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Abeta 38 - 218 411

A major component of neuritic plaques in Alzheimer's disease
Monoclonal mouse purified IgG
Cat. No.: 218 411
Amount: 100 µg
Price: 415.00 €
Cat. No. 218 411 100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (see remarks) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 (see remarks) gallery  
IHC-P/FFPE: 1 : 250 up to 1 : 2000 (see remarks) gallery  
ELISA: yes , suitable as detector and capture antibody . gallery  
Clone 67B8
Subtype IgG2a
Immunogen Synthetic peptide corresponding to AA 33 to 38 from human Abeta38 (UniProt Id: P05067)
Epitop Epitop: AA 33 to 38 from human Abeta38 (UniProt Id: P05067)
Reactivity Reacts with: human (P05067).
Other species not tested yet.
Specificity Specific for Abeta 38
Remarks

WB: Detects specifically 1 ng of purified Abeta 38. Complex samples like brain extracts still have to be tested. Boil membrane after blotting for 3min.

IHC: Antigen retrieval with formic acid is required.

IHC-P: Antigen retrieval with formic acid is required.

Data sheet 218_411.pdf

References for Abeta 38 - 218 411

A cellular complex of BACE1 and γ-secretase sequentially generates Aβ from its full-length precursor.
Liu L, Ding L, Rovere M, Wolfe MS, Selkoe DJ
The Journal of cell biology (2019) 2182: 644-663. 218 411 ELISA; tested species: human
Multiple BACE1 inhibitors abnormally increase the BACE1 protein level in neurons by prolonging its half-life.
Liu L, Lauro BM, Ding L, Rovere M, Wolfe MS, Selkoe DJ
Alzheimer's & dementia : the journal of the Alzheimer's Association (2019) : . 218 411 ELISA; tested species: human
Cat. No.: 218 411
Quantity: 100 µg
Price: 415.00 €
A cellular complex of BACE1 and γ-secretase sequentially generates Aβ from its full-length precursor.
Liu L, Ding L, Rovere M, Wolfe MS, Selkoe DJ
The Journal of cell biology (2019) 2182: 644-663. 218 411 ELISA; tested species: human
Multiple BACE1 inhibitors abnormally increase the BACE1 protein level in neurons by prolonging its half-life.
Liu L, Lauro BM, Ding L, Rovere M, Wolfe MS, Selkoe DJ
Alzheimer's & dementia : the journal of the Alzheimer's Association (2019) : . 218 411 ELISA; tested species: human
Background

Amyloid deposits, also called plaques, of Alzheimer's patients consist of several protein components like the amyloid beta-peptides (Abeta, Aβ) 1-40/42/43 and additional C- and N-terminally modified fragments of Abeta as for instance Abeta pE3 and Abeta pE11.
An additional Abeta variant, Abeta 38, is more soluble compared to other Abeta species and is not found in plaques of sporadic Alzheimer´s cases. However, it is detected in the blood-vessel walls of a subset of patients with severe cerebral amyloid angiopathy. It especially accumulates in brains of patients carrying mutations in the Abeta coding region.
Cleavage of amyloid precursor protein APP by β- and γ- secretases results in the generation of the Aβ (βA4)peptide, whereas α-secretase cleaves within the Aβ sequence and prevents the formation of Abeta from APP.