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mCLING-DY 654-labeled - 710 006DY1

A fixable membrane probe for high resolution microscopy
Cat. No.: 710 006DY1
Amount: 5 nmol
Price: 330.00 €
Cat. No. 710 006DY1

5nmol mCLING labeled with DY® 654 in 100 µl PBS (lyophilized). For reconstitution add 100 µl H2O, then aliquot and store at -80°C until use.
Reconstitute immediately upon receipt! Avoid bright light when working with the probe to minimize photo bleeching of the fluorescent dye.

Applications ICC: 1 : 75 up to 1 : 250 (0.2 - 0.7 nmol/ml) gallery  
IHC: 1 : 25 up to 1 : 50 (1 - 2 nmol/ml)
Label DY 654
Remarks

Due to the positive charge of mCLING, negatively charged coatings of cover-slips should be avoided. We recommend a positively charged coating like poly-L-lysine (PLL). mCLING is a fixable dye but paraformaldehyde alone is not able to fix this molecule sufficiently. Therefore, a mixture of 4 %paraformaldehyde (PFA) and 0.2 % glutaraldehyde is strongly advised. For detailed protocols see Revelo NH & Rizzoli SO, 2016.

Data sheet 710_006dy1.pdf
Cat. No.: 710 006DY1
Quantity: 5 nmol
Price: 330.00 €
Background
The membrane-binding fluorophore-cysteine-lysine-palmtoyl group (mCLING) is a new probe that selectively binds to the plasma membrane. It is taken up during endocytosis and, in contrast to conventional membrane dyes, remains attached to membranes after fixation and permeabilization and can therefore be combined with immunostaining and super-resolution microscopy.
mCLING was used so far in mammalian-cultured cells, yeast, bacteria, primary cultured neurons, Drosophila melanogaster larval neuromuscular junctions, and mammalian tissue.