Synaptic Systems - Calbindin D28k

Cat. No.: 214 002

Amount: 200 µl

Price: $360.00

Cat. No. 214 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: yes ICC: 1 : 5000 gallery IHC: 1 : 500 gallery IHC-P: 1 : 200 gallery
Immunogen	Full-length recombinant human Calbindin D28k protein (UniProt Id: P05937)
Reactivity	Reacts with: human (P05937), rat (P07171), mouse (P12658), monkey, ape, cow, chicken, zebrafish, turtle, grashopper. Other species not tested yet.
Matching control protein/peptide	214-0P
Data sheet	214_002.pdf

References for Calbindin D28k - 214 002

ICC (3)
IHC (10)

Striosomes control dopamine via dual pathways paralleling canonical basal ganglia circuits.
Lazaridis I, Crittenden JR, Ahn G, Hirokane K, Wickersham IR, Yoshida T, Mahar A, Skara V, Loftus JH, Parvataneni K, Meletis K, et al.
Current biology : CB (2024) : . 214 002 ICC; tested species: mouse

Plasmalogens regulate the AKT-ULK1 signaling pathway to control the position of the axon initial segment.
Ferreira da Silva T, Granadeiro LS, Bessa-Neto D, Luz LL, Safronov BV, Brites P
Progress in neurobiology (2021) 205: 102123. 214 002 ICC; tested species: mouse

Polylaminin recognition by retinal cells.
Hochman-Mendez C, Lacerda de Menezes JR, Sholl-Franco A, Coelho-Sampaio T
Journal of neuroscience research (2014) 921: 24-34. 214 002 ICC; tested species: rat

Retrosplenial inputs drive visual representations in the medial entorhinal cortex.
Dubanet O, Higley MJ
Cell reports (2024) 437: 114470. 214 002 IHC; tested species: mouse

Alpha-synuclein pathology, microgliosis, and parvalbumin neuron loss in the amygdala associated with enhanced fear in the Thy1-aSyn model of Parkinson's disease.
Torres ERS, Stanojlovic M, Zelikowsky M, Bonsberger J, Hean S, Mulligan C, Baldauf L, Fleming S, Masliah E, Chesselet MF, Fanselow MS, et al.
Neurobiology of disease (2021) 158: 105478. 214 002 IHC; tested species: mouse

Loss of Piccolo Function in Rats Induces Cerebellar Network Dysfunction and Pontocerebellar Hypoplasia Type 3-like Phenotypes.
Falck J, Bruns C, Hoffmann-Conaway S, Straub I, Plautz EJ, Orlando M, Munawar H, Rivalan M, Winter Y, Izsvák Z, Schmitz D, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4014: 2943-2959. 214 002 IHC; tested species: rat

Permanent Whisker Removal Reduces the Density of c-Fos+ Cells and the Expression of Calbindin Protein, Disrupts Hippocampal Neurogenesis and Affects Spatial-Memory-Related Tasks.
Gonzalez-Perez O, López-Virgen V, Ibarra-Castaneda N
Frontiers in cellular neuroscience (2018) 12: 132. 214 002 IHC; tested species: mouse

Pharmacological targeting of GSK-3 and NRF2 provides neuroprotection in a preclinical model of tauopathy.
Cuadrado A, Kügler S, Lastres-Becker I
Redox biology (2018) 14: 522-534. 214 002 IHC; tested species: mouse

Abolished perineuronal nets and altered parvalbumin-immunoreactivity in the nucleus reticularis thalami of wildtype and 3xTg mice after experimental stroke.
Härtig W, Appel S, Suttkus A, Grosche J, Michalski D
Neuroscience (2016) 337: 66-87. 214 002 IHC

NGL-2 regulates pathway-specific neurite growth and lamination, synapse formation, and signal transmission in the retina.
Soto F, Watkins KL, Johnson RE, Schottler F, Kerschensteiner D
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3329: 11949-59. 214 002 IHC

Chronic mild stress modulates 5-HT1A and 5-HT2A receptor expression in the cerebellar cortex of NC/Nga atopic-like mice.
Rasul A, Johansson B, Lonne-Rahm SB, Nordlind K, Theodorsson E, El-Nour H
Archives of dermatological research (2013) 3055: 407-13. 214 002 IHC

Probing the functional equivalence of otoferlin and synaptotagmin 1 in exocytosis.
Reisinger E, Bresee C, Neef J, Nair R, Reuter K, Bulankina A, Nouvian R, Koch M, Bückers J, Kastrup L, Roux I, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3113: 4886-95. 214 002 IHC; tested species: mouse

Characterization of Tissue Plasminogen Activator Expression and Trafficking in the Adult Murine Brain.
Stevenson TK, Lawrence DA
eNeuro () 54: . 214 002 IHC; tested species: mouse

Cat. No.: 214 002

Amount: 200 µl

Price: $360.00

References
ICC (3)
IHC (10)

Retrosplenial inputs drive visual representations in the medial entorhinal cortex.
Dubanet O, Higley MJ
Cell reports (2024) 437: 114470. 214 002 IHC; tested species: mouse

Characterization of Tissue Plasminogen Activator Expression and Trafficking in the Adult Murine Brain.
Stevenson TK, Lawrence DA
eNeuro () 54: . 214 002 IHC; tested species: mouse

ICC

IHC

IHC-P

Background

Calbindin D28k (CALB1, D28K or CAB27) and Calretinin (Calbindin D29k) are members of the EF-hand calcium-binding protein family, playing essential roles in intracellular calcium homeostasis, neuroprotection, and signal transduction (1). Calbindin D28k is widely expressed in tissues that experience high calcium influx, such as the brain, sensory organs, endocrine tissues, and the enteric nervous system (ENS). In the central nervous system (CNS), Calbindin D28k is an important marker for specific neuronal populations, particularly in Purkinje cells of the cerebellum and GABAergic and glutamatergic neurons of the cortex (2). Outside the CNS, Calbindin D28k plays crucial roles in non-neuronal tissues. In the retina, it contributes to photoreceptor function and calcium signaling (3). In the pancreas, it is present in α- and β-cells (4). Additionally, it was reported that 31% of myenteric neurons and up to 95% of submucosal neurons in the human ENS express calbindin, suggesting its involvement in both motor and secretory functions (5).

1 ) Schwaller B et al., Cerebellum (2002)
2 ) Flace P et al., Anat Rec (Hoboken) (2014)
3 ) Gu YN et al., Exp Eye Res (2016)
4 ) Janssen SW et al., J Histochem Cytochem (2002)
5 ) Zetzmann K et al., Int J Mol Sci (2018)

Protocols

AP Detection
ECL Detection
Fluorescent detection
Pre-adsorption protocol

Standard protocol

ICC

PFA fixation
Pre-adsorption protocol

IHC

Free floating
Slide mounted
Tissue preparation
Pre-adsorption protocol

IHC-P

Chromogenic detection
Fluorescent detection
Tissue preparation
Pre-adsorption protocol

OTHER

Preabsorbtion

Other products for this target

Calbindin D28k antibody - 214 002

References for Calbindin D28k - 214 002