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Calbindin D28k - 214 011BT

A vitamin D-dependent calcium binding protein
Monoclonal mouse purified IgG
Cat. No.: 214 011BT
Amount: 100 µg
Price: $465.00
Cat. No. 214 011BT 100 µg purified IgG, lyophilized, biotin-labeled. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 (AP staining)
IP: yes
ICC: not tested yet
IHC: 1 : 500 gallery  
IHC-P/FFPE: not tested yet
Label biotin
Clone 351C10
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 3 to 251 from human CalbindinD28k (UniProt Id: P05937)
Epitop Epitop: AA 3 to 251 from human CalbindinD28k (UniProt Id: P05937)
Reactivity Reacts with: human (P05937), rat (P07171), mouse (P12658), zebrafish.
Other species not tested yet.
Specificity Specific for calbindin D28k.
Matching control protein/peptide 214-0P
Data sheet 214_011bt.pdf
Cat. No.: 214 011BT
Quantity: 100 µg
Price: $465.00
Background

Two isoforms of the vitamin D-dependent Ca-binding proteins have been described so far: calbindin D28k, also referred to as CALB 1, D-28k, and CAB 27, and calbindin D29k, also known as calretinin. These proteins are expressed in cells that have to handle a high calcium influx such as brain, bone, teeth, inner ear and others. Calbindins are believed to regulate cellular activity by suppressing or buffering intracellur calcium. In the brain calbindin D28k is a useful marker for specific neuronal cell types. It is particularly concentrated in the dendrites and perikarya of cerebellar Purkinje cells, but is also found in many GABAergic interneurons in the cortex.