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Calbindin D28k antibody - 214 009

Calbindin D28k is a vitamin D-dependent calcium binding protein
Chicken monoclonal recombinant IgY
Cat. No.: 214 009
Amount: 50 µg
Price: $415.00
Cat. No. 214 009 50 µg purified recombinant IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 4000 (AP-staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 2000 gallery  
IHC-P: 1 : 2000 gallery  
Clone Ch351C10
Subtype IgY (λ light chain)
Immunogen Full-length recombinant human Calbindin D28k protein (UniProt Id: P05937)
Reactivity Reacts with: human (P05937), rat (P07171), mouse (P12658), zebrafish, grashopper.
Other species not tested yet.
Matching control protein/peptide 214-0P
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody 351C10. The constant regions of the heavy and light chains have been replaced by chicken specific sequences. The antibody can therefore be used with standard anti-chicken secondary reagents. The antibody has been expressed in mammalian cells.

Data sheet 214_009.pdf
Cat. No.: 214 009
Amount: 50 µg
Price: $415.00
Background

Two isoforms of the vitamin D-dependent Ca-binding proteins have been described so far: calbindin D28k, also referred to as CALB 1, D-28k, and CAB 27, and calbindin D29k, also known as calretinin. These proteins are expressed in cells that have to handle a high calcium influx such as brain, bone, teeth, inner ear and others. Calbindins are believed to regulate cellular activity by suppressing or buffering intracellur calcium. In the brain calbindin D28k is a useful marker for specific neuronal cell types. It is particularly concentrated in the dendrites and perikarya of cerebellar Purkinje cells, but is also found in many GABAergic interneurons in the cortex.