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Cat. No. 105 103C2 |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with Cyanine 2. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
Applications |
IP: N/A ICC: 1 : 100 up to 1 : 500 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet |
Label | Cyanine 2 |
Immunogen | Synthetic peptide corresponding to AA 1 to 8 from mouse Synaptotagmin1 (UniProt Id: P46096) |
Reactivity |
Reacts with: rat (P21707), mouse (P46096). Other species not tested yet. For unknown reasons antibodies raised against the luminal N-terminus of Synaptotagmin 1 show a strong preference for the rat protein. |
Matching control protein/peptide | 105-10P |
Remarks |
ICC: This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures. |
Data sheet | 105_103c2.pdf |
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