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Synaptotagmin 1 lumenal domain - 105 102

Ca2+-sensor on synaptic vesicles, triggers neurotransmitter release
Polyclonal rabbit antiserum
Cat. No.: 105 102
Amount: 200 µl
Price: 350.00 €
Cat. No. 105 102 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 100 gallery  
IHC: 1 : 500 gallery  
IHC-P/FFPE: not tested yet
Immunogen Synthetic peptide corresponding to AA 1 to 8 from mouse Synaptotagmin1 (UniProt Id: P46096)
Reactivity Reacts with: rat (P21707), mouse (P46096).
Other species not tested yet.
For unknown reasons antibodies raised against the luminal N-terminus of Synaptotagmin 1 show a strong preference for the rat protein.
Specificity Specific for synaptotagmin 1, no cross-reactivity to synaptotagmin 2.
Matching control protein/peptide 105-10P
Remarks This antibody can be used for labeling of recycling synaptic vesicles by adding to living neurons or as a marker for exocytosis in isolated nerve terminals.
Data sheet 105_102.pdf

References for Synaptotagmin 1 - 105 102

Synaptogyrin-3 Mediates Presynaptic Dysfunction Induced by Tau.
McInnes J, Wierda K, Snellinx A, Bounti L, Wang YC, Stancu IC, Apóstolo N, Gevaert K, Dewachter I, Spires-Jones TL, De Strooper B, et al.
Neuron (2018) 974: 823-835.e8. 105 102 WB; tested species: mouse
Aberrant function and structure of retinal ribbon synapses in the absence of complexin 3 and complexin 4.
Reim K, Regus-Leidig H, Ammermüller J, El-Kordi A, Radyushkin K, Ehrenreich H, Brandstätter JH, Brose N
Journal of cell science (2009) 122Pt 9: 1352-61. 105 102 WB; tested species: mouse
Unique luminal localization of VGAT-C terminus allows for selective labeling of active cortical GABAergic synapses.
Martens H, Weston MC, Boulland JL, Grønborg M, Grosche J, Kacza J, Hoffmann A, Matteoli M, Takamori S, Harkany T, Chaudhry FA, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2008) 2849: 13125-31. 105 102 WB; tested species: rat
Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
The EMBO journal (2018) : . 105 102 ICC; tested species: rat
Nanoscale Structural Plasticity of the Active Zone Matrix Modulates Presynaptic Function.
Glebov OO, Jackson RE, Winterflood CM, Owen DM, Barker EA, Doherty P, Ewers H, Burrone J
Cell reports (2017) 1811: 2715-2728. 105 102 ICC
Afadin regulates puncta adherentia junction formation and presynaptic differentiation in hippocampal neurons.
Toyoshima D, Mandai K, Maruo T, Supriyanto I, Togashi H, Inoue T, Mori M, Takai Y
PloS one (2014) 92: e89763. 105 102 ICC; tested species: mouse
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 105 102 ICC, IHC; tested species: mouse
A new probe for super-resolution imaging of membranes elucidates trafficking pathways.
Revelo NH, Kamin D, Truckenbrodt S, Wong AB, Reuter-Jessen K, Reisinger E, Moser T, Rizzoli SO
The Journal of cell biology (2014) 2054: 591-606. 105 102 ICC
The neural cell adhesion molecule promotes maturation of the presynaptic endocytotic machinery by switching synaptic vesicle recycling from adaptor protein 3 (AP-3)- to AP-2-dependent mechanisms.
Shetty A, Sytnyk V, Leshchyns'ka I, Puchkov D, Haucke V, Schachner M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3342: 16828-45. 105 102 ICC; tested species: mouse
Unique versus Redundant Functions of Neuroligin Genes in Shaping Excitatory and Inhibitory Synapse Properties.
Chanda S, Hale WD, Zhang B, Wernig M, Südhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3729: 6816-6836. 105 102 IHC; tested species: mouse
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 105 102 ICC, IHC; tested species: mouse
Expression of Ttyh1, a member of the Tweety family in neurons in vitro and in vivo and its potential role in brain pathology.
Stefaniuk M, Swiech L, Dzwonek J, Lukasiuk K
Journal of neurochemistry (2010) 1155: 1183-94. 105 102 IHC; tested species: rat
A novel non-canonical Notch signaling regulates expression of synaptic vesicle proteins in excitatory neurons.
Hayashi Y, Nishimune H, Hozumi K, Saga Y, Harada A, Yuzaki M, Iwatsubo T, Kopan R, Tomita T
Scientific reports (2016) 6: 23969. 105 102 UPTAKE; tested species: mouse
Cat. No.: 105 102
Quantity: 200 µl
Price: 350.00 €
Synaptogyrin-3 Mediates Presynaptic Dysfunction Induced by Tau.
McInnes J, Wierda K, Snellinx A, Bounti L, Wang YC, Stancu IC, Apóstolo N, Gevaert K, Dewachter I, Spires-Jones TL, De Strooper B, et al.
Neuron (2018) 974: 823-835.e8. 105 102 WB; tested species: mouse
Aberrant function and structure of retinal ribbon synapses in the absence of complexin 3 and complexin 4.
Reim K, Regus-Leidig H, Ammermüller J, El-Kordi A, Radyushkin K, Ehrenreich H, Brandstätter JH, Brose N
Journal of cell science (2009) 122Pt 9: 1352-61. 105 102 WB; tested species: mouse
Unique luminal localization of VGAT-C terminus allows for selective labeling of active cortical GABAergic synapses.
Martens H, Weston MC, Boulland JL, Grønborg M, Grosche J, Kacza J, Hoffmann A, Matteoli M, Takamori S, Harkany T, Chaudhry FA, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2008) 2849: 13125-31. 105 102 WB; tested species: rat
Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
The EMBO journal (2018) : . 105 102 ICC; tested species: rat
Nanoscale Structural Plasticity of the Active Zone Matrix Modulates Presynaptic Function.
Glebov OO, Jackson RE, Winterflood CM, Owen DM, Barker EA, Doherty P, Ewers H, Burrone J
Cell reports (2017) 1811: 2715-2728. 105 102 ICC
Afadin regulates puncta adherentia junction formation and presynaptic differentiation in hippocampal neurons.
Toyoshima D, Mandai K, Maruo T, Supriyanto I, Togashi H, Inoue T, Mori M, Takai Y
PloS one (2014) 92: e89763. 105 102 ICC; tested species: mouse
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 105 102 ICC, IHC; tested species: mouse
A new probe for super-resolution imaging of membranes elucidates trafficking pathways.
Revelo NH, Kamin D, Truckenbrodt S, Wong AB, Reuter-Jessen K, Reisinger E, Moser T, Rizzoli SO
The Journal of cell biology (2014) 2054: 591-606. 105 102 ICC
The neural cell adhesion molecule promotes maturation of the presynaptic endocytotic machinery by switching synaptic vesicle recycling from adaptor protein 3 (AP-3)- to AP-2-dependent mechanisms.
Shetty A, Sytnyk V, Leshchyns'ka I, Puchkov D, Haucke V, Schachner M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3342: 16828-45. 105 102 ICC; tested species: mouse
Unique versus Redundant Functions of Neuroligin Genes in Shaping Excitatory and Inhibitory Synapse Properties.
Chanda S, Hale WD, Zhang B, Wernig M, Südhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3729: 6816-6836. 105 102 IHC; tested species: mouse
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 105 102 ICC, IHC; tested species: mouse
Expression of Ttyh1, a member of the Tweety family in neurons in vitro and in vivo and its potential role in brain pathology.
Stefaniuk M, Swiech L, Dzwonek J, Lukasiuk K
Journal of neurochemistry (2010) 1155: 1183-94. 105 102 IHC; tested species: rat
A novel non-canonical Notch signaling regulates expression of synaptic vesicle proteins in excitatory neurons.
Hayashi Y, Nishimune H, Hozumi K, Saga Y, Harada A, Yuzaki M, Iwatsubo T, Kopan R, Tomita T
Scientific reports (2016) 6: 23969. 105 102 UPTAKE; tested species: mouse
Background
Synaptotagmin 1 also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca2+-binding C2-domains.
Ca2+-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca2+-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.