscience (2023) : . 403 004 IHC; tested species: rat
Effects of Salt Loading on the Organization of Microtubules in Rat Magnocellular Vasopressin Neurons.
Hicks AI, Barad Z, Sobrero A, Lean G, Jacob-Tomas S, Yang J, Choe KY, Prager-Khoutorsky M
Journal of neuroendocrinology (2019) : e12817. 403 004 IHC; tested species: rat
Unique Organization of Actin Cytoskeleton in Magnocellular Vasopressin Neurons in Normal Conditions and in Response to Salt-Loading.
Barad Z, Jacob-Tomas S, Sobrero A, Lean G, Hicks AI, Yang J, Choe KY, Prager-Khoutorsky M
eNeuro () 72: . 403 004 IHC; tested species: mouse
Cat. No.: 403 004
Amount: 100 µl
Price: $370.00
Long-range projections of oxytocin neurons in the marmoset brain.
Lefevre A, Meza J, Miller CT
Journal of neuroendocrinology (2024) : e13397. 403 004 IHC; tested species: marmoset
NaX channel is a physiological [Na+] detector in oxytocin and vasopressin releasing magnocellular neurosecretory cells of the rat supraoptic nucleus.
Salgado-Mozo S, Thirouin ZS, Wyrosdic JC, García-Hernández U, Bourque CW
The Journal of neuroscience : the official journal of the Society for Neuroscience (2023) : . 403 004 IHC; tested species: rat
Effects of Salt Loading on the Organization of Microtubules in Rat Magnocellular Vasopressin Neurons.
Hicks AI, Barad Z, Sobrero A, Lean G, Jacob-Tomas S, Yang J, Choe KY, Prager-Khoutorsky M
Journal of neuroendocrinology (2019) : e12817. 403 004 IHC; tested species: rat
Unique Organization of Actin Cytoskeleton in Magnocellular Vasopressin Neurons in Normal Conditions and in Response to Salt-Loading.
Barad Z, Jacob-Tomas S, Sobrero A, Lean G, Hicks AI, Yang J, Choe KY, Prager-Khoutorsky M
eNeuro () 72: . 403 004 IHC; tested species: mouse
Background
Vasopressin, also referred to as arginine-vasopressin (AVP) or antidiuretic hormone (ADH), is a nine amino acid peptide hormone secreted from the posterior pituitary in response to reductions in plasma volume and increases in plasma osmolarity. It is proteolytically processed from a precursor protein that is mainly synthesized in neurons of the hypothalamus.
Along with its carrier protein, neurophysin 2, vasopressin is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis where it is either stored or secreted into the bloodstream. Its release increases the amount of solute-free water reabsorbed back into the circulation from the filtrate in the kidney tubules of the nephrons. Further, vasopressin causes vasoconstriction of peripheral vessels, which increases peripheral vascular resistance and raises arterial blood pressure.
Some vasopressin is also released directly into the brain and is involved in cognition, social behavior, sexual motivation, pair-bonding, and maternal behavior.
Mutations in the vasopressin gene cause autosomal dominant neurohypophyseal diabetes insipidus (ADNDI), which is characterised by persistant thirst, polydipsia and polyuria.