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Home > Products > 131 008

VGAT antibody cytoplasmic domain - 131 008 K.O.

VGAT or VIAAT is the GABA and glycine transporter in the membrane of synaptic vesicles
Rabbit monoclonal recombinant IgG
Cat. No.: 131 008
Amount: 50 µg
Price: $415.00
Cat. No. 131 008 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 2000 AP staining (see remarks) gallery  
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 500 up to 1 : 1000 (see remarks) gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone Rb117G4
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the amino terminus of rat VGAT (UniProt Id: O35458)
Reactivity Reacts with: human (Q9H598), rat (O35458), mouse (O35633).
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 131-0P
Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 117G4. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
WB: VGAT aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
IHC-P: Antigen retrieval at pH 9.0 (10 mM Tris pH 9.0, 1mM EDTA, 0.05 % Tween20) is recommended.
Data sheet 131_008.pdf

References for VGAT - 131 008

Low-frequency RTMS attenuates social impairment in the VPA-induced mouse model.
Wang X, Li Y, Li R, Yuan L, Hua Y, Cai Y, Liu X
Behavioural brain research (2024) 472: 115156. 131 008 WB, IHC; tested species: mouse
Activity-dependent tau cleavage by caspase-3 promotes neuronal dysfunction and synaptotoxicity.
Opland CK, Bryan MR, Harris B, McGillion-Moore J, Tian X, Chen Y, Itano MS, Diering GH, Meeker RB, Cohen TJ
iScience (2023) 266: 106905. 131 008 ICC; tested species: mouse
Super-resolution imaging of synaptic scaffold proteins in rat hippocampal neurons.
Guzikowski NJ, Kavalali ET
STAR protocols (2023) 41: 102080. 131 008 ICC; tested species: rat
Nano-organization of spontaneous GABAergic transmission directs its autonomous function in neuronal signaling.
Guzikowski NJ, Kavalali ET
Cell reports (2022) 406: 111172. 131 008 ICC; tested species: rat
Long-Term Cultures of Spinal Cord Interneurons.
Vargova I, Kriska J, Kwok JCF, Fawcett JW, Jendelova P
Frontiers in cellular neuroscience (2022) 16: 827628. 131 008 ICC; tested species: mouse
Role of Aberrant Spontaneous Neurotransmission in SNAP25-Associated Encephalopathies.
Alten B, Zhou Q, Shin OH, Esquivies L, Lin PY, White KI, Sun R, Chung WK, Monteggia LM, Brunger AT, Kavalali ET, et al.
Neuron (2020) : . 131 008 ICC; tested species: mouse
Low-frequency RTMS attenuates social impairment in the VPA-induced mouse model.
Wang X, Li Y, Li R, Yuan L, Hua Y, Cai Y, Liu X
Behavioural brain research (2024) 472: 115156. 131 008 WB, IHC; tested species: mouse
Synaptogenic gene therapy with FGF22 improves circuit plasticity and functional recovery following spinal cord injury.
Aljović A, Jacobi A, Marcantoni M, Kagerer F, Loy K, Kendirli A, Bräutigam J, Fabbio L, Van Steenbergen V, Pleśniar K, Kerschensteiner M, et al.
EMBO molecular medicine (2023) : e16111. 131 008 IHC; tested species: mouse
Exploratory study of the long-term footprint of deep brain stimulation on brain metabolism and neuroplasticity in an animal model of obesity.
Casquero-Veiga M, Bueno-Fernandez C, Romero-Miguel D, Lamanna-Rama N, Nacher J, Desco M, Soto-Montenegro ML
Scientific reports (2021) 111: 5580. 131 008 IHC; tested species: rat
TDP-43 proteinopathy occurs independently of autophagic substrate accumulation and underlies nuclear defects in Niemann-Pick C disease.
Liu EA, Mori E, Hamasaki F, Lieberman AP
Neuropathology and applied neurobiology (2021) 477: 1019-1032. 131 008 IHC; tested species: mouse
Contribution of the astrocytic tau pathology to synapse loss in progressive supranuclear palsy and corticobasal degeneration.
Briel N, Pratsch K, Roeber S, Arzberger T, Herms J
Brain pathology (Zurich, Switzerland) (2021) 314: e12914. 131 008 IHC-P; tested species: human
Cat. No.: 131 008
Amount: 50 µg
Price: $415.00
Low-frequency RTMS attenuates social impairment in the VPA-induced mouse model.
Wang X, Li Y, Li R, Yuan L, Hua Y, Cai Y, Liu X
Behavioural brain research (2024) 472: 115156. 131 008 WB, IHC; tested species: mouse
Activity-dependent tau cleavage by caspase-3 promotes neuronal dysfunction and synaptotoxicity.
Opland CK, Bryan MR, Harris B, McGillion-Moore J, Tian X, Chen Y, Itano MS, Diering GH, Meeker RB, Cohen TJ
iScience (2023) 266: 106905. 131 008 ICC; tested species: mouse
Super-resolution imaging of synaptic scaffold proteins in rat hippocampal neurons.
Guzikowski NJ, Kavalali ET
STAR protocols (2023) 41: 102080. 131 008 ICC; tested species: rat
Nano-organization of spontaneous GABAergic transmission directs its autonomous function in neuronal signaling.
Guzikowski NJ, Kavalali ET
Cell reports (2022) 406: 111172. 131 008 ICC; tested species: rat
Long-Term Cultures of Spinal Cord Interneurons.
Vargova I, Kriska J, Kwok JCF, Fawcett JW, Jendelova P
Frontiers in cellular neuroscience (2022) 16: 827628. 131 008 ICC; tested species: mouse
Role of Aberrant Spontaneous Neurotransmission in SNAP25-Associated Encephalopathies.
Alten B, Zhou Q, Shin OH, Esquivies L, Lin PY, White KI, Sun R, Chung WK, Monteggia LM, Brunger AT, Kavalali ET, et al.
Neuron (2020) : . 131 008 ICC; tested species: mouse
Low-frequency RTMS attenuates social impairment in the VPA-induced mouse model.
Wang X, Li Y, Li R, Yuan L, Hua Y, Cai Y, Liu X
Behavioural brain research (2024) 472: 115156. 131 008 WB, IHC; tested species: mouse
Synaptogenic gene therapy with FGF22 improves circuit plasticity and functional recovery following spinal cord injury.
Aljović A, Jacobi A, Marcantoni M, Kagerer F, Loy K, Kendirli A, Bräutigam J, Fabbio L, Van Steenbergen V, Pleśniar K, Kerschensteiner M, et al.
EMBO molecular medicine (2023) : e16111. 131 008 IHC; tested species: mouse
Exploratory study of the long-term footprint of deep brain stimulation on brain metabolism and neuroplasticity in an animal model of obesity.
Casquero-Veiga M, Bueno-Fernandez C, Romero-Miguel D, Lamanna-Rama N, Nacher J, Desco M, Soto-Montenegro ML
Scientific reports (2021) 111: 5580. 131 008 IHC; tested species: rat
TDP-43 proteinopathy occurs independently of autophagic substrate accumulation and underlies nuclear defects in Niemann-Pick C disease.
Liu EA, Mori E, Hamasaki F, Lieberman AP
Neuropathology and applied neurobiology (2021) 477: 1019-1032. 131 008 IHC; tested species: mouse
Contribution of the astrocytic tau pathology to synapse loss in progressive supranuclear palsy and corticobasal degeneration.
Briel N, Pratsch K, Roeber S, Arzberger T, Herms J
Brain pathology (Zurich, Switzerland) (2021) 314: e12914. 131 008 IHC-P; tested species: human
WB
ICC
ICC
IHC
IHC
IHC
IHC-P
Background
The vesicular GABA transporter VGAT is responsible for uptake and storage of GABA and glycine by synaptic vesicles in the central nervous system. For this reason it is frequently referred to as the vesicular inhibitory aminoacid transporter VIAAT. It is different from the plasma membrane transporters in that it is driven by a proton electrochemical gradient across the vesicle membrane. So far, only one isoform is known. VGAT is currently the best marker for inhibitory nerve terminals.
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