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Abeta 38 - 218 421

A major component of neuritic plaques in Alzheimer's disease
Monoclonal mouse purified IgG
Cat. No.: 218 421
Amount: 100 µg
Price: 415.00 €
Cat. No. 218 421 100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (see remarks) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 (see remarks) gallery  
IHC-P/FFPE: 1 : 200 (see remarks) gallery  
Clone 326F12
Subtype IgG2b (κ light chain)
Immunogen Synthetic peptide corresponding to AA 33 to 38 from human Abeta38 (UniProt Id: P05067)
Epitop Epitop: AA 33 to 38 from human Abeta38 (UniProt Id: P05067)
Reactivity Reacts with: human.
Other species not tested yet.
Specificity Specific for Abeta 38
Remarks

WB: Detects specifically 20 ng of purified Abeta 38. Complex samples like brain extracts still have to be tested. Boil membrane after blotting for 3min.

IHC: Antigen retrieval with formic acid is required.

IHC-P: Antigen retrieval with formic acid is required.

Data sheet 218_421.pdf

References for Abeta 38 - 218 421

Deposition of C-terminally truncated Aβ species Aβ37 and Aβ39 in Alzheimer's disease and transgenic mouse models.
Reinert J, Richard BC, Klafki HW, Friedrich B, Bayer TA, Wiltfang J, Kovacs GG, Ingelsson M, Lannfelt L, Paetau A, Bergquist J, et al.
Acta neuropathologica communications (2016) 4: 24. 218 421 IHC-P
Cat. No.: 218 421
Quantity: 100 µg
Price: 415.00 €
Deposition of C-terminally truncated Aβ species Aβ37 and Aβ39 in Alzheimer's disease and transgenic mouse models.
Reinert J, Richard BC, Klafki HW, Friedrich B, Bayer TA, Wiltfang J, Kovacs GG, Ingelsson M, Lannfelt L, Paetau A, Bergquist J, et al.
Acta neuropathologica communications (2016) 4: 24. 218 421 IHC-P
Background

Amyloid deposits, also called plaques, of Alzheimer's patients consist of several protein components like the amyloid beta-peptides (Abeta, Aβ) 1-40/42/43 and additional C- and N-terminally modified fragments of Abeta as for instance Abeta pE3 and Abeta pE11.
An additional Abeta variant, Abeta 38, is more soluble compared to other Abeta species and is not found in plaques of sporadic Alzheimer´s cases. However, it is detected in the blood-vessel walls of a subset of patients with severe cerebral amyloid angiopathy. It especially accumulates in brains of patients carrying mutations in the Abeta coding region.
Cleavage of amyloid precursor protein APP by β- and γ- secretases results in the generation of the Aβ (βA4)peptide, whereas α-secretase cleaves within the Aβ sequence and prevents the formation of Abeta from APP.