VGAT antibody cytoplasmic domain - 131 011BT K.O.

VGAT or VIAAT is the GABA and glycine transporter in the membrane of synaptic vesicles

Mouse monoclonal purified IgG

Cat. No.: 131 011BT

Amount: 100 µg

Price: $475.00

Cat. No. 131 011BT	100 µg purified IgG, lyophilized, labeled with Biotin. Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems. The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 up to 1 : 2000 (AP staining) (see remarks) IP: yes ICC: 1 : 200 up to 1 : 1000 gallery IHC: 1 : 100 up to 1 : 1000 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	biotin
Clone	117G4
Subtype	IgG3 (κ light chain)
Immunogen	Synthetic peptide corresponding to residues near the amino terminus of rat VGAT (UniProt Id: O35458)
Reactivity	Reacts with: human (Q9H598), rat (O35458), mouse (O35633), Guinea pig, monkey. Other species not tested yet.
Specificity	K.O. validated
Matching control protein/peptide	131-0P
Remarks	WB: VGAT aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet	131_011bt.pdf

Cat. No.: 131 011BT

Amount: 100 µg

Price: $475.00

ICC

IHC

Background

The vesicular GABA transporter VGAT is responsible for uptake and storage of GABA and glycine by synaptic vesicles in the central nervous system. For this reason it is frequently referred to as the vesicular inhibitory aminoacid transporter VIAAT. It is different from the plasma membrane transporters in that it is driven by a proton electrochemical gradient across the vesicle membrane. So far, only one isoform is known. VGAT is currently the best marker for inhibitory nerve terminals.

1 ) Chaudhry FA et al., J. Neurosci. (1998)
2 ) McIntire SL et al., Nature (1997)
3 ) Sagné C et al., FEBS Lett. (1997)
4 ) Hell JW et al., EMBO J. (1988)

Protocols

Standard protocol

ICC

IHC

OTHER

Preabsorbtion

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