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Calbindin D28k - 214 004

A vitamin D-dependent calcium binding protein
Polyclonal Guinea pig antiserum
Cat. No.: 214 004
Amount: 100 µl
Price: $375.00
Cat. No. 214 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P/FFPE: 1 : 200 gallery  
Immunogen Recombinant protein corresponding to AA 3 to 251 from human CalbindinD28k (UniProt Id: P05937)
Reactivity Reacts with: human (P05937), rat (P07171), mouse (P12658), monkey, ape, cow.
Other species not tested yet.
Specificity Specific for calbindin D28k.
Matching control protein/peptide 214-0P
Data sheet 214_004.pdf

References for Calbindin D28k - 214 004

Myosin 18Aα targets the guanine nucleotide exchange factor β-Pix to the dendritic spines of cerebellar Purkinje neurons and promotes spine maturation.
Alexander CJ, Barzik M, Fujiwara I, Remmert K, Wang YX, Petralia RS, Friedman TB, Hammer JA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2021) 351: e21092. 214 004 ICC; tested species: mouse
A Modular Differentiation System Maps Multiple Human Kidney Lineages from Pluripotent Stem Cells.
Tsujimoto H, Kasahara T, Sueta SI, Araoka T, Sakamoto S, Okada C, Mae SI, Nakajima T, Okamoto N, Taura D, Nasu M, et al.
Cell reports (2020) 311: 107476. 214 004 IHC; tested species: mouse
TTBK2 and primary cilia are essential for the connectivity and survival of cerebellar Purkinje neurons.
Bowie E, Goetz SC
eLife (2020) 9: . 214 004 IHC; tested species: mouse
Defining the Vulnerability Window of Anesthesia-Induced Neuroapoptosis in Developing Dentate Gyrus Granule Cells - A Transgenic Approach Utilizing POMC-EGFP Mice.
Wei K, Chen P, Shen FY, Zhang Y, Liu YH, Wang ZR, Loepke AW, Wang YW, Deng M
Neuroscience (2019) : . 214 004 IHC; tested species: mouse
Non-canonical heterogeneous cellular distribution and co-localization of CaMKIIα and CaMKIIβ in the spinal superficial dorsal horn.
Larsson M
Brain structure & function (2018) 2233: 1437-1457. 214 004 IHC; tested species: rat
Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability upon acute stress.
Alpár A, Zahola P, Hanics J, Hevesi Z, Korchynska S, Benevento M, Pifl C, Zachar G, Perugini J, Severi I, Leitgeb P, et al.
The EMBO journal (2018) : . 214 004 IHC; tested species: mouse
Timely regulated sorting from early to late endosomes is required to maintain cerebellar long-term depression.
Kim T, Yamamoto Y, Tanaka-Yamamoto K
Nature communications (2017) 81: 401. 214 004 IHC; tested species: mouse
Abolished perineuronal nets and altered parvalbumin-immunoreactivity in the nucleus reticularis thalami of wildtype and 3xTg mice after experimental stroke.
Härtig W, Appel S, Suttkus A, Grosche J, Michalski D
Neuroscience (2016) 337: 66-87. 214 004 IHC
Development of early-born γ-Aminobutyric acid hub neurons in mouse hippocampus from embryogenesis to adulthood.
Villette V, Guigue P, Picardo MA, Sousa VH, Leprince E, Lachamp P, Malvache A, Tressard T, Cossart R, Baude A
The Journal of comparative neurology (2016) 52412: 2440-61. 214 004 IHC; tested species: mouse
Prolonged Type 1 Metabotropic Glutamate Receptor Dependent Synaptic Signaling Contributes to Spino-Cerebellar Ataxia Type 1.
Power EM, Morales A, Empson RM
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 3618: 4910-6. 214 004 IHC; tested species: mouse
Synaptic Organization of Perisomatic GABAergic Inputs onto the Principal Cells of the Mouse Basolateral Amygdala.
Vereczki VK, Veres JM, Müller K, Nagy GA, Rácz B, Barsy B, Hájos N
Frontiers in neuroanatomy (2016) 10: 20. 214 004 IHC; tested species: mouse
High Resolution Dissection of Reactive Glial Nets in Alzheimer's Disease.
Bouvier DS, Jones EV, Quesseveur G, Davoli MA, A Ferreira T, Quirion R, Mechawar N, Murai KK
Scientific reports (2016) 6: 24544. 214 004 IHC; tested species: human
An excitatory GABA loop operating in vivo.
Astorga G, Bao J, Marty A, Augustine GJ, Franconville R, Jalil A, Bradley J, Llano I
Frontiers in cellular neuroscience (2015) 9: 275. 214 004 IHC
Functional and Physical Interaction of Diacylglycerol Kinase ζ with Protein Kinase Cα Is Required for Cerebellar Long-Term Depression.
Lee D, Yamamoto Y, Kim E, Tanaka-Yamamoto K
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3546: 15453-65. 214 004 IHC
Cell age-specific vulnerability of neurons to anesthetic toxicity.
Hofacer RD, Deng M, Ward CG, Joseph B, Hughes EA, Jiang C, Danzer SC, Loepke AW
Annals of neurology (2013) 736: 695-704. 214 004 IHC
Raf kinase inhibitory protein is required for cerebellar long-term synaptic depression by mediating PKC-dependent MAPK activation.
Yamamoto Y, Lee D, Kim Y, Lee B, Seo C, Kawasaki H, Kuroda S, Tanaka-Yamamoto K
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3241: 14254-64. 214 004 IHC
Spontaneous activity promotes synapse formation in a cell-type-dependent manner in the developing retina.
Soto F, Ma X, Cecil JL, Vo BQ, Culican SM, Kerschensteiner D
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3216: 5426-39. 214 004 IHC
Postmitotic neurons develop a p21-dependent senescence-like phenotype driven by a DNA damage response.
Jurk D, Wang C, Miwa S, Maddick M, Korolchuk V, Tsolou A, Gonos ES, Thrasivoulou C, Saffrey MJ, Cameron K, von Zglinicki T, et al.
Aging cell (2012) 116: 996-1004. 214 004 IHC
Slow age-dependent decline of doublecortin expression and BrdU labeling in the forebrain from lesser hedgehog tenrecs.
Alpár A, Künzle H, Gärtner U, Popkova Y, Bauer U, Grosche J, Reichenbach A, Härtig W
Brain research (2010) 1330: 9-19. 214 004 IHC
Excitation of Diverse Classes of Cholecystokinin Interneurons in the Basal Amygdala Facilitates Fear Extinction.
Rovira-Esteban L, Gunduz-Cinar O, Bukalo O, Limoges A, Brockway E, Müller K, Fenno L, Kim YS, Ramakrishnan C, Andrási T, Deisseroth K, et al.
eNeuro () 66: . 214 004 IHC; tested species: mouse
Single cell RNA sequencing uncovers cellular developmental sequences and novel potential intercellular communications in embryonic kidney.
Matsui I, Matsumoto A, Inoue K, Katsuma Y, Yasuda S, Shimada K, Sakaguchi Y, Mizui M, Kaimori JY, Takabatake Y, Isaka Y, et al.
Scientific reports (2021) 111: 73. 214 004 IHC-P; tested species: mouse
Cat. No.: 214 004
Quantity: 100 µl
Price: $375.00
Myosin 18Aα targets the guanine nucleotide exchange factor β-Pix to the dendritic spines of cerebellar Purkinje neurons and promotes spine maturation.
Alexander CJ, Barzik M, Fujiwara I, Remmert K, Wang YX, Petralia RS, Friedman TB, Hammer JA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2021) 351: e21092. 214 004 ICC; tested species: mouse
A Modular Differentiation System Maps Multiple Human Kidney Lineages from Pluripotent Stem Cells.
Tsujimoto H, Kasahara T, Sueta SI, Araoka T, Sakamoto S, Okada C, Mae SI, Nakajima T, Okamoto N, Taura D, Nasu M, et al.
Cell reports (2020) 311: 107476. 214 004 IHC; tested species: mouse
TTBK2 and primary cilia are essential for the connectivity and survival of cerebellar Purkinje neurons.
Bowie E, Goetz SC
eLife (2020) 9: . 214 004 IHC; tested species: mouse
Defining the Vulnerability Window of Anesthesia-Induced Neuroapoptosis in Developing Dentate Gyrus Granule Cells - A Transgenic Approach Utilizing POMC-EGFP Mice.
Wei K, Chen P, Shen FY, Zhang Y, Liu YH, Wang ZR, Loepke AW, Wang YW, Deng M
Neuroscience (2019) : . 214 004 IHC; tested species: mouse
Non-canonical heterogeneous cellular distribution and co-localization of CaMKIIα and CaMKIIβ in the spinal superficial dorsal horn.
Larsson M
Brain structure & function (2018) 2233: 1437-1457. 214 004 IHC; tested species: rat
Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability upon acute stress.
Alpár A, Zahola P, Hanics J, Hevesi Z, Korchynska S, Benevento M, Pifl C, Zachar G, Perugini J, Severi I, Leitgeb P, et al.
The EMBO journal (2018) : . 214 004 IHC; tested species: mouse
Timely regulated sorting from early to late endosomes is required to maintain cerebellar long-term depression.
Kim T, Yamamoto Y, Tanaka-Yamamoto K
Nature communications (2017) 81: 401. 214 004 IHC; tested species: mouse
Abolished perineuronal nets and altered parvalbumin-immunoreactivity in the nucleus reticularis thalami of wildtype and 3xTg mice after experimental stroke.
Härtig W, Appel S, Suttkus A, Grosche J, Michalski D
Neuroscience (2016) 337: 66-87. 214 004 IHC
Development of early-born γ-Aminobutyric acid hub neurons in mouse hippocampus from embryogenesis to adulthood.
Villette V, Guigue P, Picardo MA, Sousa VH, Leprince E, Lachamp P, Malvache A, Tressard T, Cossart R, Baude A
The Journal of comparative neurology (2016) 52412: 2440-61. 214 004 IHC; tested species: mouse
Prolonged Type 1 Metabotropic Glutamate Receptor Dependent Synaptic Signaling Contributes to Spino-Cerebellar Ataxia Type 1.
Power EM, Morales A, Empson RM
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 3618: 4910-6. 214 004 IHC; tested species: mouse
Synaptic Organization of Perisomatic GABAergic Inputs onto the Principal Cells of the Mouse Basolateral Amygdala.
Vereczki VK, Veres JM, Müller K, Nagy GA, Rácz B, Barsy B, Hájos N
Frontiers in neuroanatomy (2016) 10: 20. 214 004 IHC; tested species: mouse
High Resolution Dissection of Reactive Glial Nets in Alzheimer's Disease.
Bouvier DS, Jones EV, Quesseveur G, Davoli MA, A Ferreira T, Quirion R, Mechawar N, Murai KK
Scientific reports (2016) 6: 24544. 214 004 IHC; tested species: human
An excitatory GABA loop operating in vivo.
Astorga G, Bao J, Marty A, Augustine GJ, Franconville R, Jalil A, Bradley J, Llano I
Frontiers in cellular neuroscience (2015) 9: 275. 214 004 IHC
Functional and Physical Interaction of Diacylglycerol Kinase ζ with Protein Kinase Cα Is Required for Cerebellar Long-Term Depression.
Lee D, Yamamoto Y, Kim E, Tanaka-Yamamoto K
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3546: 15453-65. 214 004 IHC
Cell age-specific vulnerability of neurons to anesthetic toxicity.
Hofacer RD, Deng M, Ward CG, Joseph B, Hughes EA, Jiang C, Danzer SC, Loepke AW
Annals of neurology (2013) 736: 695-704. 214 004 IHC
Raf kinase inhibitory protein is required for cerebellar long-term synaptic depression by mediating PKC-dependent MAPK activation.
Yamamoto Y, Lee D, Kim Y, Lee B, Seo C, Kawasaki H, Kuroda S, Tanaka-Yamamoto K
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3241: 14254-64. 214 004 IHC
Spontaneous activity promotes synapse formation in a cell-type-dependent manner in the developing retina.
Soto F, Ma X, Cecil JL, Vo BQ, Culican SM, Kerschensteiner D
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3216: 5426-39. 214 004 IHC
Postmitotic neurons develop a p21-dependent senescence-like phenotype driven by a DNA damage response.
Jurk D, Wang C, Miwa S, Maddick M, Korolchuk V, Tsolou A, Gonos ES, Thrasivoulou C, Saffrey MJ, Cameron K, von Zglinicki T, et al.
Aging cell (2012) 116: 996-1004. 214 004 IHC
Slow age-dependent decline of doublecortin expression and BrdU labeling in the forebrain from lesser hedgehog tenrecs.
Alpár A, Künzle H, Gärtner U, Popkova Y, Bauer U, Grosche J, Reichenbach A, Härtig W
Brain research (2010) 1330: 9-19. 214 004 IHC
Excitation of Diverse Classes of Cholecystokinin Interneurons in the Basal Amygdala Facilitates Fear Extinction.
Rovira-Esteban L, Gunduz-Cinar O, Bukalo O, Limoges A, Brockway E, Müller K, Fenno L, Kim YS, Ramakrishnan C, Andrási T, Deisseroth K, et al.
eNeuro () 66: . 214 004 IHC; tested species: mouse
Single cell RNA sequencing uncovers cellular developmental sequences and novel potential intercellular communications in embryonic kidney.
Matsui I, Matsumoto A, Inoue K, Katsuma Y, Yasuda S, Shimada K, Sakaguchi Y, Mizui M, Kaimori JY, Takabatake Y, Isaka Y, et al.
Scientific reports (2021) 111: 73. 214 004 IHC-P; tested species: mouse
Background

Two isoforms of the vitamin D-dependent Ca-binding proteins have been described so far: calbindin D28k, also referred to as CALB 1, D-28k, and CAB 27, and calbindin D29k, also known as calretinin. These proteins are expressed in cells that have to handle a high calcium influx such as brain, bone, teeth, inner ear and others. Calbindins are believed to regulate cellular activity by suppressing or buffering intracellur calcium. In the brain calbindin D28k is a useful marker for specific neuronal cell types. It is particularly concentrated in the dendrites and perikarya of cerebellar Purkinje cells, but is also found in many GABAergic interneurons in the cortex.