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VGAT cytoplasmic domain - 131-0P

GABA transporter in the membrane of synaptic vesicles
control peptide
Cat. No.: 131-0P
Amount: 100 µg
Price: 90.00 €
Cat. No. 131-0P 100 µg peptide, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Control peptides should be stored at -20°C when still lyophilized!
Immunogen Synthetic peptide corresponding to AA 75 to 87 from rat VGAT (UniProt Id: O35458)
Recommended dilution Optimal concentrations should be determined by the end-user.
Matching antibodies 131 002, 131 003, 131 011, 131 011BT, 131 011C2, 131 011C3, 131 008, 131 308
Remarks This control peptide consists of the synthetic peptide (AEPPVEGDIHYQR) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet 131-0p.pdf
Cat. No.: 131-0P
Quantity: 100 µg
Price: 90.00 €
Background
The vesicular GABA transporter VGAT is responsible for uptake and storage of GABA and glycine by synaptic vesicles in the central nervous system. For this reason it is frequently referred to as the vesicular inhibitory aminoacid transporter VIAAT. It is different from the plasma membrane transporters in that it is driven by a proton electrochemical gradient across the vesicle membrane. So far, only one isoform is known. VGAT is currently the best marker for inhibitory nerve terminals.
Protocols