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Synaptotagmin 1 lumenal domain - 105-10P

Ca2+-sensor on synaptic vesicles, triggers neurotransmitter release
control peptide
Cat. No.: 105-10P
Amount: 100 µg
Price: $90.00
Cat. No. 105-10P 100 µg peptide, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Control peptides should be stored at -20°C when still lyophilized!
Immunogen Synthetic peptide corresponding to AA 1 to 8 from mouse Synaptotagmin1 (UniProt Id: P46096)
Recommended dilution Optimal concentrations should be determined by the end-user.
Matching antibodies 105 102, 105 103, 105 103BT, 105 103C2, 105 103C3, 105 103C5, 105 103CpH, 105 104, 105 105, 105 106, 105 103AF
Remarks

This control peptide consists of the synthetic peptide MVSASRPE (aa 1-8 in mouse synaptotagmin 1) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.

Data sheet 105-10p.pdf
Cat. No.: 105-10P
Quantity: 100 µg
Price: $90.00
Background
Synaptotagmin 1 also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca2+-binding C2-domains.
Ca2+-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca2+-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.
Protocols