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VGAT antibody cytoplasmic domain - 131 008

VGAT or VIAAT is the GABA and glycine transporter in the membrane of synaptic vesicles
Monoclonal Recombinant rabbit purified IgG
Cat. No.: 131 008
Amount: 50 µg
Price: $415.00
Cat. No. 131 008 50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 up to 1 : 2000 AP staining (see remarks) gallery  
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P/FFPE: 1 : 500 up to 1 : 1000 (see remarks) gallery  
Clone Rb117G4
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 75 to 87 from rat VGAT (UniProt Id: O35458)
Epitop Epitop: AA 75 to 87 from rat VGAT (UniProt Id: O35458)
Reactivity Reacts with: human (Q9H598), rat (O35458), mouse (O35633).
Other species not tested yet.
Specificity Specific for VGAT K.O.
Matching control protein/peptide 131-0P
Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 117G4. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
WB: VGAT aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
IHC-P: Antigen retrieval at pH 9.0 (10 mM Tris pH 9.0, 1mM EDTA, 0.05 % Tween20) is recommended.

Data sheet 131_008.pdf

References for VGAT - 131 008

Role of Aberrant Spontaneous Neurotransmission in SNAP25-Associated Encephalopathies.
Alten B, Zhou Q, Shin OH, Esquivies L, Lin PY, White KI, Sun R, Chung WK, Monteggia LM, Brunger AT, Kavalali ET, et al.
Neuron (2020) : . 131 008 ICC; tested species: mouse
Exploratory study of the long-term footprint of deep brain stimulation on brain metabolism and neuroplasticity in an animal model of obesity.
Casquero-Veiga M, Bueno-Fernandez C, Romero-Miguel D, Lamanna-Rama N, Nacher J, Desco M, Soto-Montenegro ML
Scientific reports (2021) 111: 5580. 131 008 IHC; tested species: rat
Cat. No.: 131 008
Quantity: 50 µg
Price: $415.00
Role of Aberrant Spontaneous Neurotransmission in SNAP25-Associated Encephalopathies.
Alten B, Zhou Q, Shin OH, Esquivies L, Lin PY, White KI, Sun R, Chung WK, Monteggia LM, Brunger AT, Kavalali ET, et al.
Neuron (2020) : . 131 008 ICC; tested species: mouse
Exploratory study of the long-term footprint of deep brain stimulation on brain metabolism and neuroplasticity in an animal model of obesity.
Casquero-Veiga M, Bueno-Fernandez C, Romero-Miguel D, Lamanna-Rama N, Nacher J, Desco M, Soto-Montenegro ML
Scientific reports (2021) 111: 5580. 131 008 IHC; tested species: rat
Background
The vesicular GABA transporter VGAT is responsible for uptake and storage of GABA and glycine by synaptic vesicles in the central nervous system. For this reason it is frequently referred to as the vesicular inhibitory aminoacid transporter VIAAT. It is different from the plasma membrane transporters in that it is driven by a proton electrochemical gradient across the vesicle membrane. So far, only one isoform is known. VGAT is currently the best marker for inhibitory nerve terminals.