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Calbindin D28k antibody - 214 006

Calbindin D28k is a vitamin D-dependent calcium binding protein
Chicken polyclonal IgY fraction
Cat. No.: 214 006
Amount: 200 µl
Price: $365.00
Cat. No. 214 006 200 µl antibody, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: yes
ICC: 1 : 5000 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: 1 : 200 gallery  
IHC-Fr: 1 : 500 up to 1 : 1000 (see remarks) gallery  
Immunogen Recombinant protein corresponding to AA 3 to 251 from human CalbindinD28k (UniProt Id: P05937)
Reactivity Reacts with: human (P05937), rat (P07171), mouse (P12658), monkey, ape, cow.
Other species not tested yet.
Matching control protein/peptide 214-0P
Remarks

IHC-Fr: MeOH fixation is recommended.

Data sheet 214_006.pdf

References for Calbindin D28k - 214 006

Development and Optimization of a Multilayer Rat Purkinje Neuron Culture.
Uggerud IM, Kråkenes T, Hirai H, Vedeler CA, Schubert M
Cerebellum (London, England) (2023) : . 214 006 ICC; tested species: rat
Apical-basal distribution of different subtypes of spiral ganglion neurons in the cochlea and the changes during aging.
Wang M, Lin S, Xie R
PloS one (2023) 1810: e0292676. 214 006 IHC; tested species: mouse
Fear learning and aversive stimuli differentially change excitatory synaptic transmission in perisomatic inhibitory cells of the basal amygdala.
Veres JM, Fekete Z, Müller K, Andrasi T, Rovira-Esteban L, Barabas B, Papp OI, Hajos N
Frontiers in cellular neuroscience (2023) 17: 1120338. 214 006 IHC; tested species: mouse
Calretinin-Expressing Synapses Show Improved Synaptic Efficacy with Reduced Asynchronous Release during High-Rate Activity.
Zhang C, Wang M, Lin S, Xie R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) 4213: 2729-2742. 214 006 IHC; tested species: mouse
Increased Susceptibility to Cerebral Microhemorrhages Is Associated With Imaging Signs of Microvascular Degeneration in the Retina in an Insulin-Like Growth Factor 1 Deficient Mouse Model of Accelerated Aging.
Miller LR, Tarantini S, Nyúl-Tóth Á, Johnston MP, Martin T, Bullen EC, Bickel MA, Sonntag WE, Yabluchanskiy A, Csiszar A, Ungvari ZI, et al.
Frontiers in aging neuroscience (2022) 14: 788296. 214 006 IHC; tested species: mouse
Biallelic PAX5 mutations cause hypogammaglobulinemia, sensorimotor deficits, and autism spectrum disorder.
Kaiser FMP, Gruenbacher S, Oyaga MR, Nio E, Jaritz M, Sun Q, van der Zwaag W, Kreidl E, Zopf LM, Dalm VASH, Pel J, et al.
The Journal of experimental medicine (2022) 2199: . 214 006 IHC; tested species: mouse
Molecular characteristics and laminar distribution of prefrontal neurons projecting to the mesolimbic system.
Babiczky Á, Matyas F
eLife (2022) 11: . 214 006 IHC; tested species: mouse
W246G Mutant ELOVL4 Impairs Synaptic Plasticity in Parallel and Climbing Fibers and Causes Motor Defects in a Rat Model of SCA34.
Nagaraja RY, Sherry DM, Fessler JL, Stiles MA, Li F, Multani K, Orock A, Ahmad M, Brush RS, Anderson RE, Agbaga MP, et al.
Molecular neurobiology (2021) 5810: 4921-4943. 214 006 IHC; tested species: rat
Total Number and Ratio of GABAergic Neuron Types in the Mouse Lateral and Basal Amygdala.
Vereczki VK, Müller K, Krizsán É, Máté Z, Fekete Z, Rovira-Esteban L, Veres JM, Erdélyi F, Hájos N
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) 4121: 4575-4595. 214 006 IHC; tested species: mouse
Amot and Yap1 regulate neuronal dendritic tree complexity and locomotor coordination in mice.
Rojek KO, Krzemień J, Doleżyczek H, Boguszewski PM, Kaczmarek L, Konopka W, Rylski M, Jaworski J, Holmgren L, Prószyński TJ
PLoS biology (2019) 175: e3000253. 214 006 IHC; tested species: mouse
Cat. No.: 214 006
Amount: 200 µl
Price: $365.00
Development and Optimization of a Multilayer Rat Purkinje Neuron Culture.
Uggerud IM, Kråkenes T, Hirai H, Vedeler CA, Schubert M
Cerebellum (London, England) (2023) : . 214 006 ICC; tested species: rat
Apical-basal distribution of different subtypes of spiral ganglion neurons in the cochlea and the changes during aging.
Wang M, Lin S, Xie R
PloS one (2023) 1810: e0292676. 214 006 IHC; tested species: mouse
Fear learning and aversive stimuli differentially change excitatory synaptic transmission in perisomatic inhibitory cells of the basal amygdala.
Veres JM, Fekete Z, Müller K, Andrasi T, Rovira-Esteban L, Barabas B, Papp OI, Hajos N
Frontiers in cellular neuroscience (2023) 17: 1120338. 214 006 IHC; tested species: mouse
Calretinin-Expressing Synapses Show Improved Synaptic Efficacy with Reduced Asynchronous Release during High-Rate Activity.
Zhang C, Wang M, Lin S, Xie R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) 4213: 2729-2742. 214 006 IHC; tested species: mouse
Increased Susceptibility to Cerebral Microhemorrhages Is Associated With Imaging Signs of Microvascular Degeneration in the Retina in an Insulin-Like Growth Factor 1 Deficient Mouse Model of Accelerated Aging.
Miller LR, Tarantini S, Nyúl-Tóth Á, Johnston MP, Martin T, Bullen EC, Bickel MA, Sonntag WE, Yabluchanskiy A, Csiszar A, Ungvari ZI, et al.
Frontiers in aging neuroscience (2022) 14: 788296. 214 006 IHC; tested species: mouse
Biallelic PAX5 mutations cause hypogammaglobulinemia, sensorimotor deficits, and autism spectrum disorder.
Kaiser FMP, Gruenbacher S, Oyaga MR, Nio E, Jaritz M, Sun Q, van der Zwaag W, Kreidl E, Zopf LM, Dalm VASH, Pel J, et al.
The Journal of experimental medicine (2022) 2199: . 214 006 IHC; tested species: mouse
Molecular characteristics and laminar distribution of prefrontal neurons projecting to the mesolimbic system.
Babiczky Á, Matyas F
eLife (2022) 11: . 214 006 IHC; tested species: mouse
W246G Mutant ELOVL4 Impairs Synaptic Plasticity in Parallel and Climbing Fibers and Causes Motor Defects in a Rat Model of SCA34.
Nagaraja RY, Sherry DM, Fessler JL, Stiles MA, Li F, Multani K, Orock A, Ahmad M, Brush RS, Anderson RE, Agbaga MP, et al.
Molecular neurobiology (2021) 5810: 4921-4943. 214 006 IHC; tested species: rat
Total Number and Ratio of GABAergic Neuron Types in the Mouse Lateral and Basal Amygdala.
Vereczki VK, Müller K, Krizsán É, Máté Z, Fekete Z, Rovira-Esteban L, Veres JM, Erdélyi F, Hájos N
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) 4121: 4575-4595. 214 006 IHC; tested species: mouse
Amot and Yap1 regulate neuronal dendritic tree complexity and locomotor coordination in mice.
Rojek KO, Krzemień J, Doleżyczek H, Boguszewski PM, Kaczmarek L, Konopka W, Rylski M, Jaworski J, Holmgren L, Prószyński TJ
PLoS biology (2019) 175: e3000253. 214 006 IHC; tested species: mouse
Background

Two isoforms of the vitamin D-dependent Ca-binding proteins have been described so far: calbindin D28k, also referred to as CALB 1, D-28k, and CAB 27, and calbindin D29k, also known as calretinin. These proteins are expressed in cells that have to handle a high calcium influx such as brain, bone, teeth, inner ear and others. Calbindins are believed to regulate cellular activity by suppressing or buffering intracellur calcium. In the brain calbindin D28k is a useful marker for specific neuronal cell types. It is particularly concentrated in the dendrites and perikarya of cerebellar Purkinje cells, but is also found in many GABAergic interneurons in the cortex.