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VGAT antibody cytoplasmic domain - 131 011C3

VGAT or VIAAT is the GABA and glycine transporter in the membrane of synaptic vesicles
Monoclonal mouse purified IgG
Cat. No.: 131 011C3
Amount: 100 µg
Price: $465.00
Cat. No. 131 011C3 100 µg purified IgG, lyophilized, fluorescence-labeled with Sulfo-Cyanine 3. Albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications WB: N/A
IP: N/A
ICC: 1 : 1000 up to 1 : 2000 gallery  
IHC: 1 : 500 up to 1 : 1000
IHC-P/FFPE: not tested yet
Label Sulfo-Cyanine 3
Clone 117G4
Subtype IgG3 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 75 to 87 from rat VGAT (UniProt Id: O35458)
Epitop Epitop: AA 75 to 87 from rat VGAT (UniProt Id: O35458)
Reactivity Reacts with: human (Q9H598), rat (O35458), mouse (O35633), Guinea pig, monkey.
Other species not tested yet.
Specificity Specific for mammalian VGAT. K.O.
Matching control protein/peptide 131-0P
Data sheet 131_011c3.pdf

References for VGAT - 131 011C3

Cellular distribution of the NMDA-receptor activated synapto-nuclear messenger Jacob in the rat brain.
Mikhaylova M, Karpova A, Bär J, Bethge P, YuanXiang P, Chen Y, Zuschratter W, Behnisch T, Kreutz MR
Brain structure & function (2014) 2193: 843-60. 131 011C3 ICC, IHC
Cellular distribution of the NMDA-receptor activated synapto-nuclear messenger Jacob in the rat brain.
Mikhaylova M, Karpova A, Bär J, Bethge P, YuanXiang P, Chen Y, Zuschratter W, Behnisch T, Kreutz MR
Brain structure & function (2014) 2193: 843-60. 131 011C3 ICC, IHC
Cat. No.: 131 011C3
Quantity: 100 µg
Price: $465.00
Cellular distribution of the NMDA-receptor activated synapto-nuclear messenger Jacob in the rat brain.
Mikhaylova M, Karpova A, Bär J, Bethge P, YuanXiang P, Chen Y, Zuschratter W, Behnisch T, Kreutz MR
Brain structure & function (2014) 2193: 843-60. 131 011C3 ICC, IHC
Cellular distribution of the NMDA-receptor activated synapto-nuclear messenger Jacob in the rat brain.
Mikhaylova M, Karpova A, Bär J, Bethge P, YuanXiang P, Chen Y, Zuschratter W, Behnisch T, Kreutz MR
Brain structure & function (2014) 2193: 843-60. 131 011C3 ICC, IHC
Background
The vesicular GABA transporter VGAT is responsible for uptake and storage of GABA and glycine by synaptic vesicles in the central nervous system. For this reason it is frequently referred to as the vesicular inhibitory aminoacid transporter VIAAT. It is different from the plasma membrane transporters in that it is driven by a proton electrochemical gradient across the vesicle membrane. So far, only one isoform is known. VGAT is currently the best marker for inhibitory nerve terminals.