Calretinin antibody - 214 111C2

Calretinin is a vitamin D-dependent calcium binding protein

Mouse monoclonal purified IgG

Cat. No.: 214 111C2

Amount: 100 µg

Price: $465.00

Cat. No. 214 111C2	100 µg purified IgG, lyophilized, fluorescence-labeled with Cyanine 2. Fluorescence labeled antibodies conjugated to (Sulfo-)Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy. Cyanine 2: λ_ex 492 nm / λ_em 508 nm Sulfo-Cyanine 3: λ_ex 554 nm / λ_em 568 nm Sulfo-Cyanine 5: λ_ex 649 nm / λ_em 667 nm (Sulfo-)Cyanine 5 dyes are well suited for STORM high resolution microscopy. Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates. Albumin was added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	WB: N/A IP: N/A ICC: 1 : 500 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	Cyanine 2
Clone	37C9
Subtype	IgG1 (κ light chain)
Immunogen	Full-length recombinant mouse Calretinin (UniProt Id: Q08331)
Reactivity	Reacts with: rat (P47728), mouse (Q08331), human (P22676), zebrafish. Other species not tested yet.
Specificity	Specific for calretinin / calbindin D29k.
Matching control protein/peptide	214-1P
Data sheet	214_111c2.pdf

Cat. No.: 214 111C2

Amount: 100 µg

Price: $465.00

ICC

IHC

Background

Two isoforms of the vitamin D-dependent Ca-binding proteins have been described so far: Calretinin, also referred to as calbindin D29k, calbindin 2, CALB 2, CAL 2, and CAB 29, and calbindin D28k. These proteins are expressed in cells that have to handle a high calcium influx such as brain, bone, teeth, inner ear and others. Calbindins are believed to regulate cellular activity by suppressing or buffering intracellur calcium.

1 ) Klejbor I et al., J. Physiol. Pharmacol. (2006)
2 ) Haworth R et al., Exp. Toxicol. Pathol. (2006)
3 ) Schmidt H et al., J. Physiol. (Lond.) (2003)
4 ) Barski JJ et al., J. Neurosci. (2003)
5 ) Schwaller B et al., Cerebellum (2002)
6 ) Faucheux C et al., Biochem. J. (1998)
7 ) Lee YS et al., Proc. Natl. Acad. Sci. U.S.A. (1987)

Protocols

ICC

IHC

OTHER

Preabsorbtion

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