Somatostatin-28 antibody - 366 017

SST is a peptide hormone with inhibitory functions

Rat monoclonal purified IgG

Cat. No.: 366 017

Amount: 100 µg

Price: $420.00

Cat. No. 366 017	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet ICC: 1 : 500 up to 1 : 2000 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: 1 : 100 up to 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	SY-160F7
Subtype	IgG2b (κ light chain)
Immunogen	Synthetic peptide corresponding to AA 89 to 100 from mouse Somatostatin (UniProt Id: P60041)
Reactivity	Reacts with: rat (P60042), mouse (P60041), human (P61278). Other species not tested yet.
Specificity	The antibody detects somatostatin-28. It also recognizes the unprocessed precursor protein. It does not bind to somatostatin-14.
Data sheet	366_017.pdf

Cat. No.: 366 017

Amount: 100 µg

Price: $420.00

ICC

IHC

IHC-P

Background

Somatostatin, also referred to as SST, growth hormone-inhibiting hormone or GHIH, is a peptide hormone that regulates the endocrine system and affects neurotransmission and cell proliferation via interaction with G protein-coupled somatostatin receptors. It inhibits the secretion of many important hormones, including insulin, glucagon and somatotropin (also designated growth hormone, or GH).
Somatostatin has two forms, active 14 amino acid and 28 amino acid. They are produced by alternative cleavage of the single precursor protein encoded by this gene.

1 ) Gahete MD et al., Ann. N. Y. Acad. Sci. (2010)
2 ) Brakch N et al., Biochemistry (2002)
3 ) Dournaud P et al., J. Neurosci. (1998)
4 ) Aguila MC et al., Endocrinology (1996)
5 ) Patel YC et al., Biochem. Biophys. Res. Commun. (1994)
6 ) Gomez S et al., EMBO J. (1989)
7 ) Shen LP et al., Science (1984)

Protocols

ICC

IHC

IHC-P

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