Home|||||||Technical Support|

Calretinin antibody - 214 111C3

Calretinin is a vitamin D-dependent calcium binding protein
Monoclonal mouse purified IgG
Cat. No.: 214 111C3
Amount: 100 µg
Price: $465.00
Cat. No. 214 111C3 100 µg purified IgG, lyophilized, fluorescence-labeled with Oyster® 550

Fluorescence labeled antibodies conjugated to Oyster dyes from Luminartis GmbH (formerly Denovo Biolabels) allow the usage of established CyDye fluorescence detection systems. Oyster-488 has similar exitation and emission properties as Cy2, Oyster-550 as Cy3, and Oyster-650 is comparable to Cy5.
For our custom antibody labeling service Oyster-500 is also available.
Oyster dyes do not form dimers and therefore exhibit less internal quenching. This allows a higher labeling degree and consequently brighter conjugates.

Oyster dyes, Oyster-488, Oyster-500, Oyster-550, and Oyster-650 are trademarks of Luminartis GmbH, Muenster/Germany.

CyDyes, Cy2, Cy3, and Cy5 are trademarks of GE-Healthcare Ltd.

. Albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.The mounting agent Aquatex®(Merck Chemicals) is not compatible with Oyster dyes!
Applications WB: N/A
IP: N/A
ICC: 1 : 500 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P/FFPE: not tested yet
Label Oyster 550
Clone 37C9
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 271 from mouse Calretinin (UniProt Id: Q08331)
Epitop Epitop: AA 1 to 271 from mouse Calretinin (UniProt Id: Q08331)
Reactivity Reacts with: rat (P47728), mouse (Q08331), human (P22676), zebrafish.
Other species not tested yet.
Specificity Specific for calretinin / calbindin D29k.
Matching control protein/peptide 214-1P
Data sheet 214_111c3.pdf

References for Calretinin - 214 111C3

Zic-Proteins Are Repressors of Dopaminergic Forebrain Fate in Mice and C. elegans.
Tiveron MC, Beclin C, Murgan S, Wild S, Angelova A, Marc J, Coré N, de Chevigny A, Herrera E, Bosio A, Bertrand V, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3744: 10611-10623. 214 111C3 IHC; tested species: mouse
Loss of Hippocampal Calretinin and Parvalbumin Interneurons in the 5XFAD Mouse Model of Alzheimer's Disease.
Giesers NK, Wirths O
ASN neuro () 12: 1759091420925356. 214 111C3 IHC; tested species: mouse
Cat. No.: 214 111C3
Quantity: 100 µg
Price: $465.00
Zic-Proteins Are Repressors of Dopaminergic Forebrain Fate in Mice and C. elegans.
Tiveron MC, Beclin C, Murgan S, Wild S, Angelova A, Marc J, Coré N, de Chevigny A, Herrera E, Bosio A, Bertrand V, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3744: 10611-10623. 214 111C3 IHC; tested species: mouse
Loss of Hippocampal Calretinin and Parvalbumin Interneurons in the 5XFAD Mouse Model of Alzheimer's Disease.
Giesers NK, Wirths O
ASN neuro () 12: 1759091420925356. 214 111C3 IHC; tested species: mouse
Background

Two isoforms of the the vitamin D-dependent Ca-binding proteins have been described so far: Calretinin, also referred to as calbindin D29k, calbindin 2, CALB 2, CAL 2, and CAB 29, and calbindin D28k. These proteins are expressed in cells that have to handle a high calcium influx such as brain, bone, teeth, inner ear and others. Calbindins are believed to regulate cellular activity by suppressing or buffering intracellur calcium.