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Abeta-pE3 - 218 011BT

A major component of neuritic plaques in Alzheimer's disease
Monoclonal mouse purified IgG
Cat. No.: 218 011BT
Amount: 100 µg
Price: $465.00
Cat. No. 218 011BT 100 µg purified IgG, lyophilized, biotin-labeled. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: not tested yet
IP: not tested yet
ICC: not tested yet
IHC: 1 : 400 (see remarks)
IHC-P/FFPE: 1 : 100
ELISA: yes (see remarks)
Label biotin
Clone 2-48
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 3 to 7 from human Abeta-pE3 (UniProt Id: P05067)
Epitop Epitop: AA 3 to 7 from human Abeta-pE3 (UniProt Id: P05067)
Reactivity Reacts with: human (P05067), rat (P08592), mouse (P12023).
Other species not tested yet.
Specificity Specific for Abeta-pE3.
Remarks

IHC: Antigen retrieval with formic acid is required.
ELISA: This antibody is suitable as detector antibody for sandwich-ELISA with cat. no. 218 111 or 218 511 as capture antibody.

Data sheet 218_011bt.pdf

References for Abeta-pE3 - 218 011BT

Transplantation of bone marrow derived macrophages reduces markers of neuropathology in an APP/PS1 mouse model.
Costa-Marques L, Arnold K, Pardon MC, Leovsky C, Swarbrick S, Fabian C, Stolzing A
Translational neurodegeneration (2019) 8: 33. 218 011BT IHC; tested species: mouse
Identification of low molecular weight pyroglutamate A{beta} oligomers in Alzheimer disease: a novel tool for therapy and diagnosis.
Wirths O, Erck C, Martens H, Harmeier A, Geumann C, Jawhar S, Kumar S, Multhaup G, Walter J, Ingelsson M, Degerman-Gunnarsson M, et al.
The Journal of biological chemistry (2010) 28553: 41517-24. 218 011BT ELISA
Cat. No.: 218 011BT
Quantity: 100 µg
Price: $465.00
Transplantation of bone marrow derived macrophages reduces markers of neuropathology in an APP/PS1 mouse model.
Costa-Marques L, Arnold K, Pardon MC, Leovsky C, Swarbrick S, Fabian C, Stolzing A
Translational neurodegeneration (2019) 8: 33. 218 011BT IHC; tested species: mouse
Identification of low molecular weight pyroglutamate A{beta} oligomers in Alzheimer disease: a novel tool for therapy and diagnosis.
Wirths O, Erck C, Martens H, Harmeier A, Geumann C, Jawhar S, Kumar S, Multhaup G, Walter J, Ingelsson M, Degerman-Gunnarsson M, et al.
The Journal of biological chemistry (2010) 28553: 41517-24. 218 011BT ELISA
Background

Amyloid deposits, also called plaques, of Alzheimer's patients consist of several protein components like the amyloid beta-peptides (Abeta, ) 1-40/42 and additional C- and N-terminally truncated and modified fragments. Very abundant are the isoaspartate (isoAsp)-Abeta and pyroglutamyl (pGlu)-Abeta peptides. The latter are formed by cyclization of the N-terminal glutamate at position 3 or 11 catalyzed by glutaminyl cyclase (QC) resulting in very amyloidogenic and neurotxic variants of Abeta; Abeta-pE3 and Abeta pE11.
In contrast to extracellular plaques that do not perfectly correlate with Alzheimer´s disease intraneuronal Abeta accumulation and vascular Abeta deposits have gained more and more evidence to be among the crucial factors responsible for progressive neuron loss.