Tailor-made Antibodies
and Tools for Live Science
Home|||||||Technical Support|

VGAT antibody cytoplasmic domain - 131 011C2

VGAT or VIAAT is the GABA and glycine transporter in the membrane of synaptic vesicles
Monoclonal mouse purified IgG
Cat. No.: 131 011C2
Amount: 100 µg
Price: $465.00
Cat. No. 131 011C2 100 µg purified IgG, lyophilized. Albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications WB: N/A
IP: N/A
ICC: 1 : 100 up to 1 : 1000 gallery  
IHC: not tested yet
IHC-P/FFPE: not tested yet
Label Cyanine 2
Clone 117G4
Subtype IgG3 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 75 to 87 from rat VGAT (UniProt Id: O35458)
Epitop Epitop: AA 75 to 87 from rat VGAT (UniProt Id: O35458)
Reactivity Reacts with: human (Q9H598), rat (O35458), mouse (O35633), Guinea pig, monkey.
Other species not tested yet.
Specificity Specific for mammalian VGAT. K.O.
Matching control protein/peptide 131-0P
Data sheet 131_011c2.pdf

References for VGAT - 131 011C2

Human Striatal Dopaminergic and Regional Serotonergic Synaptic Degeneration with Lewy Body Disease and Inheritance of APOE ε4.
Postupna N, Latimer CS, Larson EB, Sherfield E, Paladin J, Shively CA, Jorgensen MJ, Andrews RN, Kaplan JR, Crane PK, Montine KS, et al.
The American journal of pathology (2017) 1874: 884-895. 131 011C2 FACS; tested species: human
Cat. No.: 131 011C2
Quantity: 100 µg
Price: $465.00
Human Striatal Dopaminergic and Regional Serotonergic Synaptic Degeneration with Lewy Body Disease and Inheritance of APOE ε4.
Postupna N, Latimer CS, Larson EB, Sherfield E, Paladin J, Shively CA, Jorgensen MJ, Andrews RN, Kaplan JR, Crane PK, Montine KS, et al.
The American journal of pathology (2017) 1874: 884-895. 131 011C2 FACS; tested species: human
Background
The vesicular GABA transporter VGAT is responsible for uptake and storage of GABA and glycine by synaptic vesicles in the central nervous system. For this reason it is frequently referred to as the vesicular inhibitory aminoacid transporter VIAAT. It is different from the plasma membrane transporters in that it is driven by a proton electrochemical gradient across the vesicle membrane. So far, only one isoform is known. VGAT is currently the best marker for inhibitory nerve terminals.
Protocols