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Calbindin D28k antibody - 214 003

Calbindin D28k is a vitamin D-dependent calcium binding protein
Rabbit polyclonal purified antibody
Cat. No.: 214 003
Amount: 50 µg
Price: $450.00
Cat. No. 214 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: yes
ICC: 1 : 5000 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  
Clarity: 1 : 300 (see remarks)
Immunogen Recombinant protein corresponding to AA 3 to 251 from human CalbindinD28k (UniProt Id: P05937)
Reactivity Reacts with: human (P05937), rat (P07171), mouse (P12658), monkey, ape, cow.
Other species not tested yet.
Specificity Specific for calbindin D28k.
Matching control protein/peptide 214-0P
Remarks

Clarity: This antibody has been successfully used for CLARITY application in human brain (Woelfle et al. 2022. bioRxiv)

Data sheet 214_003.pdf
Cat. No.: 214 003
Amount: 50 µg
Price: $450.00
Background

Two isoforms of the vitamin D-dependent Ca-binding proteins have been described so far: calbindin D28k, also referred to as CALB 1, D-28k, and CAB 27, and calbindin D29k, also known as calretinin. These proteins are expressed in cells that have to handle a high calcium influx such as brain, bone, teeth, inner ear and others. Calbindins are believed to regulate cellular activity by suppressing or buffering intracellur calcium. In the brain calbindin D28k is a useful marker for specific neuronal cell types. It is particularly concentrated in the dendrites and perikarya of cerebellar Purkinje cells, but is also found in many GABAergic interneurons in the cortex.