Tailor-made Antibodies
and Tools for Life Science

VGAT antibody - 131 009

VGAT or VIAAT is the GABA and glycine transporter in the membrane of synaptic vesicles
Chicken monoclonal recombinant IgY
Cat. No.: 131 009
Amount: 50 µg
Price: $420.00
Cat. No. 131 009 50 µg purified recombinant IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 2000 (AP-staining) (see remarks) gallery  
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 500 up to 1 : 1000 gallery  
Clone Ch117G4
Subtype IgY (λ light chain)
Immunogen Synthetic peptide corresponding to residues near the amino terminus of rat VGAT (UniProt Id: O35458)
Reactivity Reacts with: human (Q9H598), rat (O35458), mouse (O35633), Guinea pig, ape.
Other species not tested yet.
Matching control protein/peptide 131-0P
Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 117G4. The constant regions of the heavy and light chains have been replaced with chicken specific sequences. Therefore, the antibody can be used with standard anti-chicken secondary reagents. The antibody has been expressed in mammalian cells.
WB: VGAT aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

Data sheet 131_009.pdf
Cat. No.: 131 009
Amount: 50 µg
Price: $420.00