|Cat. No. 214 011BT||100 µg purified IgG, lyophilized, biotin-labeled. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 (AP staining)
ICC: not tested yet
IHC: 1 : 500 gallery
IHC-P/FFPE: not tested yet
|Subtype||IgG1 (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 3 to 251 from human CalbindinD28k (UniProt Id: P05937)|
Epitop: AA 3 to 251 from human CalbindinD28k (UniProt Id: P05937)
Reacts with: human (P05937), rat (P07171), mouse (P12658), zebrafish.
Other species not tested yet.
|Specificity||Specific for calbindin D28k.|
|Matching control protein/peptide||214-0P|
Two isoforms of the vitamin D-dependent Ca-binding proteins have been described so far: calbindin D28k, also referred to as CALB 1, D-28k, and CAB 27, and calbindin D29k, also known as calretinin. These proteins are expressed in cells that have to handle a high calcium influx such as brain, bone, teeth, inner ear and others. Calbindins are believed to regulate cellular activity by suppressing or buffering intracellur calcium. In the brain calbindin D28k is a useful marker for specific neuronal cell types. It is particularly concentrated in the dendrites and perikarya of cerebellar Purkinje cells, but is also found in many GABAergic interneurons in the cortex.