Synaptic Systems - Orexin A

Cat. No.: 389 004

Amount: 100 µl

Price: $365.00

Cat. No. 389 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 46 to 65 from mouse Orexin (UniProt Id: O55241)
Reactivity	Reacts with: human, rat, mouse (O55241). Other species not tested yet.
Specificity	Recognizes Orexin A with only minor cross-reactivity to the unprocessed precursor protein. Does not cross-react to Orexin B. K.O. validated
Data sheet	389_004.pdf

References for Orexin A - 389 004

Orexin neurons play contrasting roles in itch and pain neural processing via projecting to the periaqueductal gray.
Kaneko T, Oura A, Imai Y, Kusumoto-Yoshida I, Kanekura T, Okuno H, Kuwaki T, Kashiwadani H
Communications biology (2024) 71: 290. 389 004 IHC; tested species: mouse

Lesion of NPY Receptor-expressing Neurons in Perifornical Lateral Hypothalamus Attenuates Glucoprivic Feeding.
Choi PP, Wang Q, Brenner LA, Li AJ, Ritter RC, Appleyard SM
Endocrinology (2024) 1655: . 389 004 IHC; tested species: rat

Esr1+ hypothalamic-habenula neurons shape aversive states.
Calvigioni D, Fuzik J, Le Merre P, Slashcheva M, Jung F, Ortiz C, Lentini A, Csillag V, Graziano M, Nikolakopoulou I, Weglage M, et al.
Nature neuroscience (2023) 267: 1245-1255. 389 004 IHC; tested species: mouse

Hypothalamic orexinergic neurons modulate pain and itch in an opposite way: pain relief and itch exacerbation.
Kaneko T, Kuwaki T, Kashiwadani H
The journal of physiological sciences : JPS (2022) 721: 21. 389 004 IHC; tested species: mouse

Orexin (hypocretin) participates in central autonomic regulation during fight-or-flight response.
Kuwaki T
Peptides (2021) 139: 170530. 389 004 IHC; tested species: mouse

Cat. No.: 389 004

Amount: 100 µl

Price: $365.00

Orexin (hypocretin) participates in central autonomic regulation during fight-or-flight response.
Kuwaki T
Peptides (2021) 139: 170530. 389 004 IHC; tested species: mouse

IHC

IHC-P

Background

Orexins, also referred to as Hypocretins, are produced by specialized neurons in the lateral hypothalamus, which project to many different regions of the brain. Orexin A (33 amino acids) and Orexin B (28 amino acids) are both produced from a single pre-pro-orexin polypeptide by a cascade of enzymatic reactions. They operate via binding to two closely related G protein-coupled receptors called Orexin receptor 1 and 2 (OxR 1 and OxR 2). OxR 1 is selective for Orexin A whereas OxR 2 binds both peptides with similar affinity.
The orexin system is conserved in mammals, and plays a central role in regulating feeding, sleep/wake cycles, arousal, energy expenditure, reward seeking, cognition, and stress responses. Defects in orexin signaling are involved in diet-induced obesity, diabetes, narcolepsy, panic anxiety disorder, depression, and addiction.

1 ) Huber MJ et al., Cell. Mol. Neurobiol. (2017)
2 ) Villano I et al., Front Behav Neurosci (2017)
3 ) Chow M et al., Nat Sci Sleep (2016)
4 ) Nixon JP et al., Ageing Res. Rev. (2015)
5 ) Hu B et al., Neurosci Biobehav Rev (2015)
6 ) Mahler SV et al., Nat. Neurosci. (2014)
7 ) Perez-Leighton CE et al., Biochim. Biophys. Acta (2014)
8 ) Alexandre C et al., Curr. Opin. Neurobiol. (2013)
9 ) Kodadek T et al., Mol Biosyst (2010)

Protocols

IHC

IHC-P

Orexin A antibody - 389 004 K.O.

References for Orexin A - 389 004