Tailor-made Antibodies
and Tools for Life Science
quick order | cart
?
|
login
Home|||||Technical Support
Home > Products > 214 111

Calretinin antibody - 214 111

Calretinin is a vitamin D-dependent calcium binding protein
Mouse monoclonal purified IgG
Cat. No.: 214 111
Amount: 100 µg
Price: $415.00
Cat. No. 214 111 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 200 up to 1 : 1000 gallery  
IHC-P: 1 : 500    gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone 37C9
Subtype IgG1 (κ light chain)
Immunogen Full-length recombinant mouse Calretinin (UniProt Id: Q08331)
Reactivity Reacts with: rat (P47728), mouse (Q08331), human (P22676), zebrafish.
Other species not tested yet.
Specificity Specific for calretinin / calbindin D29k.
Matching control protein/peptide 214-1P
Data sheet 214_111.pdf

References for Calretinin - 214 111

Role of PKN1 in Retinal Cell Type Formation.
Brunner M, Lang L, Künkel L, Weber D, Safari MS, Baier-Bitterlich G, Zur Nedden S
International journal of molecular sciences (2024) 255: . 214 111 IHC; tested species: mouse
Neuronal integration in the adult mouse olfactory bulb is a non-selective addition process.
Platel JC, Angelova A, Bugeon S, Wallace J, Ganay T, Chudotvorova I, Deloulme JC, Béclin C, Tiveron MC, Coré N, Murthy VN, et al.
eLife (2019) 8: . 214 111 IHC; tested species: mouse
Delivery of different genes into pre- and post-synaptic neocortical interneurons connected by GABAergic synapses.
Nagayach A, Singh A, De Blas AL, Geller AI
PloS one (2019) 145: e0217094. 214 111 IHC; tested species: rat
Characterization of perinatally born glutamatergic neurons of the mouse olfactory bulb based on NeuroD6 expression reveals their resistance to sensory deprivation.
Angelova A, Platel JC, Béclin C, Cremer H, Coré N
The Journal of comparative neurology (2019) 5277: 1245-1260. 214 111 IHC; tested species: mouse
Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody.
Kaczmarek-Hajek K, Zhang J, Kopp R, Grosche A, Rissiek B, Saul A, Bruzzone S, Engel T, Jooss T, Krautloher A, Schuster S, et al.
eLife (2018) 7: . 214 111 IHC; tested species: mouse
NCAM Regulates Inhibition and Excitability in Layer 2/3 Pyramidal Cells of Anterior Cingulate Cortex.
Zhang X, Sullivan CS, Kratz MB, Kasten MR, Maness PF, Manis PB
Frontiers in neural circuits (2017) 11: 19. 214 111 IHC; tested species: mouse
Cat. No.: 214 111
Amount: 100 µg
Price: $415.00
Role of PKN1 in Retinal Cell Type Formation.
Brunner M, Lang L, Künkel L, Weber D, Safari MS, Baier-Bitterlich G, Zur Nedden S
International journal of molecular sciences (2024) 255: . 214 111 IHC; tested species: mouse
Neuronal integration in the adult mouse olfactory bulb is a non-selective addition process.
Platel JC, Angelova A, Bugeon S, Wallace J, Ganay T, Chudotvorova I, Deloulme JC, Béclin C, Tiveron MC, Coré N, Murthy VN, et al.
eLife (2019) 8: . 214 111 IHC; tested species: mouse
Delivery of different genes into pre- and post-synaptic neocortical interneurons connected by GABAergic synapses.
Nagayach A, Singh A, De Blas AL, Geller AI
PloS one (2019) 145: e0217094. 214 111 IHC; tested species: rat
Characterization of perinatally born glutamatergic neurons of the mouse olfactory bulb based on NeuroD6 expression reveals their resistance to sensory deprivation.
Angelova A, Platel JC, Béclin C, Cremer H, Coré N
The Journal of comparative neurology (2019) 5277: 1245-1260. 214 111 IHC; tested species: mouse
Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody.
Kaczmarek-Hajek K, Zhang J, Kopp R, Grosche A, Rissiek B, Saul A, Bruzzone S, Engel T, Jooss T, Krautloher A, Schuster S, et al.
eLife (2018) 7: . 214 111 IHC; tested species: mouse
NCAM Regulates Inhibition and Excitability in Layer 2/3 Pyramidal Cells of Anterior Cingulate Cortex.
Zhang X, Sullivan CS, Kratz MB, Kasten MR, Maness PF, Manis PB
Frontiers in neural circuits (2017) 11: 19. 214 111 IHC; tested species: mouse
WB
ICC
IHC
IHC-P
Background

Two isoforms of the vitamin D-dependent Ca-binding proteins have been described so far: Calretinin, also referred to as calbindin D29k, calbindin 2, CALB 2, CAL 2, and CAB 29, and calbindin D28k. These proteins are expressed in cells that have to handle a high calcium influx such as brain, bone, teeth, inner ear and others. Calbindins are believed to regulate cellular activity by suppressing or buffering intracellur calcium.
Other products for this target