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Calbindin D28k - 214 011C3

A vitamin D-dependent calcium binding protein
Monoclonal mouse purified IgG
Cat. No.: 214 011C3
Amount: 100 µg
Price: 465.00 €
Cat. No. 214 011C3 100 µg purified IgG, lyophilized, fluorescence-labeled with Oyster® 550

Fluorescence labeled antibodies conjugated to Oyster dyes from Luminartis GmbH (formerly Denovo Biolabels) allow the usage of established CyDye fluorescence detection systems. Oyster-488 has similar exitation and emission properties as Cy2, Oyster-550 as Cy3, and Oyster-650 is comparable to Cy5.
For our custom antibody labeling service Oyster-500 is also available.
Oyster dyes do not form dimers and therefore exhibit less internal quenching. This allows a higher labeling degree and consequently brighter conjugates.

Oyster dyes, Oyster-488, Oyster-500, Oyster-550, and Oyster-650 are trademarks of Luminartis GmbH, Muenster/Germany.

CyDyes, Cy2, Cy3, and Cy5 are trademarks of GE-Healthcare Ltd.

. Albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.The mounting agent Aquatex®(Merck Chemicals) is not compatible with Oyster dyes!
Applications WB: N/A
IP: N/A
ICC: not tested yet
IHC: 1 : 200 gallery  
IHC-P/FFPE: not tested yet
Label Oyster 550
Clone 351C10
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 3 to 251 from human CalbindinD28k (UniProt Id: P05937)
Epitop Epitop: AA 3 to 251 from human CalbindinD28k (UniProt Id: P05937)
Reactivity Reacts with: human (P05937), rat (P07171), mouse (P12658), zebrafish.
Other species not tested yet.
Specificity Specific for calbindin D28k.
Matching control protein/peptide 214-0P
Data sheet 214_011c3.pdf
Cat. No.: 214 011C3
Quantity: 100 µg
Price: 465.00 €
Background

Two isoforms of the vitamin D-dependent Ca-binding proteins have been described so far: calbindin D28k, also referred to as CALB 1, D-28k, and CAB 27, and calbindin D29k, also known as calretinin. These proteins are expressed in cells that have to handle a high calcium influx such as brain, bone, teeth, inner ear and others. Calbindins are believed to regulate cellular activity by suppressing or buffering intracellur calcium