Synaptic Systems - Calbindin D28k

Cat. No.: 214 011

Amount: 100 µg

Price: $425.00

Cat. No. 214 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: 1 : 500 up to 1 : 1000 gallery Clarity: 1 : 300 (see remarks)
Clone	351C10
Subtype	IgG1 (κ light chain)
Immunogen	Full-length recombinant human Calbindin D28k protein (UniProt Id: P05937)
Reactivity	Reacts with: human (P05937), rat (P07171), mouse (P12658), zebrafish, grashopper. Other species not tested yet.
Matching control protein/peptide	214-0P
Remarks	Clarity: This antibody has been successfully used for CLARITY application in human brain (Woelfle et al., 2023; PMID: 37221592).
Data sheet	214_011.pdf

References for Calbindin D28k - 214 011

WB (2)
IHC (19)
Clarity (2)
FACS (1)

Nrg1 haploinsufficiency alters inhibitory cortical circuits.
Navarro-Gonzalez C, Carceller H, Benito Vicente M, Serra I, Navarrete M, Domínguez-Canterla Y, Rodríguez-Prieto Á, González-Manteiga A, Fazzari P
Neurobiology of disease (2021) 157: 105442. 214 011 WB; tested species: mouse

Combinatorial analysis of calcium-binding proteins in larval and adult zebrafish primary olfactory system identifies differential olfactory bulb glomerular projection fields.
Kress S, Biechl D, Wullimann MF
Brain structure & function (2015) 2204: 1951-70. 214 011 IHC, WB; tested species: zebrafish

INSIHGT: an accessible multi-scale, multi-modal 3D spatial biology platform.
Yau CN, Hung JTS, Campbell RAA, Wong TCY, Huang B, Wong BTY, Chow NKN, Zhang L, Tsoi EPL, Tan Y, Li JJX, et al.
Nature communications (2024) 151: 10888. 214 011 IHC; tested species: mouse

A pupillary contrast response in mice and humans: Neural mechanisms and visual functions.
Fitzpatrick MJ, Krizan J, Hsiang JC, Shen N, Kerschensteiner D
Neuron (2024) : . 214 011 IHC; tested species: mouse

Sensory innervation of masseter, temporal and lateral pterygoid muscles in common marmosets.
Hovhannisyan AH, Lindquist K, Belugin S, Mecklenburg J, Ibrahim T, Tram M, Corey T, Salmon A, Ruparel S, Ruparel S, Akopian A, et al.
bioRxiv : the preprint server for biology (2023) : . 214 011 IHC; tested species: marmoset

Long-Term Effects of Biliverdin Reductase a Deficiency in Ugt1-/- Mice: Impact on Redox Status and Metabolism.
Bortolussi G, Shi X, Ten Bloemendaal L, Banerjee B, De Waart DR, Baj G, Chen W, Oude Elferink RP, Beuers U, Paulusma CC, Stocker R, et al.
Antioxidants (Basel, Switzerland) (2021) 1012: . 214 011 IHC; tested species: mouse

Regional Variation of Gap Junctional Connections in the Mammalian Inner Retina.
Fusz K, Kovács-Öller T, Kóbor P, Szabó-Meleg E, Völgyi B, Buzás P, Telkes I
Cells (2021) 109: . 214 011 IHC; tested species: mouse,rat

Genetic disruption of bassoon in two mutant mouse lines causes divergent retinal phenotypes.
Ryl M, Urbasik A, Gierke K, Babai N, Joachimsthaler A, Feigenspan A, Frischknecht R, Stallwitz N, Fejtová A, Kremers J, von Wittgenstein J, et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2021) 355: e21520. 214 011 IHC; tested species: mouse

The Ser19Stop single nucleotide polymorphism (SNP) of human PHYHIPL affects the cerebellum in mice.
Sugimoto H, Horii T, Hirota JN, Sano Y, Shinoda Y, Konno A, Hirai H, Ishizaki Y, Hirase H, Hatada I, Furuichi T, et al.
Molecular brain (2021) 141: 52. 214 011 IHC; tested species: mouse

Spatial Expression Pattern of the Major Ca2+-Buffer Proteins in Mouse Retinal Ganglion Cells.
Kovács-Öller T, Szarka G, Tengölics ÁJ, Ganczer A, Balogh B, Szabó-Meleg E, Nyitrai M, Völgyi B
Cells (2020) 94: . 214 011 IHC; tested species: mouse

Generation of human striatal organoids and cortico-striatal assembloids from human pluripotent stem cells.
Miura Y, Li MY, Birey F, Ikeda K, Revah O, Thete MV, Park JY, Puno A, Lee SH, Porteus MH, Pașca SP, et al.
Nature biotechnology (2020) 3812: 1421-1430. 214 011 IHC; tested species: human

Deletion of class II ARFs in mice causes tremor by the Nav1.6 loss in cerebellar Purkinje cell axon initial segments.
Hosoi N, Shibasaki K, Hosono M, Konno A, Shinoda Y, Kiyonari H, Inoue K, Muramatsu SI, Ishizaki Y, Hirai H, Furuichi T, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) : . 214 011 IHC; tested species: mouse

Heterogeneity of GRIM-19 Expression in the Adult Mouse Brain.
Hwang SN, Kim JC, Kim SY
Cellular and molecular neurobiology (2019) : . 214 011 IHC; tested species: mouse

Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody.
Kaczmarek-Hajek K, Zhang J, Kopp R, Grosche A, Rissiek B, Saul A, Bruzzone S, Engel T, Jooss T, Krautloher A, Schuster S, et al.
eLife (2018) 7: . 214 011 IHC; tested species: mouse

Facilitation of Contextual Fear Extinction by Orexin-1 Receptor Antagonism Is Associated with the Activation of Specific Amygdala Cell Subpopulations.
Flores Á, Herry C, Maldonado R, Berrendero F
The international journal of neuropsychopharmacology (2017) 208: 654-659. 214 011 IHC; tested species: mouse

Functional expression of calcium-permeable canonical transient receptor potential 4-containing channels promotes migration of medulloblastoma cells.
Wei WC, Huang WC, Lin YP, Becker EBE, Ansorge O, Flockerzi V, Conti D, Cenacchi G, Glitsch MD
The Journal of physiology (2017) 59516: 5525-5544. 214 011 IHC; tested species: mouse

Characterization of a novel subtype of hippocampal interneurons that express corticotropin-releasing hormone.
Hooper A, Maguire J
Hippocampus (2016) 261: 41-53. 214 011 IHC

Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse.
Fuchs M, Brandstätter JH, Regus-Leidig H
Frontiers in cellular neuroscience (2014) 8: 60. 214 011 IHC; tested species: rat

Rescue of bilirubin-induced neonatal lethality in a mouse model of Crigler-Najjar syndrome type I by AAV9-mediated gene transfer.
Bortolussi G, Zentilin L, Baj G, Giraudi P, Bellarosa C, Giacca M, Tiribelli C, Muro AF
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2012) 263: 1052-63. 214 011 IHC; tested species: mouse

Probing the functional equivalence of otoferlin and synaptotagmin 1 in exocytosis.
Reisinger E, Bresee C, Neef J, Nair R, Reuter K, Bulankina A, Nouvian R, Koch M, Bückers J, Kastrup L, Roux I, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3113: 4886-95. 214 011 IHC; tested species: mouse

CLARITY increases sensitivity and specificity of fluorescence immunostaining in long-term archived human brain tissue.
Woelfle S, Deshpande D, Feldengut S, Braak H, Del Tredici K, Roselli F, Deisseroth K, Michaelis J, Boeckers TM, Schön M
BMC biology (2023) 211: 113. 214 011 Clarity; tested species: human

Expression profiles of the autism-related SHANK proteins in the human brain.
Woelfle S, Pedro MT, Wagner J, Schön M, Boeckers TM
BMC biology (2023) 211: 254. 214 011 Clarity; tested species: human

Inhibition of West Nile virus by calbindin-D28k.
Siddharthan V, Wang H, Davies CJ, Hall JO, Morrey JD
PloS one (2014) 99: e106535. 214 011 FACS

Cat. No.: 214 011

Amount: 100 µg

Price: $425.00

References
WB (2)
IHC (19)
Clarity (2)
FACS (1)

Heterogeneity of GRIM-19 Expression in the Adult Mouse Brain.
Hwang SN, Kim JC, Kim SY
Cellular and molecular neurobiology (2019) : . 214 011 IHC; tested species: mouse

Characterization of a novel subtype of hippocampal interneurons that express corticotropin-releasing hormone.
Hooper A, Maguire J
Hippocampus (2016) 261: 41-53. 214 011 IHC

Inhibition of West Nile virus by calbindin-D28k.
Siddharthan V, Wang H, Davies CJ, Hall JO, Morrey JD
PloS one (2014) 99: e106535. 214 011 FACS

ICC

IHC

IHC-P

Background

Calbindin D28k (CALB1, D28K or CAB27) and Calretinin (Calbindin D29k) are members of the EF-hand calcium-binding protein family, playing essential roles in intracellular calcium homeostasis, neuroprotection, and signal transduction (1). Calbindin D28k is widely expressed in tissues that experience high calcium influx, such as the brain, sensory organs, endocrine tissues, and the enteric nervous system (ENS). In the central nervous system (CNS), Calbindin D28k is an important marker for specific neuronal populations, particularly in Purkinje cells of the cerebellum and GABAergic and glutamatergic neurons of the cortex (2). Outside the CNS, Calbindin D28k plays crucial roles in non-neuronal tissues. In the retina, it contributes to photoreceptor function and calcium signaling (3). In the pancreas, it is present in α- and β-cells (4). Additionally, it was reported that 31% of myenteric neurons and up to 95% of submucosal neurons in the human ENS express calbindin, suggesting its involvement in both motor and secretory functions (5).

1 ) Schwaller B et al., Cerebellum (2002)
2 ) Flace P et al., Anat Rec (Hoboken) (2014)
3 ) Gu YN et al., Exp Eye Res (2016)
4 ) Janssen SW et al., J Histochem Cytochem (2002)
5 ) Zetzmann K et al., Int J Mol Sci (2018)

Protocols

AP Detection
ECL Detection
Fluorescent detection
Pre-adsorption protocol

Standard protocol

ICC

PFA fixation
Pre-adsorption protocol

IHC

Free floating
Slide mounted
Tissue preparation
Pre-adsorption protocol

IHC-P

Chromogenic detection
Fluorescent detection
Tissue preparation
Pre-adsorption protocol

OTHER

Preabsorbtion

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Calbindin D28k antibody - 214 011

References for Calbindin D28k - 214 011