Tailor-made Antibodies
and Tools for Life Science
quick order | cart
?
|
login
Home|||||Technical Support
Home > Products > 131 011BT

VGAT antibody cytoplasmic domain - 131 011BT K.O.

VGAT or VIAAT is the GABA and glycine transporter in the membrane of synaptic vesicles
Mouse monoclonal purified IgG
Cat. No.: 131 011BT
Amount: 100 µg
Price: $475.00
Cat. No. 131 011BT 100 µg purified IgG, lyophilized, labeled with Biotin.

Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality.
Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems.
The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known.

 For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 500 up to 1 : 2000 (AP staining) (see remarks)
IP: yes
ICC: 1 : 200 up to 1 : 1000 gallery  
IHC: 1 : 100 up to 1 : 1000 gallery  
IHC-P: not tested yet
Label biotin
Clone 117G4
Subtype IgG3 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the amino terminus of rat VGAT (UniProt Id: O35458)
Reactivity Reacts with: human (Q9H598), rat (O35458), mouse (O35633), Guinea pig, monkey.
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 131-0P
Remarks

WB:  VGAT aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet 131_011bt.pdf
Cat. No.: 131 011BT
Amount: 100 µg
Price: $475.00
ICC
IHC
Background
The vesicular GABA transporter VGAT is responsible for uptake and storage of GABA and glycine by synaptic vesicles in the central nervous system. For this reason it is frequently referred to as the vesicular inhibitory aminoacid transporter VIAAT. It is different from the plasma membrane transporters in that it is driven by a proton electrochemical gradient across the vesicle membrane. So far, only one isoform is known. VGAT is currently the best marker for inhibitory nerve terminals.
Other products for this target