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Antibody Labeling

Primary fluorescence labeled antibodies allow the usage of several antibodies from the same species in one staining reaction since no secondary detection reagents are required. For biotinylated antibodies, alternative secondary reagents like fluochromated avidin or streptavidin can be used.

Synaptic Systems offers the labeling of antibodies with biotin or the following fluorescent dyes:

  • Sulfo-Cyanine 2, Sulfo-Cyanine 3, Sulfo-Cyanine 5
  • ATTO® 550, ATTO® 565, ATTO® 590 (suitable for STED microscopy), ATTO® 647N (suitable for STED microscopy), ATTO® 665
  • Chromeo® dyes
  • Mega Stokes DY-XL® dyes
  • AbberiorStar dyes
  • CypHer5E
  • AcidiFluorORANGE

Antibodies for labeling must be provided in a purified form with a concentration of 1 mg/ml or higher without stabilizing proteins (BSA, gelatine, etc.). A minimum total amount of 0.5 mg of antibody is required.
Synaptic Systems can carry out the purification of the antibody for you as well.

For further information please contact antibodyservice@sysy.com.