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Synaptotagmin1 antibody luminal domain - 105 103BT

Synaptotagmin1 is a Ca2+-sensor on synaptic vesicles that triggers neurotransmitter release
Rabbit polyclonal purified antibody
Cat. No.: 105 103BT
Amount: 50 µg
Price: $480.00
Cat. No. 105 103BT 50 µg specific antibody, lyophilized. Affinity purified with the immunogen, biotin-labeled. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 100 up to 1 : 500 (see remarks) gallery  
IHC: not tested yet
IHC-P: not tested yet
Label biotin
Immunogen Synthetic peptide corresponding to AA 1 to 8 from mouse Synaptotagmin1 (UniProt Id: P46096)
Reactivity Reacts with: rat (P21707), mouse (P46096).
Other species not tested yet.
For unknown reasons antibodies raised against the luminal N-terminus of Synaptotagmin 1 show a strong preference for the rat protein.
Matching control protein/peptide 105-10P
Remarks

ICC: This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures.

Data sheet 105_103bt.pdf

References for Synaptotagmin1 - 105 103BT

Single Calcium Channel Nanodomains Drive Presynaptic Calcium Entry at Lamprey Reticulospinal Presynaptic Terminals.
Ramachandran S, Rodgriguez S, Potcoava M, Alford S
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) 4212: 2385-2403. 105 103BT UPTAKE
Real-time three-dimensional tracking of single vesicles reveals abnormal motion and pools of synaptic vesicles in neurons of Huntington's disease mice.
Chen S, Yoo H, Li CH, Park C, Park G, Tan LY, Jung S, Park H
iScience (2021) 2410: 103181. 105 103BT UPTAKE; tested species: mouse
Cat. No.: 105 103BT
Amount: 50 µg
Price: $480.00
Single Calcium Channel Nanodomains Drive Presynaptic Calcium Entry at Lamprey Reticulospinal Presynaptic Terminals.
Ramachandran S, Rodgriguez S, Potcoava M, Alford S
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) 4212: 2385-2403. 105 103BT UPTAKE
Real-time three-dimensional tracking of single vesicles reveals abnormal motion and pools of synaptic vesicles in neurons of Huntington's disease mice.
Chen S, Yoo H, Li CH, Park C, Park G, Tan LY, Jung S, Park H
iScience (2021) 2410: 103181. 105 103BT UPTAKE; tested species: mouse
Background
Synaptotagmin 1 also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca2+-binding C2-domains.
Ca2+-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca2+-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.