|Cat. No. 214 211||100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 (AP staining) gallery
ICC: 1 : 500 gallery
IHC: 1 : 200 up to 1 : 500 gallery
IHC-P/FFPE: 1 : 200 gallery
|Subtype||IgG2b (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 1 to 271 from mouse Calretinin (UniProt Id: Q08331)|
Epitop: AA 1 to 271 from mouse Calretinin (UniProt Id: Q08331)
Reacts with: rat (P07171, P47728), mouse (P12658, Q08331), grashopper.
No signal: zebrafish.
Other species not tested yet.
|Specificity||Recognizes both isoforms calbindin D28k and D29k / calretinin.|
|Matching control protein/peptide||214-1P|
Two isoforms of the vitamin D-dependent Ca-binding proteins have been described so far: calbindin D28k, also referred to as CALB 1, D-28k, and CAB 27, and calbindin D29k, also known as calretinin. These proteins are expressed in cells that have to handle a high calcium influx such as brain, bone, teeth, inner ear and others. Calbindins are believed to regulate cellular activity by suppressing or buffering intracellur calcium