|Cat. No. 214-1P||
100 µg protein, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use.
Control proteins should be stored at +4°C when still lyophilized. Do not freeze!
|Immunogen||Recombinant protein corresponding to AA 1 to 271 from mouse Calretinin (UniProt Id: Q08331)|
|Recommended dilution||Optimal concentrations should be determined by the end-user.|
|Matching antibodies||214 102, 214 104, 214 106, 214 111, 214 111BT, 214 111C2, 214 111C3, 214 111C5, 214 211, 214 108|
This control protein consists of the recombinant protein (full length mouse calretinin) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Two isoforms of the the vitamin D-dependent Ca-binding proteins have been described so far: Calretinin, also referred to as calbindin D29k, calbindin 2, CALB 2, CAL 2, and CAB 29, and calbindin D28k. These proteins are expressed in cells that have to handle a high calcium influx such as brain, bone, teeth, inner ear and others. Calbindins are believed to regulate cellular activity by suppressing or buffering intracellur calcium.