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Calretinin antibody - 214 104

Calretinin is a vitamin D-dependent calcium binding protein
Guinea pig polyclonal antiserum
Cat. No.: 214 104
Amount: 100 µl
Price: $370.00
Cat. No. 214 104 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 200 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Full-length recombinant mouse Calretinin (UniProt Id: Q08331)
Reactivity Reacts with: rat (P47728), mouse (Q08331), chicken, zebrafish.
Other species not tested yet.
Matching control protein/peptide 214-1P
Data sheet 214_104.pdf

References for Calretinin - 214 104

Maturation of type I and type II rat vestibular hair cells in vivo and in vitro.
Borrajo M, Sedano D, Palou A, Giménez-Esbrí V, Barrallo-Gimeno A, Llorens J
Frontiers in cell and developmental biology (2024) 12: 1404894. 214 104 IHC; tested species: rat
The vestibular calyceal junction is dismantled following subchronic streptomycin in rats and sensory epithelium stress in humans.
Maroto AF, Borrajo M, Prades S, Callejo À, Amilibia E, Pérez-Grau M, Roca-Ribas F, Castellanos E, Barrallo-Gimeno A, Llorens J
Archives of toxicology (2023) 977: 1943-1961. 214 104 IHC; tested species: rat
Apical-basal distribution of different subtypes of spiral ganglion neurons in the cochlea and the changes during aging.
Wang M, Lin S, Xie R
PloS one (2023) 1810: e0292676. 214 104 IHC; tested species: mouse
Multisensory gaze stabilization in response to subchronic alteration of vestibular type I hair cells.
Schenberg L, Palou A, Simon F, Bonnard T, Barton CE, Fricker D, Tagliabue M, Llorens J, Beraneck M
eLife (2023) 12: . 214 104 IHC; tested species: mouse
Total Number and Ratio of GABAergic Neuron Types in the Mouse Lateral and Basal Amygdala.
Vereczki VK, Müller K, Krizsán É, Máté Z, Fekete Z, Rovira-Esteban L, Veres JM, Erdélyi F, Hájos N
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) 4121: 4575-4595. 214 104 IHC; tested species: mouse
Contribution of GABAergic interneurons to amyloid-β plaque pathology in an APP knock-in mouse model.
Rice HC, Marcassa G, Chrysidou I, Horré K, Young-Pearse TL, Müller UC, Saito T, Saido TC, Vassar R, de Wit J, De Strooper B, et al.
Molecular neurodegeneration (2020) 151: 3. 214 104 IHC; tested species: mouse
Defining the Vulnerability Window of Anesthesia-Induced Neuroapoptosis in Developing Dentate Gyrus Granule Cells - A Transgenic Approach Utilizing POMC-EGFP Mice.
Wei K, Chen P, Shen FY, Zhang Y, Liu YH, Wang ZR, Loepke AW, Wang YW, Deng M
Neuroscience (2019) : . 214 104 IHC; tested species: mouse
Stable Adult Hippocampal Neurogenesis in Cannabinoid Receptor CB2 Deficient Mice.
Mensching L, Djogo N, Keller C, Rading S, Karsak M
International journal of molecular sciences (2019) 2015: . 214 104 IHC; tested species: mouse
Secretagogin expression in the vertebrate brainstem with focus on the noradrenergic system and implications for Alzheimer's disease.
Zahola P, Hanics J, Pintér A, Máté Z, Gáspárdy A, Hevesi Z, Echevarria D, Adori C, Barde S, Törőcsik B, Erdélyi F, et al.
Brain structure & function (2019) : . 214 104 IHC; tested species: rat
VIP-immunoreactive interneurons within circuits of the mouse basolateral amygdala.
Rhomberg T, Rovira-Esteban L, Vikór A, Paradiso E, Kremser C, Nagy-Pál P, Papp OI, Tasan R, Erdélyi F, Szabó G, Ferraguti F, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) : . 214 104 IHC; tested species: mouse
Non-canonical heterogeneous cellular distribution and co-localization of CaMKIIα and CaMKIIβ in the spinal superficial dorsal horn.
Larsson M
Brain structure & function (2018) 2233: 1437-1457. 214 104 IHC; tested species: rat
Multiple amygdaloid divisions of arcopallium send convergent projections to the nucleus accumbens and neighboring subpallial amygdala regions in the domestic chicken: a selective pathway tracing and reconstruction study.
Hanics J, Teleki G, Alpár A, Székely AD, Csillag A
Brain structure & function (2017) 2221: 301-315. 214 104 IHC
Developmental abnormalities of cortical interneurons precede symptoms onset in a mouse model of Rett syndrome.
Tomassy GS, Morello N, Calcagno E, Giustetto M
Journal of neurochemistry (2014) 1311: 115-27. 214 104 IHC; tested species: mouse
Dentate gyrus network dysfunctions precede the symptomatic phase in a genetic mouse model of seizures.
Toader O, Forte N, Orlando M, Ferrea E, Raimondi A, Baldelli P, Benfenati F, Medrihan L
Frontiers in cellular neuroscience (2013) 7: 138. 214 104 IHC; tested species: mouse
Cell age-specific vulnerability of neurons to anesthetic toxicity.
Hofacer RD, Deng M, Ward CG, Joseph B, Hughes EA, Jiang C, Danzer SC, Loepke AW
Annals of neurology (2013) 736: 695-704. 214 104 IHC
Synaptic Organization of VGLUT3 Expressing Low-Threshold Mechanosensitive C Fiber Terminals in the Rodent Spinal Cord.
Larsson M, Broman J
eNeuro () 61: . 214 104 IHC; tested species: rat
Loss of Mecp2 Causes Atypical Synaptic and Molecular Plasticity of Parvalbumin-Expressing Interneurons Reflecting Rett Syndrome-Like Sensorimotor Defects.
Morello N, Schina R, Pilotto F, Phillips M, Melani R, Plicato O, Pizzorusso T, Pozzo-Miller L, Giustetto M
eNeuro () 55: . 214 104 IHC; tested species: mouse
Cat. No.: 214 104
Amount: 100 µl
Price: $370.00
Maturation of type I and type II rat vestibular hair cells in vivo and in vitro.
Borrajo M, Sedano D, Palou A, Giménez-Esbrí V, Barrallo-Gimeno A, Llorens J
Frontiers in cell and developmental biology (2024) 12: 1404894. 214 104 IHC; tested species: rat
The vestibular calyceal junction is dismantled following subchronic streptomycin in rats and sensory epithelium stress in humans.
Maroto AF, Borrajo M, Prades S, Callejo À, Amilibia E, Pérez-Grau M, Roca-Ribas F, Castellanos E, Barrallo-Gimeno A, Llorens J
Archives of toxicology (2023) 977: 1943-1961. 214 104 IHC; tested species: rat
Apical-basal distribution of different subtypes of spiral ganglion neurons in the cochlea and the changes during aging.
Wang M, Lin S, Xie R
PloS one (2023) 1810: e0292676. 214 104 IHC; tested species: mouse
Multisensory gaze stabilization in response to subchronic alteration of vestibular type I hair cells.
Schenberg L, Palou A, Simon F, Bonnard T, Barton CE, Fricker D, Tagliabue M, Llorens J, Beraneck M
eLife (2023) 12: . 214 104 IHC; tested species: mouse
Total Number and Ratio of GABAergic Neuron Types in the Mouse Lateral and Basal Amygdala.
Vereczki VK, Müller K, Krizsán É, Máté Z, Fekete Z, Rovira-Esteban L, Veres JM, Erdélyi F, Hájos N
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) 4121: 4575-4595. 214 104 IHC; tested species: mouse
Contribution of GABAergic interneurons to amyloid-β plaque pathology in an APP knock-in mouse model.
Rice HC, Marcassa G, Chrysidou I, Horré K, Young-Pearse TL, Müller UC, Saito T, Saido TC, Vassar R, de Wit J, De Strooper B, et al.
Molecular neurodegeneration (2020) 151: 3. 214 104 IHC; tested species: mouse
Defining the Vulnerability Window of Anesthesia-Induced Neuroapoptosis in Developing Dentate Gyrus Granule Cells - A Transgenic Approach Utilizing POMC-EGFP Mice.
Wei K, Chen P, Shen FY, Zhang Y, Liu YH, Wang ZR, Loepke AW, Wang YW, Deng M
Neuroscience (2019) : . 214 104 IHC; tested species: mouse
Stable Adult Hippocampal Neurogenesis in Cannabinoid Receptor CB2 Deficient Mice.
Mensching L, Djogo N, Keller C, Rading S, Karsak M
International journal of molecular sciences (2019) 2015: . 214 104 IHC; tested species: mouse
Secretagogin expression in the vertebrate brainstem with focus on the noradrenergic system and implications for Alzheimer's disease.
Zahola P, Hanics J, Pintér A, Máté Z, Gáspárdy A, Hevesi Z, Echevarria D, Adori C, Barde S, Törőcsik B, Erdélyi F, et al.
Brain structure & function (2019) : . 214 104 IHC; tested species: rat
VIP-immunoreactive interneurons within circuits of the mouse basolateral amygdala.
Rhomberg T, Rovira-Esteban L, Vikór A, Paradiso E, Kremser C, Nagy-Pál P, Papp OI, Tasan R, Erdélyi F, Szabó G, Ferraguti F, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) : . 214 104 IHC; tested species: mouse
Non-canonical heterogeneous cellular distribution and co-localization of CaMKIIα and CaMKIIβ in the spinal superficial dorsal horn.
Larsson M
Brain structure & function (2018) 2233: 1437-1457. 214 104 IHC; tested species: rat
Multiple amygdaloid divisions of arcopallium send convergent projections to the nucleus accumbens and neighboring subpallial amygdala regions in the domestic chicken: a selective pathway tracing and reconstruction study.
Hanics J, Teleki G, Alpár A, Székely AD, Csillag A
Brain structure & function (2017) 2221: 301-315. 214 104 IHC
Developmental abnormalities of cortical interneurons precede symptoms onset in a mouse model of Rett syndrome.
Tomassy GS, Morello N, Calcagno E, Giustetto M
Journal of neurochemistry (2014) 1311: 115-27. 214 104 IHC; tested species: mouse
Dentate gyrus network dysfunctions precede the symptomatic phase in a genetic mouse model of seizures.
Toader O, Forte N, Orlando M, Ferrea E, Raimondi A, Baldelli P, Benfenati F, Medrihan L
Frontiers in cellular neuroscience (2013) 7: 138. 214 104 IHC; tested species: mouse
Cell age-specific vulnerability of neurons to anesthetic toxicity.
Hofacer RD, Deng M, Ward CG, Joseph B, Hughes EA, Jiang C, Danzer SC, Loepke AW
Annals of neurology (2013) 736: 695-704. 214 104 IHC
Synaptic Organization of VGLUT3 Expressing Low-Threshold Mechanosensitive C Fiber Terminals in the Rodent Spinal Cord.
Larsson M, Broman J
eNeuro () 61: . 214 104 IHC; tested species: rat
Loss of Mecp2 Causes Atypical Synaptic and Molecular Plasticity of Parvalbumin-Expressing Interneurons Reflecting Rett Syndrome-Like Sensorimotor Defects.
Morello N, Schina R, Pilotto F, Phillips M, Melani R, Plicato O, Pizzorusso T, Pozzo-Miller L, Giustetto M
eNeuro () 55: . 214 104 IHC; tested species: mouse
Background

Two isoforms of the vitamin D-dependent Ca-binding proteins have been described so far: Calretinin, also referred to as calbindin D29k, calbindin 2, CALB 2, CAL 2, and CAB 29, and calbindin D28k. These proteins are expressed in cells that have to handle a high calcium influx such as brain, bone, teeth, inner ear and others. Calbindins are believed to regulate cellular activity by suppressing or buffering intracellur calcium.