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Synaptotagmin 1 lumenal domain - 105 104

Ca2+-sensor on synaptic vesicles, triggers neurotransmitter release
Polyclonal Guinea pig antiserum
Cat. No.: 105 104
Amount: 100 µl
Price: $350.00
Cat. No. 105 104 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Applications WB: 1 : 2000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 5000 gallery  
IHC: 1 : 500 gallery  
IHC-P/FFPE: not tested yet
Immunogen Synthetic peptide corresponding to AA 1 to 8 from mouse Synaptotagmin1 (UniProt Id: P46096)
Reactivity Reacts with: rat (P21707).
Other species not tested yet.
For unknown reasons antibodies raised against the luminal N-terminus of Synaptotagmin 1 show a strong preference for the rat protein.
Specificity Specific for synaptotagmin 1, no cross-reactivity to synaptotagmin 2.
Matching control protein/peptide 105-10P
Remarks

This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures.

Data sheet 105_104.pdf

References for Synaptotagmin 1 - 105 104

Neural Cell Adhesion Molecule 2 (NCAM2)-Induced c-Src-Dependent Propagation of Submembrane Ca2+ Spikes Along Dendrites Inhibits Synapse Maturation.
Sheng L, Leshchyns'ka I, Sytnyk V
Cerebral cortex (New York, N.Y. : 1991) (2018) : . 105 104 ICC; tested species: mouse
Neuronal profilin isoforms are addressed by different signalling pathways.
Murk K, Wittenmayer N, Michaelsen-Preusse K, Dresbach T, Schoenenberger CA, Korte M, Jockusch BM, Rothkegel M
PloS one (2012) 73: e34167. 105 104 ICC; tested species: rat
Cat. No.: 105 104
Quantity: 100 µl
Price: $350.00
Neural Cell Adhesion Molecule 2 (NCAM2)-Induced c-Src-Dependent Propagation of Submembrane Ca2+ Spikes Along Dendrites Inhibits Synapse Maturation.
Sheng L, Leshchyns'ka I, Sytnyk V
Cerebral cortex (New York, N.Y. : 1991) (2018) : . 105 104 ICC; tested species: mouse
Neuronal profilin isoforms are addressed by different signalling pathways.
Murk K, Wittenmayer N, Michaelsen-Preusse K, Dresbach T, Schoenenberger CA, Korte M, Jockusch BM, Rothkegel M
PloS one (2012) 73: e34167. 105 104 ICC; tested species: rat
Background
Synaptotagmin 1 also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca2+-binding C2-domains.
Ca2+-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca2+-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.