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Home > Products > 434 005

Neuropeptide S antibody - 434 005

Neuropeptide S is involved in sleep/wake functions, arousal/anxiety responses, locomotion, food intake, learning and memory
Guinea pig polyclonal purified antibody
Cat. No.: 434 005
Amount: 50 µg
Price: $460.00
Cat. No. 434 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 gallery  
IHC-P: 1 : 1000 up to 1 : 2000 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to AA 70 to 89 from mouse Neuropeptide S (UniProt Id: P0C0P8)
Reactivity Reacts with: rat (P0C0P7).
Other species not tested yet.
Specificity Recognizes Neuropeptide S. It may crossreact with the unprocessed precursor protein.
Data sheet 434_005.pdf
Cat. No.: 434 005
Amount: 50 µg
Price: $460.00
IHC
IHC
IHC-P
Background

Neuropeptide S (NPS) is a 20 amino acid neuropeptide that is processed from a larger precursor. NPS was named after a conserved serine residue at its N-terminus and is found in all tetrapod vertebrates but is absent from fish.

It is predominantly expressed in the central nervous system. The distribution pattern is only partially conserved between mice and rats. In both rat and mouse brain, expression of NPS precursor mRNA is found in the locus coeruleus (LC) area. In the parabrachial area, NPS precursor mRNA is expressed in the lateral parabrachial nucleus (LPB) in the rat brain, whereas expression in the mouse brain is found in the Kölliker-Fuse (KF) nucleus. Besides these two regions, NPS precursor mRNA is also found in additional regions in the rat brain, including the principal sensory trigeminal nucleus (Pr5).

NPS-responsive neurons extend projections into many other brain areas. NPS binds to its receptor NPSR1 to increase intracellular calcium concentrations. Physiologically, NPS has been characterized as a modulator of sleep/wake functions, locomotion, arousal/anxiety responses, food intake, and learning and memory.