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Calbindin D28k - 214 011C3

A vitamin D-dependent calcium binding protein
Monoclonal mouse purified IgG
Cat. No.: 214 011C3
Amount: 100 µg
Price: $465.00
Cat. No. 214 011C3 100 µg purified IgG, lyophilized, fluorescence-labeled with Oyster® 550

Fluorescence labeled antibodies conjugated to Oyster dyes from Luminartis GmbH (formerly Denovo Biolabels) allow the usage of established CyDye fluorescence detection systems. Oyster-488 has similar exitation and emission properties as Cy2, Oyster-550 as Cy3, and Oyster-650 is comparable to Cy5.
For our custom antibody labeling service Oyster-500 is also available.
Oyster dyes do not form dimers and therefore exhibit less internal quenching. This allows a higher labeling degree and consequently brighter conjugates.

Oyster dyes, Oyster-488, Oyster-500, Oyster-550, and Oyster-650 are trademarks of Luminartis GmbH, Muenster/Germany.

CyDyes, Cy2, Cy3, and Cy5 are trademarks of GE-Healthcare Ltd.

. Albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.The mounting agent Aquatex®(Merck Chemicals) is not compatible with Oyster dyes!
Applications WB: N/A
IP: N/A
ICC: not tested yet
IHC: 1 : 200 gallery  
IHC-P/FFPE: not tested yet
Label Oyster 550
Clone 351C10
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 3 to 251 from human CalbindinD28k (UniProt Id: P05937)
Epitop Epitop: AA 3 to 251 from human CalbindinD28k (UniProt Id: P05937)
Reactivity Reacts with: human (P05937), rat (P07171), mouse (P12658), zebrafish.
Other species not tested yet.
Specificity Specific for calbindin D28k.
Matching control protein/peptide 214-0P
Data sheet 214_011c3.pdf

References for Calbindin D28k - 214 011C3

Stem cell regionalization during olfactory bulb neurogenesis depends on regulatory interactions between Vax1 and Pax6.
Coré N, Erni A, Hoffmann HM, Mellon PL, Saurin AJ, Beclin C, Cremer H
eLife (2020) 9: . 214 011C3 IHC; tested species: mouse
Cat. No.: 214 011C3
Quantity: 100 µg
Price: $465.00
Stem cell regionalization during olfactory bulb neurogenesis depends on regulatory interactions between Vax1 and Pax6.
Coré N, Erni A, Hoffmann HM, Mellon PL, Saurin AJ, Beclin C, Cremer H
eLife (2020) 9: . 214 011C3 IHC; tested species: mouse
Background

Two isoforms of the vitamin D-dependent Ca-binding proteins have been described so far: calbindin D28k, also referred to as CALB 1, D-28k, and CAB 27, and calbindin D29k, also known as calretinin. These proteins are expressed in cells that have to handle a high calcium influx such as brain, bone, teeth, inner ear and others. Calbindins are believed to regulate cellular activity by suppressing or buffering intracellur calcium. In the brain calbindin D28k is a useful marker for specific neuronal cell types. It is particularly concentrated in the dendrites and perikarya of cerebellar Purkinje cells, but is also found in many GABAergic interneurons in the cortex.