Synaptic Systems - Calbindin D28k

Cat. No.: 214 004

Amount: 100 µl

Price: $400.00

Cat. No. 214 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: 1 : 200 gallery
Immunogen	Full-length recombinant human Calbindin D28k protein (UniProt Id: P05937)
Reactivity	Reacts with: human (P05937), rat (P07171), mouse (P12658), monkey, ape, cow. Other species not tested yet.
Matching control protein/peptide	214-0P
Data sheet	214_004.pdf

References for Calbindin D28k - 214 004

WB (1)
ICC (3)
IHC (25)
IHC-P (1)

Non-canonical function of ADAM10 in presynaptic plasticity.
Bär J, Fanutza T, Reimann CC, Seipold L, Grohe M, Bolter JR, Delfs F, Bucher M, Gee CE, Schweizer M, Saftig P, et al.
Cellular and molecular life sciences : CMLS (2024) 811: 342. 214 004 WB; tested species: mouse

Myosin 18Aα targets the guanine nucleotide exchange factor β-Pix to the dendritic spines of cerebellar Purkinje neurons and promotes spine maturation.
Alexander CJ, Barzik M, Fujiwara I, Remmert K, Wang YX, Petralia RS, Friedman TB, Hammer JA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2021) 351: e21092. 214 004 ICC; tested species: mouse

The Bacterial Enzyme RfxCas13d Is Less Neurotoxic Than PspCas13b and Could Be a Promising RNA Editing and Interference Tool in the Nervous System.
Wu QW, Kapfhammer JP
Brain sciences (2021) 118: . 214 004 ICC; tested species: mouse

Experimental Epileptogenesis in a Cell Culture Model of Primary Neurons from Rat Brain: A Temporal Multi-Scale Study.
Jablonski J, Hoffmann L, Blümcke I, Fejtová A, Uebe S, Ekici AB, Gnatkovsky V, Kobow K
Cells (2021) 1011: . 214 004 ICC; tested species: rat

The Burning Pain Transcriptome in the Mouse Primary Somatosensory Cortex.
Erdei V, Mészár Z, Varga A
International journal of molecular sciences (2025) 268: . 214 004 IHC; tested species: mouse

Overexpression of the autism candidate gene Cyfip1 pathologically enhances olivo-cerebellar signaling in mice.
Busch SE, Simmons DH, Gama E, Du X, Longo F, Gomez CM, Klann E, Hansel C
Frontiers in cellular neuroscience (2023) 17: 1219270. 214 004 IHC; tested species: mouse

Selective induction of human renal interstitial progenitor-like cell lineages from iPSCs reveals development of mesangial and EPO-producing cells.
Tsujimoto H, Hoshina A, Mae SI, Araoka T, Changting W, Ijiri Y, Nakajima-Koyama M, Sakurai S, Okita K, Mizuta K, Niwa A, et al.
Cell reports (2023) : 113602. 214 004 IHC; tested species: mouse

Decreasing mutant ATXN1 nuclear localization improves a spectrum of SCA1-like phenotypes and brain region transcriptomic profiles.
Handler HP, Duvick L, Mitchell JS, Cvetanovic M, Reighard M, Soles A, Mather KB, Rainwater O, Serres S, Nichols-Meade T, Coffin SL, et al.
Neuron (2022) : . 214 004 IHC; tested species: mouse

Homeostatic control of nuclear-encoded mitochondrial gene expression by the histone variant H2A.Z is essential for neuronal survival.
Lowden C, Boulet A, Boehler NA, Seecharran S, Rios Garcia J, Lowe NJ, Liu J, Ong JLK, Wang W, Ma L, Cheng AH, et al.
Cell reports (2021) 3611: 109704. 214 004 IHC; tested species: mouse

Total Number and Ratio of GABAergic Neuron Types in the Mouse Lateral and Basal Amygdala.
Vereczki VK, Müller K, Krizsán É, Máté Z, Fekete Z, Rovira-Esteban L, Veres JM, Erdélyi F, Hájos N
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) 4121: 4575-4595. 214 004 IHC; tested species: mouse

TTBK2 and primary cilia are essential for the connectivity and survival of cerebellar Purkinje neurons.
Bowie E, Goetz SC
eLife (2020) 9: . 214 004 IHC; tested species: mouse

A Modular Differentiation System Maps Multiple Human Kidney Lineages from Pluripotent Stem Cells.
Tsujimoto H, Kasahara T, Sueta SI, Araoka T, Sakamoto S, Okada C, Mae SI, Nakajima T, Okamoto N, Taura D, Nasu M, et al.
Cell reports (2020) 311: 107476. 214 004 IHC; tested species: mouse

Defining the Vulnerability Window of Anesthesia-Induced Neuroapoptosis in Developing Dentate Gyrus Granule Cells - A Transgenic Approach Utilizing POMC-EGFP Mice.
Wei K, Chen P, Shen FY, Zhang Y, Liu YH, Wang ZR, Loepke AW, Wang YW, Deng M
Neuroscience (2019) : . 214 004 IHC; tested species: mouse

Non-canonical heterogeneous cellular distribution and co-localization of CaMKIIα and CaMKIIβ in the spinal superficial dorsal horn.
Larsson M
Brain structure & function (2018) 2233: 1437-1457. 214 004 IHC; tested species: rat

Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability upon acute stress.
Alpár A, Zahola P, Hanics J, Hevesi Z, Korchynska S, Benevento M, Pifl C, Zachar G, Perugini J, Severi I, Leitgeb P, et al.
The EMBO journal (2018) : . 214 004 IHC; tested species: mouse

Timely regulated sorting from early to late endosomes is required to maintain cerebellar long-term depression.
Kim T, Yamamoto Y, Tanaka-Yamamoto K
Nature communications (2017) 81: 401. 214 004 IHC; tested species: mouse

Abolished perineuronal nets and altered parvalbumin-immunoreactivity in the nucleus reticularis thalami of wildtype and 3xTg mice after experimental stroke.
Härtig W, Appel S, Suttkus A, Grosche J, Michalski D
Neuroscience (2016) 337: 66-87. 214 004 IHC

Development of early-born γ-Aminobutyric acid hub neurons in mouse hippocampus from embryogenesis to adulthood.
Villette V, Guigue P, Picardo MA, Sousa VH, Leprince E, Lachamp P, Malvache A, Tressard T, Cossart R, Baude A
The Journal of comparative neurology (2016) 52412: 2440-61. 214 004 IHC; tested species: mouse

Prolonged Type 1 Metabotropic Glutamate Receptor Dependent Synaptic Signaling Contributes to Spino-Cerebellar Ataxia Type 1.
Power EM, Morales A, Empson RM
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 3618: 4910-6. 214 004 IHC; tested species: mouse

Synaptic Organization of Perisomatic GABAergic Inputs onto the Principal Cells of the Mouse Basolateral Amygdala.
Vereczki VK, Veres JM, Müller K, Nagy GA, Rácz B, Barsy B, Hájos N
Frontiers in neuroanatomy (2016) 10: 20. 214 004 IHC; tested species: mouse

High Resolution Dissection of Reactive Glial Nets in Alzheimer's Disease.
Bouvier DS, Jones EV, Quesseveur G, Davoli MA, A Ferreira T, Quirion R, Mechawar N, Murai KK
Scientific reports (2016) 6: 24544. 214 004 IHC; tested species: human

Functional and Physical Interaction of Diacylglycerol Kinase ζ with Protein Kinase Cα Is Required for Cerebellar Long-Term Depression.
Lee D, Yamamoto Y, Kim E, Tanaka-Yamamoto K
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3546: 15453-65. 214 004 IHC

An excitatory GABA loop operating in vivo.
Astorga G, Bao J, Marty A, Augustine GJ, Franconville R, Jalil A, Bradley J, Llano I
Frontiers in cellular neuroscience (2015) 9: 275. 214 004 IHC

Cell age-specific vulnerability of neurons to anesthetic toxicity.
Hofacer RD, Deng M, Ward CG, Joseph B, Hughes EA, Jiang C, Danzer SC, Loepke AW
Annals of neurology (2013) 736: 695-704. 214 004 IHC

Raf kinase inhibitory protein is required for cerebellar long-term synaptic depression by mediating PKC-dependent MAPK activation.
Yamamoto Y, Lee D, Kim Y, Lee B, Seo C, Kawasaki H, Kuroda S, Tanaka-Yamamoto K
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3241: 14254-64. 214 004 IHC

Spontaneous activity promotes synapse formation in a cell-type-dependent manner in the developing retina.
Soto F, Ma X, Cecil JL, Vo BQ, Culican SM, Kerschensteiner D
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3216: 5426-39. 214 004 IHC

Postmitotic neurons develop a p21-dependent senescence-like phenotype driven by a DNA damage response.
Jurk D, Wang C, Miwa S, Maddick M, Korolchuk V, Tsolou A, Gonos ES, Thrasivoulou C, Saffrey MJ, Cameron K, von Zglinicki T, et al.
Aging cell (2012) 116: 996-1004. 214 004 IHC

Slow age-dependent decline of doublecortin expression and BrdU labeling in the forebrain from lesser hedgehog tenrecs.
Alpár A, Künzle H, Gärtner U, Popkova Y, Bauer U, Grosche J, Reichenbach A, Härtig W
Brain research (2010) 1330: 9-19. 214 004 IHC

Excitation of Diverse Classes of Cholecystokinin Interneurons in the Basal Amygdala Facilitates Fear Extinction.
Rovira-Esteban L, Gunduz-Cinar O, Bukalo O, Limoges A, Brockway E, Müller K, Fenno L, Kim YS, Ramakrishnan C, Andrási T, Deisseroth K, et al.
eNeuro () 66: . 214 004 IHC; tested species: mouse

Single cell RNA sequencing uncovers cellular developmental sequences and novel potential intercellular communications in embryonic kidney.
Matsui I, Matsumoto A, Inoue K, Katsuma Y, Yasuda S, Shimada K, Sakaguchi Y, Mizui M, Kaimori JY, Takabatake Y, Isaka Y, et al.
Scientific reports (2021) 111: 73. 214 004 IHC-P; tested species: mouse

Cat. No.: 214 004

Amount: 100 µl

Price: $400.00

References
WB (1)
ICC (3)
IHC (25)
IHC-P (1)

The Burning Pain Transcriptome in the Mouse Primary Somatosensory Cortex.
Erdei V, Mészár Z, Varga A
International journal of molecular sciences (2025) 268: . 214 004 IHC; tested species: mouse

TTBK2 and primary cilia are essential for the connectivity and survival of cerebellar Purkinje neurons.
Bowie E, Goetz SC
eLife (2020) 9: . 214 004 IHC; tested species: mouse

An excitatory GABA loop operating in vivo.
Astorga G, Bao J, Marty A, Augustine GJ, Franconville R, Jalil A, Bradley J, Llano I
Frontiers in cellular neuroscience (2015) 9: 275. 214 004 IHC

ICC

IHC

IHC-P

Background

Calbindin D28k (CALB1, D28K or CAB27) and Calretinin (Calbindin D29k) are members of the EF-hand calcium-binding protein family, playing essential roles in intracellular calcium homeostasis, neuroprotection, and signal transduction (1). Calbindin D28k is widely expressed in tissues that experience high calcium influx, such as the brain, sensory organs, endocrine tissues, and the enteric nervous system (ENS). In the central nervous system (CNS), Calbindin D28k is an important marker for specific neuronal populations, particularly in Purkinje cells of the cerebellum and GABAergic and glutamatergic neurons of the cortex (2). Outside the CNS, Calbindin D28k plays crucial roles in non-neuronal tissues. In the retina, it contributes to photoreceptor function and calcium signaling (3). In the pancreas, it is present in α- and β-cells (4). Additionally, it was reported that 31% of myenteric neurons and up to 95% of submucosal neurons in the human ENS express calbindin, suggesting its involvement in both motor and secretory functions (5).

1 ) Schwaller B et al., Cerebellum (2002)
2 ) Flace P et al., Anat Rec (Hoboken) (2014)
3 ) Gu YN et al., Exp Eye Res (2016)
4 ) Janssen SW et al., J Histochem Cytochem (2002)
5 ) Zetzmann K et al., Int J Mol Sci (2018)

Protocols

AP Detection
ECL Detection
Fluorescent detection
Pre-adsorption protocol

Standard protocol

ICC

PFA fixation
Pre-adsorption protocol

IHC

Free floating
Slide mounted
Tissue preparation
Pre-adsorption protocol

IHC-P

Chromogenic detection
Fluorescent detection
Tissue preparation
Pre-adsorption protocol

OTHER

Preabsorbtion

Other products for this target

Calbindin D28k antibody - 214 004

References for Calbindin D28k - 214 004