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VGAT antibody cytoplasmic domain - 131 308

VGAT or VIAAT is the GABA and glycine transporter in the membrane of synaptic vesicles
Monoclonal Recombinant Guinea pig recombinant IgG
Cat. No.: 131 308
Amount: 50 µg
Price: $415.00
Cat. No. 131 308 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 (AP staining) (see remarks) gallery  
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P/FFPE: 1 : 400 gallery  
Clone Gp117G4
Subtype IgG2 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 75 to 87 from rat VGAT (UniProt Id: O35458)
Epitop Epitop: AA 75 to 87 from rat VGAT (UniProt Id: O35458)
Reactivity Reacts with: human (Q9H598), rat (O35458), mouse (O35633), Guinea pig, ape.
Other species not tested yet.
Specificity Specific for mammalian VGAT. K.O.
Matching control protein/peptide 131-0P
Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody 117G4. The constant regions of the heavy and light chains have been replaced by Guinea pig specific sequences. Therefore, the antibody can be used with standard anti-Guinea pig secondary reagents. The antibody has been expressed in mammalian cells.
WB: VGAT aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

Data sheet 131_308.pdf
Cat. No.: 131 308
Quantity: 50 µg
Price: $415.00
Background
The vesicular GABA transporter VGAT is responsible for uptake and storage of GABA and glycine by synaptic vesicles in the central nervous system. For this reason it is frequently referred to as the vesicular inhibitory aminoacid transporter VIAAT. It is different from the plasma membrane transporters in that it is driven by a proton electrochemical gradient across the vesicle membrane. So far, only one isoform is known. VGAT is currently the best marker for inhibitory nerve terminals.