Calretinin antibody - 214 308

Calretinin is a vitamin D-dependent calcium binding protein

Guinea pig monoclonal recombinant IgG

Cat. No.: 214 308

Amount: 50 µg

Price: $415.00

Cat. No. 214 308	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 up to 1 : 1000 (AP-staining) gallery IP: not tested yet ICC: 1 : 500 up to 1 : 2000 gallery IHC: 1 : 500 gallery IHC-P: not tested yet IHC-G: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on glyoxal fixed (IHC-G) tissue. The tissue is perfused with saline and afterwards immersion fixed with a glyoxal solution. For details of the glyoxal solution, please refer to the remarks section. Immunoreactivity is usually revealed by fluorescence.
Clone	Gp37C9
Subtype	IgG2 (κ light chain)
Immunogen	Full-length recombinant mouse Calretinin (UniProt Id: Q08331)
Reactivity	Reacts with: rat (P47728), mouse (Q08331), human (P22676), zebrafish. Other species not tested yet.
Matching control protein/peptide	214-1P
Remarks	This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 37C9. The constant regions of the heavy and light chains have been replaced by guinea pig specific sequences. Therefore, the antibody can be used with standard anti-guinea pig secondary reagents. The antibody has been expressed in mammalian cells.
Data sheet	214_308.pdf

Cat. No.: 214 308

Amount: 50 µg

Price: $415.00

ICC

IHC

IHC-G

Background

Two isoforms of the vitamin D-dependent Ca-binding proteins have been described so far: Calretinin, also referred to as calbindin D29k, calbindin 2, CALB 2, CAL 2, and CAB 29, and calbindin D28k. These proteins are expressed in cells that have to handle a high calcium influx such as brain, bone, teeth, inner ear and others. Calbindins are believed to regulate cellular activity by suppressing or buffering intracellur calcium.

1 ) Klejbor I et al., J. Physiol. Pharmacol. (2006)
2 ) Haworth R et al., Exp. Toxicol. Pathol. (2006)
3 ) Schmidt H et al., J. Physiol. (Lond.) (2003)
4 ) Barski JJ et al., J. Neurosci. (2003)
5 ) Schwaller B et al., Cerebellum (2002)
6 ) Faucheux C et al., Biochem. J. (1998)
7 ) Lee YS et al., Proc. Natl. Acad. Sci. U.S.A. (1987)

Protocols

ICC

IHC

IHC-G

OTHER

Preabsorbtion

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