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Calretinin antibody - 214 111C5

Calretinin is a vitamin D-dependent calcium binding protein
Monoclonal mouse purified IgG
Cat. No.: 214 111C5
Amount: 100 µg
Price: $465.00
Cat. No. 214 111C5 100 µg purified IgG, lyophilized, fluorescence-labeled with Oyster® 650

Fluorescence labeled antibodies conjugated to Oyster dyes from Luminartis GmbH (formerly Denovo Biolabels) allow the usage of established CyDye fluorescence detection systems. Oyster-488 has similar exitation and emission properties as Cy2, Oyster-550 as Cy3, and Oyster-650 is comparable to Cy5.
For our custom antibody labeling service Oyster-500 is also available.
Oyster dyes do not form dimers and therefore exhibit less internal quenching. This allows a higher labeling degree and consequently brighter conjugates.

Oyster dyes, Oyster-488, Oyster-500, Oyster-550, and Oyster-650 are trademarks of Luminartis GmbH, Muenster/Germany.

CyDyes, Cy2, Cy3, and Cy5 are trademarks of GE-Healthcare Ltd.

. Albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.The mounting agent Aquatex®(Merck Chemicals) is not compatible with Oyster dyes!
Applications WB: N/A
IP: N/A
ICC: 1 : 500 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P/FFPE: not tested yet
Label Oyster 650
Clone 37C9
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 271 from mouse Calretinin (UniProt Id: Q08331)
Epitop Epitop: AA 1 to 271 from mouse Calretinin (UniProt Id: Q08331)
Reactivity Reacts with: rat (P47728), mouse (Q08331), human (P22676), zebrafish.
Other species not tested yet.
Specificity Specific for calretinin / calbindin D29k.
Matching control protein/peptide 214-1P
Data sheet 214_111c5.pdf
Cat. No.: 214 111C5
Quantity: 100 µg
Price: $465.00
Background

Two isoforms of the the vitamin D-dependent Ca-binding proteins have been described so far: Calretinin, also referred to as calbindin D29k, calbindin 2, CALB 2, CAL 2, and CAB 29, and calbindin D28k. These proteins are expressed in cells that have to handle a high calcium influx such as brain, bone, teeth, inner ear and others. Calbindins are believed to regulate cellular activity by suppressing or buffering intracellur calcium.