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Calbindin D28k - 214 011

A vitamin D-dependent calcium binding protein
Monoclonal mouse purified IgG
Cat. No.: 214 011
Amount: 100 µg
Price: $415.00
Cat. No. 214 011 100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P/FFPE: 1 : 500 gallery  
Clone 351C10
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 3 to 251 from human CalbindinD28k (UniProt Id: P05937)
Epitop Epitop: AA 3 to 251 from human CalbindinD28k (UniProt Id: P05937)
Reactivity Reacts with: human (P05937), rat (P07171), mouse (P12658), zebrafish, grashopper.
Other species not tested yet.
Specificity Specific for calbindin D28k.
Matching control protein/peptide 214-0P
Data sheet 214_011.pdf

References for Calbindin D28k - 214 011

Combinatorial analysis of calcium-binding proteins in larval and adult zebrafish primary olfactory system identifies differential olfactory bulb glomerular projection fields.
Kress S, Biechl D, Wullimann MF
Brain structure & function (2015) 2204: 1951-70. 214 011 WB, IHC; tested species: zebrafish
The Ser19Stop single nucleotide polymorphism (SNP) of human PHYHIPL affects the cerebellum in mice.
Sugimoto H, Horii T, Hirota JN, Sano Y, Shinoda Y, Konno A, Hirai H, Ishizaki Y, Hirase H, Hatada I, Furuichi T, et al.
Molecular brain (2021) 141: 52. 214 011 IHC; tested species: mouse
Genetic disruption of bassoon in two mutant mouse lines causes divergent retinal phenotypes.
Ryl M, Urbasik A, Gierke K, Babai N, Joachimsthaler A, Feigenspan A, Frischknecht R, Stallwitz N, Fejtová A, Kremers J, von Wittgenstein J, et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2021) 355: e21520. 214 011 IHC; tested species: mouse
Spatial Expression Pattern of the Major Ca2+-Buffer Proteins in Mouse Retinal Ganglion Cells.
Kovács-Öller T, Szarka G, Tengölics ÁJ, Ganczer A, Balogh B, Szabó-Meleg E, Nyitrai M, Völgyi B
Cells (2020) 94: . 214 011 IHC; tested species: mouse
Generation of human striatal organoids and cortico-striatal assembloids from human pluripotent stem cells.
Miura Y, Li MY, Birey F, Ikeda K, Revah O, Thete MV, Park JY, Puno A, Lee SH, Porteus MH, Pașca SP, et al.
Nature biotechnology (2020) 3812: 1421-1430. 214 011 IHC; tested species: human
Heterogeneity of GRIM-19 Expression in the Adult Mouse Brain.
Hwang SN, Kim JC, Kim SY
Cellular and molecular neurobiology (2019) : . 214 011 IHC; tested species: mouse
Deletion of class II ARFs in mice causes tremor by the Nav1.6 loss in cerebellar Purkinje cell axon initial segments.
Hosoi N, Shibasaki K, Hosono M, Konno A, Shinoda Y, Kiyonari H, Inoue K, Muramatsu SI, Ishizaki Y, Hirai H, Furuichi T, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) : . 214 011 IHC; tested species: mouse
Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody.
Kaczmarek-Hajek K, Zhang J, Kopp R, Grosche A, Rissiek B, Saul A, Bruzzone S, Engel T, Jooss T, Krautloher A, Schuster S, et al.
eLife (2018) 7: . 214 011 IHC; tested species: mouse
Facilitation of Contextual Fear Extinction by Orexin-1 Receptor Antagonism Is Associated with the Activation of Specific Amygdala Cell Subpopulations.
Flores Á, Herry C, Maldonado R, Berrendero F
The international journal of neuropsychopharmacology (2017) 208: 654-659. 214 011 IHC; tested species: mouse
Functional expression of calcium-permeable canonical transient receptor potential 4-containing channels promotes migration of medulloblastoma cells.
Wei WC, Huang WC, Lin YP, Becker EBE, Ansorge O, Flockerzi V, Conti D, Cenacchi G, Glitsch MD
The Journal of physiology (2017) 59516: 5525-5544. 214 011 IHC; tested species: mouse
Characterization of a novel subtype of hippocampal interneurons that express corticotropin-releasing hormone.
Hooper A, Maguire J
Hippocampus (2016) 261: 41-53. 214 011 IHC
Combinatorial analysis of calcium-binding proteins in larval and adult zebrafish primary olfactory system identifies differential olfactory bulb glomerular projection fields.
Kress S, Biechl D, Wullimann MF
Brain structure & function (2015) 2204: 1951-70. 214 011 WB, IHC; tested species: zebrafish
Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse.
Fuchs M, Brandstätter JH, Regus-Leidig H
Frontiers in cellular neuroscience (2014) 8: 60. 214 011 IHC; tested species: rat
Rescue of bilirubin-induced neonatal lethality in a mouse model of Crigler-Najjar syndrome type I by AAV9-mediated gene transfer.
Bortolussi G, Zentilin L, Baj G, Giraudi P, Bellarosa C, Giacca M, Tiribelli C, Muro AF
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2012) 263: 1052-63. 214 011 IHC; tested species: mouse
Probing the functional equivalence of otoferlin and synaptotagmin 1 in exocytosis.
Reisinger E, Bresee C, Neef J, Nair R, Reuter K, Bulankina A, Nouvian R, Koch M, Bückers J, Kastrup L, Roux I, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3113: 4886-95. 214 011 IHC; tested species: mouse
Inhibition of West Nile virus by calbindin-D28k.
Siddharthan V, Wang H, Davies CJ, Hall JO, Morrey JD
PloS one (2014) 99: e106535. 214 011 FACS
Cat. No.: 214 011
Quantity: 100 µg
Price: $415.00
Combinatorial analysis of calcium-binding proteins in larval and adult zebrafish primary olfactory system identifies differential olfactory bulb glomerular projection fields.
Kress S, Biechl D, Wullimann MF
Brain structure & function (2015) 2204: 1951-70. 214 011 WB, IHC; tested species: zebrafish
The Ser19Stop single nucleotide polymorphism (SNP) of human PHYHIPL affects the cerebellum in mice.
Sugimoto H, Horii T, Hirota JN, Sano Y, Shinoda Y, Konno A, Hirai H, Ishizaki Y, Hirase H, Hatada I, Furuichi T, et al.
Molecular brain (2021) 141: 52. 214 011 IHC; tested species: mouse
Genetic disruption of bassoon in two mutant mouse lines causes divergent retinal phenotypes.
Ryl M, Urbasik A, Gierke K, Babai N, Joachimsthaler A, Feigenspan A, Frischknecht R, Stallwitz N, Fejtová A, Kremers J, von Wittgenstein J, et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2021) 355: e21520. 214 011 IHC; tested species: mouse
Spatial Expression Pattern of the Major Ca2+-Buffer Proteins in Mouse Retinal Ganglion Cells.
Kovács-Öller T, Szarka G, Tengölics ÁJ, Ganczer A, Balogh B, Szabó-Meleg E, Nyitrai M, Völgyi B
Cells (2020) 94: . 214 011 IHC; tested species: mouse
Generation of human striatal organoids and cortico-striatal assembloids from human pluripotent stem cells.
Miura Y, Li MY, Birey F, Ikeda K, Revah O, Thete MV, Park JY, Puno A, Lee SH, Porteus MH, Pașca SP, et al.
Nature biotechnology (2020) 3812: 1421-1430. 214 011 IHC; tested species: human
Heterogeneity of GRIM-19 Expression in the Adult Mouse Brain.
Hwang SN, Kim JC, Kim SY
Cellular and molecular neurobiology (2019) : . 214 011 IHC; tested species: mouse
Deletion of class II ARFs in mice causes tremor by the Nav1.6 loss in cerebellar Purkinje cell axon initial segments.
Hosoi N, Shibasaki K, Hosono M, Konno A, Shinoda Y, Kiyonari H, Inoue K, Muramatsu SI, Ishizaki Y, Hirai H, Furuichi T, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) : . 214 011 IHC; tested species: mouse
Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody.
Kaczmarek-Hajek K, Zhang J, Kopp R, Grosche A, Rissiek B, Saul A, Bruzzone S, Engel T, Jooss T, Krautloher A, Schuster S, et al.
eLife (2018) 7: . 214 011 IHC; tested species: mouse
Facilitation of Contextual Fear Extinction by Orexin-1 Receptor Antagonism Is Associated with the Activation of Specific Amygdala Cell Subpopulations.
Flores Á, Herry C, Maldonado R, Berrendero F
The international journal of neuropsychopharmacology (2017) 208: 654-659. 214 011 IHC; tested species: mouse
Functional expression of calcium-permeable canonical transient receptor potential 4-containing channels promotes migration of medulloblastoma cells.
Wei WC, Huang WC, Lin YP, Becker EBE, Ansorge O, Flockerzi V, Conti D, Cenacchi G, Glitsch MD
The Journal of physiology (2017) 59516: 5525-5544. 214 011 IHC; tested species: mouse
Characterization of a novel subtype of hippocampal interneurons that express corticotropin-releasing hormone.
Hooper A, Maguire J
Hippocampus (2016) 261: 41-53. 214 011 IHC
Combinatorial analysis of calcium-binding proteins in larval and adult zebrafish primary olfactory system identifies differential olfactory bulb glomerular projection fields.
Kress S, Biechl D, Wullimann MF
Brain structure & function (2015) 2204: 1951-70. 214 011 WB, IHC; tested species: zebrafish
Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse.
Fuchs M, Brandstätter JH, Regus-Leidig H
Frontiers in cellular neuroscience (2014) 8: 60. 214 011 IHC; tested species: rat
Rescue of bilirubin-induced neonatal lethality in a mouse model of Crigler-Najjar syndrome type I by AAV9-mediated gene transfer.
Bortolussi G, Zentilin L, Baj G, Giraudi P, Bellarosa C, Giacca M, Tiribelli C, Muro AF
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2012) 263: 1052-63. 214 011 IHC; tested species: mouse
Probing the functional equivalence of otoferlin and synaptotagmin 1 in exocytosis.
Reisinger E, Bresee C, Neef J, Nair R, Reuter K, Bulankina A, Nouvian R, Koch M, Bückers J, Kastrup L, Roux I, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3113: 4886-95. 214 011 IHC; tested species: mouse
Inhibition of West Nile virus by calbindin-D28k.
Siddharthan V, Wang H, Davies CJ, Hall JO, Morrey JD
PloS one (2014) 99: e106535. 214 011 FACS
Background

Two isoforms of the vitamin D-dependent Ca-binding proteins have been described so far: calbindin D28k, also referred to as CALB 1, D-28k, and CAB 27, and calbindin D29k, also known as calretinin. These proteins are expressed in cells that have to handle a high calcium influx such as brain, bone, teeth, inner ear and others. Calbindins are believed to regulate cellular activity by suppressing or buffering intracellur calcium. In the brain calbindin D28k is a useful marker for specific neuronal cell types. It is particularly concentrated in the dendrites and perikarya of cerebellar Purkinje cells, but is also found in many GABAergic interneurons in the cortex.