Cat. No.: 105 103C2
Amount: 50 µg
|Cat. No. 105 103C2||
50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with Sulfo-Cyanine 2. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
ICC: 1 : 100 up to 1 : 500 gallery
IHC: not tested yet
IHC-P/FFPE: not tested yet
|Immunogen||Synthetic peptide corresponding to AA 1 to 8 from mouse Synaptotagmin1 (UniProt Id: P46096)|
Reacts with: rat (P21707), mouse (P46096).
Other species not tested yet.
For unknown reasons antibodies raised against the luminal N-terminus of Synaptotagmin 1 show a strong preference for the rat protein.
|Specificity||Specific for synaptotagmin 1, no cross-reactivity to synaptotagmin 2.|
|Matching control protein/peptide||105-10P|
This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures.