|Cat. No. 214 111C2||
100 µg purified IgG, lyophilized. Albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
ICC: 1 : 500 gallery
IHC: 1 : 200 up to 1 : 500 gallery
IHC-P/FFPE: not tested yet
|Subtype||IgG1 (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 1 to 271 from mouse Calretinin (UniProt Id: Q08331)|
Epitop: AA 1 to 271 from mouse Calretinin (UniProt Id: Q08331)
Reacts with: rat (P47728), mouse (Q08331), human (P22676), zebrafish.
Other species not tested yet.
|Specificity||Specific for calretinin / calbindin D29k.|
|Matching control protein/peptide||214-1P|
Two isoforms of the the vitamin D-dependent Ca-binding proteins have been described so far: Calretinin, also referred to as calbindin D29k, calbindin 2, CALB 2, CAL 2, and CAB 29, and calbindin D28k. These proteins are expressed in cells that have to handle a high calcium influx such as brain, bone, teeth, inner ear and others. Calbindins are believed to regulate cellular activity by suppressing or buffering intracellur calcium.