Synaptic Systems

Cat. No.: 131 008

Amount: 50 µg

Price: $420.00

Cat. No. 131 008	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 2000 AP staining (see remarks) gallery ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: 1 : 500 up to 1 : 1000 (see remarks) gallery
Clone	Rb117G4
Subtype	IgG1 (κ light chain)
Immunogen	Synthetic peptide corresponding to residues near the amino terminus of rat VGAT (UniProt Id: O35458)
Reactivity	Reacts with: human (Q9H598), rat (O35458), mouse (O35633). Other species not tested yet.
Specificity	K.O. validated
Matching control protein/peptide	131-0P
Remarks	This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 117G4. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells. WB: VGAT aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. IHC-P: Antigen retrieval at pH 9.0 (10 mM Tris pH 9.0, 1mM EDTA, 0.05 % Tween20) is recommended.
Data sheet	131_008.pdf

Low-frequency RTMS attenuates social impairment in the VPA-induced mouse model.
Wang X, Li Y, Li R, Yuan L, Hua Y, Cai Y, Liu X
Behavioural brain research (2024) 472: 115156. 131 008 WB, IHC; tested species: mouse

Cat. No.: 131 008

Amount: 50 µg

Price: $420.00

ICC

IHC

IHC-P

Background

Protocols

VGAT antibody cytoplasmic domain - 131 008 K.O.