Neuropeptide Y antibody - 394 006 K.O.

Neuropeptide Y is involved in the regulation of food intake, energy storage, stress perception and blood pressure

Chicken polyclonal purified antibody

Cat. No.: 394 006

Amount: 200 µl

Price: $380.00

For the import of chicken antibodies into the USA you need a special import permit. This can be applied for at your USDA office. If you cannot apply for this, please order the chicken antibodies through our distributor Cedarlane USA. If you have any questions, please contact us.

Cat. No. 394 006	200 µl specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 200 µl H₂O. Then aliquot and store at -20°C to -80°C until use.
Applications	WB: not recommended IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 (see remarks) gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 1 to 16 from mouse Neuropeptide Y (UniProt Id: P57774)
Reactivity	Reacts with: rat (P07808), mouse (P57774). Other species not tested yet.
Specificity	Specific for Neuropeptide Y, may cross-react with the unprocessed precursor protein. K.O. validated
Remarks	IHC: Antigen retrieval (10mM Tris, 1mM EDTA, pH 9.0, overnight at 60°C) is recommended.
Data sheet	394_006.pdf

Cat. No.: 394 006

Amount: 200 µl

Price: $380.00

ICC

IHC

Background

Neuropeptide Y (NPY) is a 36-amino acid polypeptide that acts as a neurotransmitter in the brain and in the autonomic nervous system. It is produced and secreted by neurons of the sympathetic nervous system and neurons in the brain, e.g. the hypothalamus. NPY is involved in several physiological functions, including: increasing food intake and storage of energy as fat, reducing anxiety and stress, reducing pain perception, and lowering blood pressure.

1 ) Jacques D et al., Can. J. Physiol. Pharmacol. (2017)
2 ) Shi Z et al., J. Clin. Invest. (2017)
3 ) Rodnoi P et al., JCI Insight (2017)
4 ) Kanevskij M et al., Cardiovasc. Res. (2002)
5 ) Compan V et al., Brain Res. (1998)

Protocols

ICC

IHC

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