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Synaptotagmin 1 antibody luminal domain - 105 103C2

Synaptotagmin1 is a Ca2+-sensor on synaptic vesicles that triggers neurotransmitter release
Rabbit polyclonal purified antibody
Cat. No.: 105 103C2
Amount: 50 µg
Price: $475.00
Cat. No. 105 103C2 50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with Sulfo-Cyanine 2. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 100 up to 1 : 500 (see remarks) gallery  
IHC: not tested yet
IHC-P: not tested yet
Label Sulfo-Cyanine 2
Immunogen Synthetic peptide corresponding to AA 1 to 8 from mouse Synaptotagmin1 (UniProt Id: P46096)
Reactivity Reacts with: rat (P21707), mouse (P46096).
Other species not tested yet.
For unknown reasons antibodies raised against the luminal N-terminus of Synaptotagmin 1 show a strong preference for the rat protein.
Specificity Specific for synaptotagmin 1, no cross-reactivity to synaptotagmin 2.
Matching control protein/peptide 105-10P
Remarks

ICC: This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures.

Data sheet 105_103c2.pdf

References for Synaptotagmin 1 - 105 103C2

Extracellular matrix remodeling through endocytosis and resurfacing of Tenascin-R.
Dankovich TM, Kaushik R, Olsthoorn LHM, Petersen GC, Giro PE, Kluever V, Agüi-Gonzalez P, Grewe K, Bao G, Beuermann S, Hadi HA, et al.
Nature communications (2021) 121: 7129. 105 103C2 WB, ICC; KD verified; tested species: rat
Extracellular matrix remodeling through endocytosis and resurfacing of Tenascin-R.
Dankovich TM, Kaushik R, Olsthoorn LHM, Petersen GC, Giro PE, Kluever V, Agüi-Gonzalez P, Grewe K, Bao G, Beuermann S, Hadi HA, et al.
Nature communications (2021) 121: 7129. 105 103C2 WB, ICC; KD verified; tested species: rat
Cat. No.: 105 103C2
Amount: 50 µg
Price: $475.00
Extracellular matrix remodeling through endocytosis and resurfacing of Tenascin-R.
Dankovich TM, Kaushik R, Olsthoorn LHM, Petersen GC, Giro PE, Kluever V, Agüi-Gonzalez P, Grewe K, Bao G, Beuermann S, Hadi HA, et al.
Nature communications (2021) 121: 7129. 105 103C2 WB, ICC; KD verified; tested species: rat
Extracellular matrix remodeling through endocytosis and resurfacing of Tenascin-R.
Dankovich TM, Kaushik R, Olsthoorn LHM, Petersen GC, Giro PE, Kluever V, Agüi-Gonzalez P, Grewe K, Bao G, Beuermann S, Hadi HA, et al.
Nature communications (2021) 121: 7129. 105 103C2 WB, ICC; KD verified; tested species: rat
Background
Synaptotagmin 1 also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca2+-binding C2-domains.
Ca2+-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca2+-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.
Protocols