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Home > Products > 105 103

Synaptotagmin1 antibody luminal domain - 105 103 K.O.

Synaptotagmin1 is a Ca2+-sensor on synaptic vesicles that triggers neurotransmitter release
Rabbit polyclonal purified antibody
Cat. No.: 105 103
Amount: 50 µg
Price: $465.00
Cat. No. 105 103 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 100 up to 1 : 500 (see remarks) gallery  
IHC: 1 : 500 (rat only) gallery  
IHC-P: not tested yet
Immunogen Synthetic peptide corresponding to AA 1 to 8 from mouse Synaptotagmin1 (UniProt Id: P46096)
Reactivity Reacts with: rat (P21707), mouse (P46096).
Other species not tested yet.

For unknown reasons antibodies raised against the luminal N-terminus of Synaptotagmin 1 show a strong preference for the rat protein.
Specificity K.O. validated PubMed: 31501440
Matching control protein/peptide 105-10P
Remarks

ICC: This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures.
Data sheet 105_103.pdf

References for Synaptotagmin1 - 105 103

Mouse-derived Synaptosomes Trypsin Cleavage Assay to Characterize Synaptic Protein Sub-localization.
Shergill JK, Tehran DA
Bio-protocol (2025) 152: e5164. 105 103 WB; tested species: mouse
Monitoring of activity-driven trafficking of endogenous synaptic proteins through proximity labeling.
Pascual-Caro C, de Juan-Sanz J
PLoS biology (2024) 2210: e3002860. 105 103 WB; tested species: rat
Synaptotagmin 2 is ectopically overexpressed in excitatory presynapses of a widely used CaMKΙΙα-Cre mouse line.
Matsuura K, Mohamed HMA, Youssef MMM, Yoshida Y, Yamamoto T
iScience (2022) 258: 104692. 105 103 WB; tested species: mouse
Paroxysmal and cognitive phenotypes in Prrt2 mutant mice.
Robertson L, Featherby T, Howell S, Hughes J, Thomas P
Genes, brain, and behavior (2019) : e12566. 105 103 WB; tested species: mouse
Synaptophysin sustains presynaptic performance by preserving vesicular synaptobrevin-II levels.
Kokotos AC, Harper CB, Marland JRK, Smillie KJ, Cousin MA, Gordon SL
Journal of neurochemistry (2019) : . 105 103 WB, ICC; tested species: mouse
Synaptotagmin 1 clamps synaptic vesicle fusion in mammalian neurons independent of complexin.
Courtney NA, Bao H, Briguglio JS, Chapman ER
Nature communications (2019) 101: 4076. 105 103 WB; KO verified; tested species: mouse
Elevated synaptic vesicle release probability in synaptophysin/gyrin family quadruple knockouts.
Raja MK, Preobraschenski J, Del Olmo-Cabrera S, Martinez-Turrillas R, Jahn R, Perez-Otano I, Wesseling JF
eLife (2019) 8: . 105 103 WB; tested species: mouse
Expression and secretion of synaptic proteins during stem cell differentiation to cortical neurons.
Nazir FH, Becker B, Brinkmalm A, Höglund K, Sandelius Å, Bergström P, Satir TM, Öhrfelt A, Blennow K, Agholme L, Zetterberg H, et al.
Neurochemistry international (2018) : . 105 103 WB, ICC; tested species: human
Simultaneous monitoring of presynaptic transmitter release and postsynaptic receptor trafficking reveals an enhancement of presynaptic activity in metabotropic glutamate receptor-mediated long-term depression.
Xu W, Tse YC, Dobie FA, Baudry M, Craig AM, Wong TP, Wang YT
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3313: 5867-5877. 105 103 WB
Cat. No.: 105 103
Amount: 50 µg
Price: $465.00
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