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FluoTags are camelid single domain antibodies consisting only of one antigen binding site of a Alpaka heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.
|Cat. No. N0702-SC3-S||
500µl 5µM camelid sdAb in buffered saline, 50% glycerol, 0.09% sodium azide
Up to three months: -20°C
Up to 12 months: -80°C or below
Protect form light!
ICC: 1 : 500
IHC: 1 : 500
|Label||Sulfo-Cyanine 3, two fluorophores coupled to one FluoTag|
Reacts with: chicken.
No signal: mouse, rabbit, Guinea pig, donkey, rat, goat, pig, horse, cow, human.
Other species not tested yet.
|Specificity||Recognizes native chicken IgY. It does not cross-react to mouse, rabbit, Guinea pig, rat, donkey, goat, pig, horse, cow or human IgGs.|
IgY is the major immunoglobulin found in chicken eggs. FluoTag®-X2 anti-chicken is a species-specific FluoTag®-X2 directed against immunoglobulin IgY from chicken.
In FluoTag®-X2, two fluorophore molecules are coupled site-specifically to one individual FluoTag® molecule. Therefore, the reagent targets four fluorophores to your primary chicken antibody.
Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization.