|Cat. No. 214 111BT||100 µg purified IgG, lyophilized, biotin-labeled. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: not tested yet
IP: not tested yet
ICC: not tested yet
IHC: 1 : 200 up to 1 : 500 gallery
IHC-P/FFPE: 1 : 500 gallery
|Subtype||IgG1 (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 1 to 271 from mouse Calretinin (UniProt Id: Q08331)|
Epitop: AA 1 to 271 from mouse Calretinin (UniProt Id: Q08331)
Reacts with: rat (P47728), mouse (Q08331), human (P22676), zebrafish.
Other species not tested yet.
|Specificity||Specific for calretinin / calbindin D29k.|
|Matching control protein/peptide||214-1P|
Two isoforms of the the vitamin D-dependent Ca-binding proteins have been described so far: Calretinin, also referred to as calbindin D29k, calbindin 2, CALB 2, CAL 2, and CAB 29, and calbindin D28k. These proteins are expressed in cells that have to handle a high calcium influx such as brain, bone, teeth, inner ear and others. Calbindins are believed to regulate cellular activity by suppressing or buffering intracellur calcium.