|Cat. No. 214-0P||
100 µg protein, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use.
Control proteins should be stored at +4°C when still lyophilized. Do not freeze!
|Immunogen||Recombinant protein corresponding to AA 3 to 251 from human CalbindinD28k (UniProt Id: P05937)|
|Recommended dilution||Optimal concentrations should be determined by the end-user.|
|Matching antibodies||214 002, 214 003, 214 004, 214 005, 214 006, 214 011, 214 011BT, 214 011C3, 214 011C5, 214 008|
This control protein consists of the recombinant protein (aa 3 - 251 of human calbindin D28k) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Two isoforms of the vitamin D-dependent Ca-binding proteins have been described so far: calbindin D28k, also referred to as CALB 1, D-28k, and CAB 27, and calbindin D29k, also known as calretinin. These proteins are expressed in cells that have to handle a high calcium influx such as brain, bone, teeth, inner ear and others. Calbindins are believed to regulate cellular activity by suppressing or buffering intracellur calcium. In the brain calbindin D28k is a useful marker for specific neuronal cell types. It is particularly concentrated in the dendrites and perikarya of cerebellar Purkinje cells, but is also found in many GABAergic interneurons in the cortex.