Synaptic Systems

Cat. No.: 131 308

Amount: 50 µg

Price: $425.00

Cat. No. 131 308	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 400 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Gp117G4
Subtype	IgG2 (κ light chain)
Immunogen	Synthetic peptide corresponding to residues near the amino terminus of rat VGAT (UniProt Id: O35458)
Reactivity	Reacts with: human (Q9H598), rat (O35458), mouse (O35633), Guinea pig, ape. Other species not tested yet.
Specificity	K.O. validated
Matching control protein/peptide	131-0P
Remarks	This antibody is a chimeric antibody based on the well known monoclonal mouse antibody 117G4. The constant regions of the heavy and light chains have been replaced by Guinea pig specific sequences. Therefore, the antibody can be used with standard anti-Guinea pig secondary reagents. The antibody has been expressed in mammalian cells. WB: VGAT aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet	131_308.pdf

References for VGAT - 131 308

MAD2B promotes podocyte injury through regulating Numb-dependent Notch 1 pathway in diabetic nephropathy.
Li MR, Lei CT, Tang H, Yin XJ, Hao Z, Qiu Y, Xie YR, Zeng JY, Su H, Zhang C
International journal of biological sciences (2022) 185: 1896-1911. 131 308 ICC; tested species: rat

The synaptic scaffold protein MPP2 interacts with GABAA receptors at the periphery of the postsynaptic density of glutamatergic synapses.
Schmerl B, Gimber N, Kuropka B, Stumpf A, Rentsch J, Kunde SA, von Sivers J, Ewers H, Schmitz D, Freund C, Schmoranzer J, et al.
PLoS biology (2022) 203: e3001503. 131 308 ICC; tested species: rat

The Alzheimer susceptibility gene BIN1 induces isoform-dependent neurotoxicity through early endosome defects.
Lambert E, Saha O, Soares Landeira B, Melo de Farias AR, Hermant X, Carrier A, Pelletier A, Gadaut J, Davoine L, Dupont C, Amouyel P, et al.
Acta neuropathologica communications (2022) 101: 4. 131 308 ICC, IHC; tested species: rat

A DARPin-based molecular toolset to probe gephyrin and inhibitory synapse biology.
Campbell BFN, Dittmann A, Dreier B, Plückthun A, Tyagarajan SK
eLife (2022) 11: . 131 308 ICC, IHC; tested species: rat

Spinal Cord Neuronal Network Formation in a 3D Printed Reinforced Matrix-A Model System to Study Disease Mechanisms.
Fischhaber N, Faber J, Bakirci E, Dalton PD, Budday S, Villmann C, Schaefer N
Advanced healthcare materials (2021) 1019: e2100830. 131 308 ICC; tested species: mouse

Repetitive concussions promote microglia-mediated engulfment of presynaptic excitatory input associated with cognitive dysfunction.
Chahin M, Mutschler J, Dzhuleva SP, Dieterle C, Jimenez LR, Bhattarai SR, Van Steenbergen V, Bareyre FM
Communications biology (2025) 81: 335. 131 308 IHC; tested species: mouse

Anatomy of superior olivary complex and lateral lemniscus in Etruscan shrew.
Zacher AC, Felmy F
Scientific reports (2024) 141: 14734. 131 308 IHC

Facial neuromuscular junctions and brainstem nuclei are the target of tetanus neurotoxin in cephalic tetanus.
Fabris F, Varani S, Tonellato M, Matak I, Šoštarić P, Meglić P, Caleo M, Megighian A, Rossetto O, Montecucco C, Pirazzini M, et al.
JCI insight (2023) 811: . 131 308 IHC; tested species: mouse,rat

Cat. No.: 131 308

Amount: 50 µg

Price: $425.00

Anatomy of superior olivary complex and lateral lemniscus in Etruscan shrew.
Zacher AC, Felmy F
Scientific reports (2024) 141: 14734. 131 308 IHC

ICC

IHC

IHC-P

Background

The vesicular GABA transporter VGAT is responsible for uptake and storage of GABA and glycine by synaptic vesicles in the central nervous system. For this reason it is frequently referred to as the vesicular inhibitory aminoacid transporter VIAAT. It is different from the plasma membrane transporters in that it is driven by a proton electrochemical gradient across the vesicle membrane. So far, only one isoform is known. VGAT is currently the best marker for inhibitory nerve terminals.

1 ) Chaudhry FA et al., J. Neurosci. (1998)
2 ) McIntire SL et al., Nature (1997)
3 ) Sagné C et al., FEBS Lett. (1997)
4 ) Hell JW et al., EMBO J. (1988)

Protocols

ICC

IHC

IHC-P

OTHER

Preabsorbtion

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VGAT antibody cytoplasmic domain - 131 308 K.O.

References for VGAT - 131 308