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Calbindin D28k antibody - 214 005

Calbindin D28k is a vitamin D-dependent calcium binding protein
Guinea pig polyclonal purified antibody
Cat. No.: 214 005
Amount: 50 µg
Price: $465.00
Cat. No. 214 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: 1 : 500 gallery  
Immunogen Recombinant protein corresponding to AA 3 to 251 from human CalbindinD28k (UniProt Id: P05937)
Reactivity Reacts with: human (P05937), rat (P07171), mouse (P12658), monkey, ape, cow.
Other species not tested yet.
Matching control protein/peptide 214-0P
Data sheet 214_005.pdf

References for Calbindin D28k - 214 005

Development and Optimization of a Multilayer Rat Purkinje Neuron Culture.
Uggerud IM, Kråkenes T, Hirai H, Vedeler CA, Schubert M
Cerebellum (London, England) (2023) : . 214 005 ICC; tested species: rat
Immunohistochemical characterization of bipolar cells in four distantly related avian species.
Balaji V, Haverkamp S, Seth PK, Günther A, Mendoza E, Schmidt J, Herrmann M, Pfeiffer LL, Němec P, Scharff C, Mouritsen H, et al.
The Journal of comparative neurology (2023) 5314: 561-581. 214 005 IHC
Genetic disruption of bassoon in two mutant mouse lines causes divergent retinal phenotypes.
Ryl M, Urbasik A, Gierke K, Babai N, Joachimsthaler A, Feigenspan A, Frischknecht R, Stallwitz N, Fejtová A, Kremers J, von Wittgenstein J, et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2021) 355: e21520. 214 005 IHC; tested species: mouse
Adult neural stem cells have latent inflammatory potential that is kept suppressed by Tcf4 to facilitate adult neurogenesis.
Shariq M, Sahasrabuddhe V, Krishna S, Radha S, Nruthyathi , Bellampalli R, Dwivedi A, Cheramangalam R, Reizis B, Hébert J, Ghosh HS, et al.
Science advances (2021) 721: . 214 005 IHC; tested species: mouse
Maturation of Purkinje cell firing properties relies on neurogenesis of excitatory neurons.
van der Heijden ME, Lackey EP, Perez R, Ișleyen FS, Brown AM, Donofrio SG, Lin T, Zoghbi HY, Sillitoe RV
eLife (2021) 10: . 214 005 IHC; tested species: mouse
Adult brain neurons require continual expression of the schizophrenia-risk gene Tcf4 for structural and functional integrity.
Sarkar D, Shariq M, Dwivedi D, Krishnan N, Naumann R, Bhalla US, Ghosh HS
Translational psychiatry (2021) 111: 494. 214 005 IHC; tested species: mouse
Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue.
Vatan T, Minehart JA, Zhang C, Agarwal V, Yang J, Speer CM
STAR protocols (2021) 24: 100971. 214 005 IHC; tested species: mouse
Brain pathology and cerebellar purkinje cell loss in a mouse model of chronic neuronopathic Gaucher disease.
Pewzner-Jung Y, Joseph T, Blumenreich S, Vardi A, Ferreira NS, Cho SM, Eilam R, Tsoory M, Biton IE, Brumfeld V, Haffner-Krausz R, et al.
Progress in neurobiology (2020) : 101939. 214 005 IHC; tested species: mouse
Paraneoplastic cerebellar degeneration: Yo antibody alters mitochondrial calcium buffering capacity.
Panja D, Vedeler CA, Schubert M
Neuropathology and applied neurobiology (2019) 452: 141-156. 214 005 IHC; tested species: rat
Laminin β2 Chain Regulates Retinal Progenitor Cell Mitotic Spindle Orientation via Dystroglycan.
Serjanov D, Bachay G, Hunter DD, Brunken WJ
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) : . 214 005 IHC; tested species: mouse
Motor and Cerebellar Architectural Abnormalities during the Early Progression of Ataxia in a Mouse Model of SCA1 and How Early Prevention Leads to a Better Outcome Later in Life.
Ibrahim MF, Power EM, Potapov K, Empson RM
Frontiers in cellular neuroscience (2017) 11: 292. 214 005 IHC; tested species: mouse
Cat. No.: 214 005
Amount: 50 µg
Price: $465.00
Development and Optimization of a Multilayer Rat Purkinje Neuron Culture.
Uggerud IM, Kråkenes T, Hirai H, Vedeler CA, Schubert M
Cerebellum (London, England) (2023) : . 214 005 ICC; tested species: rat
Immunohistochemical characterization of bipolar cells in four distantly related avian species.
Balaji V, Haverkamp S, Seth PK, Günther A, Mendoza E, Schmidt J, Herrmann M, Pfeiffer LL, Němec P, Scharff C, Mouritsen H, et al.
The Journal of comparative neurology (2023) 5314: 561-581. 214 005 IHC
Genetic disruption of bassoon in two mutant mouse lines causes divergent retinal phenotypes.
Ryl M, Urbasik A, Gierke K, Babai N, Joachimsthaler A, Feigenspan A, Frischknecht R, Stallwitz N, Fejtová A, Kremers J, von Wittgenstein J, et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2021) 355: e21520. 214 005 IHC; tested species: mouse
Adult neural stem cells have latent inflammatory potential that is kept suppressed by Tcf4 to facilitate adult neurogenesis.
Shariq M, Sahasrabuddhe V, Krishna S, Radha S, Nruthyathi , Bellampalli R, Dwivedi A, Cheramangalam R, Reizis B, Hébert J, Ghosh HS, et al.
Science advances (2021) 721: . 214 005 IHC; tested species: mouse
Maturation of Purkinje cell firing properties relies on neurogenesis of excitatory neurons.
van der Heijden ME, Lackey EP, Perez R, Ișleyen FS, Brown AM, Donofrio SG, Lin T, Zoghbi HY, Sillitoe RV
eLife (2021) 10: . 214 005 IHC; tested species: mouse
Adult brain neurons require continual expression of the schizophrenia-risk gene Tcf4 for structural and functional integrity.
Sarkar D, Shariq M, Dwivedi D, Krishnan N, Naumann R, Bhalla US, Ghosh HS
Translational psychiatry (2021) 111: 494. 214 005 IHC; tested species: mouse
Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue.
Vatan T, Minehart JA, Zhang C, Agarwal V, Yang J, Speer CM
STAR protocols (2021) 24: 100971. 214 005 IHC; tested species: mouse
Brain pathology and cerebellar purkinje cell loss in a mouse model of chronic neuronopathic Gaucher disease.
Pewzner-Jung Y, Joseph T, Blumenreich S, Vardi A, Ferreira NS, Cho SM, Eilam R, Tsoory M, Biton IE, Brumfeld V, Haffner-Krausz R, et al.
Progress in neurobiology (2020) : 101939. 214 005 IHC; tested species: mouse
Paraneoplastic cerebellar degeneration: Yo antibody alters mitochondrial calcium buffering capacity.
Panja D, Vedeler CA, Schubert M
Neuropathology and applied neurobiology (2019) 452: 141-156. 214 005 IHC; tested species: rat
Laminin β2 Chain Regulates Retinal Progenitor Cell Mitotic Spindle Orientation via Dystroglycan.
Serjanov D, Bachay G, Hunter DD, Brunken WJ
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) : . 214 005 IHC; tested species: mouse
Motor and Cerebellar Architectural Abnormalities during the Early Progression of Ataxia in a Mouse Model of SCA1 and How Early Prevention Leads to a Better Outcome Later in Life.
Ibrahim MF, Power EM, Potapov K, Empson RM
Frontiers in cellular neuroscience (2017) 11: 292. 214 005 IHC; tested species: mouse
Background

Two isoforms of the vitamin D-dependent Ca-binding proteins have been described so far: calbindin D28k, also referred to as CALB 1, D-28k, and CAB 27, and calbindin D29k, also known as calretinin. These proteins are expressed in cells that have to handle a high calcium influx such as brain, bone, teeth, inner ear and others. Calbindins are believed to regulate cellular activity by suppressing or buffering intracellur calcium. In the brain calbindin D28k is a useful marker for specific neuronal cell types. It is particularly concentrated in the dendrites and perikarya of cerebellar Purkinje cells, but is also found in many GABAergic interneurons in the cortex.