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Home > Products > 131 011C3

VGAT antibody cytoplasmic domain - 131 011C3 K.O.

VGAT or VIAAT is the GABA and glycine transporter in the membrane of synaptic vesicles
Mouse monoclonal purified IgG
Cat. No.: 131 011C3
Amount: 100 µg
Price: $475.00
Cat. No. 131 011C3 100 µg purified IgG, lyophilized, fluorescence-labeled with Cyanine 3.

Fluorescence labeled antibodies conjugated to (Sulfo-)Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy.

Cyanine 2: λex 492 nm / λem 508 nm
Sulfo-Cyanine 3: λex 554 nm / λem 568 nm
Sulfo-Cyanine 5: λex 649 nm / λem 667 nm

(Sulfo-)Cyanine 5 dyes are well suited for STORM high resolution microscopy.
Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates.

 Albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 1000 up to 1 : 2000 gallery  
IHC: 1 : 500 up to 1 : 1000
IHC-P: not tested yet
Label Sulfo-Cyanine 3
Clone 117G4
Subtype IgG3 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the amino terminus of rat VGAT (UniProt Id: O35458)
Reactivity Reacts with: human (Q9H598), rat (O35458), mouse (O35633), Guinea pig, monkey.
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 131-0P
Data sheet 131_011c3.pdf

References for VGAT - 131 011C3

Cellular distribution of the NMDA-receptor activated synapto-nuclear messenger Jacob in the rat brain.
Mikhaylova M, Karpova A, Bär J, Bethge P, YuanXiang P, Chen Y, Zuschratter W, Behnisch T, Kreutz MR
Brain structure & function (2014) 2193: 843-60. 131 011C3 ICC, IHC
Cellular distribution of the NMDA-receptor activated synapto-nuclear messenger Jacob in the rat brain.
Mikhaylova M, Karpova A, Bär J, Bethge P, YuanXiang P, Chen Y, Zuschratter W, Behnisch T, Kreutz MR
Brain structure & function (2014) 2193: 843-60. 131 011C3 ICC, IHC
Cat. No.: 131 011C3
Amount: 100 µg
Price: $475.00
Cellular distribution of the NMDA-receptor activated synapto-nuclear messenger Jacob in the rat brain.
Mikhaylova M, Karpova A, Bär J, Bethge P, YuanXiang P, Chen Y, Zuschratter W, Behnisch T, Kreutz MR
Brain structure & function (2014) 2193: 843-60. 131 011C3 ICC, IHC
Cellular distribution of the NMDA-receptor activated synapto-nuclear messenger Jacob in the rat brain.
Mikhaylova M, Karpova A, Bär J, Bethge P, YuanXiang P, Chen Y, Zuschratter W, Behnisch T, Kreutz MR
Brain structure & function (2014) 2193: 843-60. 131 011C3 ICC, IHC
ICC
Background
The vesicular GABA transporter VGAT is responsible for uptake and storage of GABA and glycine by synaptic vesicles in the central nervous system. For this reason it is frequently referred to as the vesicular inhibitory aminoacid transporter VIAAT. It is different from the plasma membrane transporters in that it is driven by a proton electrochemical gradient across the vesicle membrane. So far, only one isoform is known. VGAT is currently the best marker for inhibitory nerve terminals.
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