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Calbindin D28k - 214 002

A vitamin D-dependent calcium binding protein
Polyclonal rabbit antiserum
Cat. No.: 214 002
Amount: 200 µl
Price: $350.00
Cat. No. 214 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: yes
ICC: 1 : 5000 gallery  
IHC: 1 : 500 gallery  
IHC-P/FFPE: 1 : 200 gallery  
Immunogen Recombinant protein corresponding to AA 3 to 251 from human CalbindinD28k (UniProt Id: P05937)
Reactivity Reacts with: human (P05937), rat (P07171), mouse (P12658), monkey, ape, cow, chicken, zebrafish, turtle, grashopper.
Other species not tested yet.
Specificity Specific for calbindin D28k.
Matching control protein/peptide 214-0P
Data sheet 214_002.pdf

References for Calbindin D28k - 214 002

Polylaminin recognition by retinal cells.
Hochman-Mendez C, Lacerda de Menezes JR, Sholl-Franco A, Coelho-Sampaio T
Journal of neuroscience research (2014) 921: 24-34. 214 002 ICC; tested species: rat
Loss of Piccolo Function in Rats Induces Cerebellar Network Dysfunction and Pontocerebellar Hypoplasia Type 3-like Phenotypes.
Falck J, Bruns C, Hoffmann-Conaway S, Straub I, Plautz EJ, Orlando M, Munawar H, Rivalan M, Winter Y, Izsvák Z, Schmitz D, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4014: 2943-2959. 214 002 IHC; tested species: rat
Permanent Whisker Removal Reduces the Density of c-Fos+ Cells and the Expression of Calbindin Protein, Disrupts Hippocampal Neurogenesis and Affects Spatial-Memory-Related Tasks.
Gonzalez-Perez O, López-Virgen V, Ibarra-Castaneda N
Frontiers in cellular neuroscience (2018) 12: 132. 214 002 IHC; tested species: mouse
Pharmacological targeting of GSK-3 and NRF2 provides neuroprotection in a preclinical model of tauopathy.
Cuadrado A, Kügler S, Lastres-Becker I
Redox biology (2018) 14: 522-534. 214 002 IHC; tested species: mouse
Abolished perineuronal nets and altered parvalbumin-immunoreactivity in the nucleus reticularis thalami of wildtype and 3xTg mice after experimental stroke.
Härtig W, Appel S, Suttkus A, Grosche J, Michalski D
Neuroscience (2016) 337: 66-87. 214 002 IHC
NGL-2 regulates pathway-specific neurite growth and lamination, synapse formation, and signal transmission in the retina.
Soto F, Watkins KL, Johnson RE, Schottler F, Kerschensteiner D
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3329: 11949-59. 214 002 IHC
Chronic mild stress modulates 5-HT1A and 5-HT2A receptor expression in the cerebellar cortex of NC/Nga atopic-like mice.
Rasul A, Johansson B, Lonne-Rahm SB, Nordlind K, Theodorsson E, El-Nour H
Archives of dermatological research (2013) 3055: 407-13. 214 002 IHC
Probing the functional equivalence of otoferlin and synaptotagmin 1 in exocytosis.
Reisinger E, Bresee C, Neef J, Nair R, Reuter K, Bulankina A, Nouvian R, Koch M, Bückers J, Kastrup L, Roux I, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3113: 4886-95. 214 002 IHC; tested species: mouse
Characterization of Tissue Plasminogen Activator Expression and Trafficking in the Adult Murine Brain.
Stevenson TK, Lawrence DA
eNeuro () 54: . 214 002 IHC; tested species: mouse
Cat. No.: 214 002
Quantity: 200 µl
Price: $350.00
Polylaminin recognition by retinal cells.
Hochman-Mendez C, Lacerda de Menezes JR, Sholl-Franco A, Coelho-Sampaio T
Journal of neuroscience research (2014) 921: 24-34. 214 002 ICC; tested species: rat
Loss of Piccolo Function in Rats Induces Cerebellar Network Dysfunction and Pontocerebellar Hypoplasia Type 3-like Phenotypes.
Falck J, Bruns C, Hoffmann-Conaway S, Straub I, Plautz EJ, Orlando M, Munawar H, Rivalan M, Winter Y, Izsvák Z, Schmitz D, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4014: 2943-2959. 214 002 IHC; tested species: rat
Permanent Whisker Removal Reduces the Density of c-Fos+ Cells and the Expression of Calbindin Protein, Disrupts Hippocampal Neurogenesis and Affects Spatial-Memory-Related Tasks.
Gonzalez-Perez O, López-Virgen V, Ibarra-Castaneda N
Frontiers in cellular neuroscience (2018) 12: 132. 214 002 IHC; tested species: mouse
Pharmacological targeting of GSK-3 and NRF2 provides neuroprotection in a preclinical model of tauopathy.
Cuadrado A, Kügler S, Lastres-Becker I
Redox biology (2018) 14: 522-534. 214 002 IHC; tested species: mouse
Abolished perineuronal nets and altered parvalbumin-immunoreactivity in the nucleus reticularis thalami of wildtype and 3xTg mice after experimental stroke.
Härtig W, Appel S, Suttkus A, Grosche J, Michalski D
Neuroscience (2016) 337: 66-87. 214 002 IHC
NGL-2 regulates pathway-specific neurite growth and lamination, synapse formation, and signal transmission in the retina.
Soto F, Watkins KL, Johnson RE, Schottler F, Kerschensteiner D
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3329: 11949-59. 214 002 IHC
Chronic mild stress modulates 5-HT1A and 5-HT2A receptor expression in the cerebellar cortex of NC/Nga atopic-like mice.
Rasul A, Johansson B, Lonne-Rahm SB, Nordlind K, Theodorsson E, El-Nour H
Archives of dermatological research (2013) 3055: 407-13. 214 002 IHC
Probing the functional equivalence of otoferlin and synaptotagmin 1 in exocytosis.
Reisinger E, Bresee C, Neef J, Nair R, Reuter K, Bulankina A, Nouvian R, Koch M, Bückers J, Kastrup L, Roux I, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3113: 4886-95. 214 002 IHC; tested species: mouse
Characterization of Tissue Plasminogen Activator Expression and Trafficking in the Adult Murine Brain.
Stevenson TK, Lawrence DA
eNeuro () 54: . 214 002 IHC; tested species: mouse
Background

Two isoforms of the vitamin D-dependent Ca-binding proteins have been described so far: calbindin D28k, also referred to as CALB 1, D-28k, and CAB 27, and calbindin D29k, also known as calretinin. These proteins are expressed in cells that have to handle a high calcium influx such as brain, bone, teeth, inner ear and others. Calbindins are believed to regulate cellular activity by suppressing or buffering intracellur calcium. In the brain calbindin D28k is a useful marker for specific neuronal cell types. It is particularly concentrated in the dendrites and perikarya of cerebellar Purkinje cells, but is also found in many GABAergic interneurons in the cortex.