Synaptic Systems - Synaptotagmin1

Cat. No.: 105 103C3

Amount: 50 µg

Price: $480.00

Cat. No. 105 103C3	50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with Cyanine 3 Fluorescence labeled antibodies conjugated to Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy. Cyanine 2: λ_ex 492 nm / λ_em 508 nm Sulfo-Cyanine 3: λ_ex 554 nm / λ_em 568 nm Sulfo-Cyanine 5: λ_ex 649 nm / λ_em 667 nm Cyanine 5 dyes are well suited for STORM high resolution microscopy. Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates. . Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	WB: N/A IP: N/A ICC: 1 : 100 up to 1 : 500 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet
Label	Sulfo-Cyanine 3
Immunogen	Synthetic peptide corresponding to AA 1 to 8 from mouse Synaptotagmin1 (UniProt Id: P46096)
Reactivity	Reacts with: rat (P21707), mouse (P46096). Other species not tested yet. For unknown reasons antibodies raised against the luminal N-terminus of Synaptotagmin 1 show a strong preference for the rat protein.
Matching control protein/peptide	105-10P
Remarks	ICC: This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures.
Data sheet	105_103c3.pdf

References for Synaptotagmin1 - 105 103C3

Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum.
Kuijpers M, Kochlamazashvili G, Stumpf A, Puchkov D, Swaminathan A, Lucht MT, Krause E, Maritzen T, Schmitz D, Haucke V
Neuron (2021) 1092: 299-313.e9. 105 103C3 ICC; tested species: mouse

Endogenous β-neurexins on axons and within synapses show regulated dynamic behavior.
Klatt O, Repetto D, Brockhaus J, Reissner C, El Khallouqi A, Rohlmann A, Heine M, Missler M
Cell reports (2021) 3511: 109266. 105 103C3 ICC, UPTAKE; tested species: mouse

Regulated Dynamic Trafficking of Neurexins Inside and Outside of Synaptic Terminals.
Neupert C, Schneider R, Klatt O, Reissner C, Repetto D, Biermann B, Niesmann K, Missler M, Heine M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3540: 13629-47. 105 103C3 ICC

The SNARE protein vti1a functions in dense-core vesicle biogenesis.
Walter AM, Kurps J, de Wit H, Schöning S, Toft-Bertelsen TL, Lauks J, Ziomkiewicz I, Weiss AN, Schulz A, Fischer von Mollard G, Verhage M, et al.
The EMBO journal (2014) 3315: 1681-97. 105 103C3 ICC; tested species: mouse

An activity-dependent determinant of synapse elimination in the mammalian brain.
Yasuda M, Nagappan-Chettiar S, Johnson-Venkatesh EM, Umemori H
Neuron (2021) 1098: 1333-1349.e6. 105 103C3 IHC; tested species: mouse

Bassoon controls synaptic vesicle release via regulation of presynaptic phosphorylation and cAMP.
Montenegro-Venegas C, Guhathakurta D, Pina-Fernandez E, Andres-Alonso M, Plattner F, Gundelfinger ED, Fejtova A
EMBO reports (2022) 238: e53659. 105 103C3 UPTAKE; tested species: mouse

Aβ1-16 controls synaptic vesicle pools at excitatory synapses via cholinergic modulation of synapsin phosphorylation.
Anni D, Weiss EM, Guhathakurta D, Akdas YE, Klueva J, Zeitler S, Andres-Alonso M, Huth T, Fejtova A
Cellular and molecular life sciences : CMLS (2021) : . 105 103C3 UPTAKE; tested species: rat

CtBP1-Mediated Membrane Fission Contributes to Effective Recycling of Synaptic Vesicles.
Ivanova D, Imig C, Camacho M, Reinhold A, Guhathakurta D, Montenegro-Venegas C, Cousin MA, Gundelfinger ED, Rosenmund C, Cooper B, Fejtova A, et al.
Cell reports (2020) 307: 2444-2459.e7. 105 103C3 UPTAKE; tested species: mouse

Parkin contributes to synaptic vesicle autophagy in Bassoon-deficient mice.
Hoffmann-Conaway S, Brockmann MM, Schneider K, Annamneedi A, Rahman KA, Bruns C, Textoris-Taube K, Trimbuch T, Smalla KH, Rosenmund C, Gundelfinger ED, et al.
eLife (2020) 9: . 105 103C3 UPTAKE; tested species: mouse

Fluoxetine Suppresses Glutamate- and GABA-Mediated Neurotransmission by Altering SNARE Complex.
Lazarevic V, Mantas I, Flais I, Svenningsson P
International journal of molecular sciences (2019) 2017: . 105 103C3 UPTAKE; tested species: rat

A Unique Homeostatic Signaling Pathway Links Synaptic Inactivity to Postsynaptic mTORC1.
Henry FE, Wang X, Serrano D, Perez AS, Carruthers CJL, Stuenkel EL, Sutton MA
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) 389: 2207-2225. 105 103C3 UPTAKE; tested species: rat

Aberrant neuronal activity-induced signaling and gene expression in a mouse model of RASopathy.
Altmüller F, Pothula S, Annamneedi A, Nakhaei-Rad S, Montenegro-Venegas C, Pina-Fernández E, Marini C, Santos M, Schanze D, Montag D, Ahmadian MR, et al.
PLoS genetics (2017) 133: e1006684. 105 103C3 UPTAKE

Structure of excitatory synapses and GABAA receptor localization at inhibitory synapses are regulated by neuroplastin-65.
Herrera-Molina R, Sarto-Jackson I, Montenegro-Venegas C, Heine M, Smalla KH, Seidenbecher CI, Beesley PW, Gundelfinger ED, Montag D
The Journal of biological chemistry (2014) 28913: 8973-88. 105 103C3 UPTAKE; tested species: mouse

Retrograde changes in presynaptic function driven by dendritic mTORC1.
Henry FE, McCartney AJ, Neely R, Perez AS, Carruthers CJ, Stuenkel EL, Inoki K, Sutton MA
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3248: 17128-42. 105 103C3 UPTAKE; tested species: rat

Cat. No.: 105 103C3

Amount: 50 µg

Price: $480.00

ICC

Background

Synaptotagmin 1 also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca²⁺-binding C2-domains.
Ca²⁺-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca²⁺-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.

1 ) Geppert M, Annual review of neuroscience (1998) 21: 75-95.
2 ) Jahn R, Annual review of neuroscience (1994) 17: 219-46.
3 ) Südhof TC, Nature (1995) 3756533: 645-53.
4 ) Geppert M, Cell (1994) 794: 717-27.
5 ) Brose N, Science (New York, N.Y.) (1992) 2565059: 1021-5.

Protocols

ICC

OTHER

Preabsorbtion

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Synaptotagmin1 antibody luminal domain - 105 103C3

References for Synaptotagmin1 - 105 103C3