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Spleen lysate - 601-SPL

Ready to use tissue lysates for western blots
Cat. No.: 601-SPL
Amount: 200 µg
Price: $90.00
Cat. No. 601-SPL 200 µg lysate in 1 X SDS PAGE loading buffer 'ready to use'.
Total volume: 100 µl. Concentration: 2 mg/ml.
Aliquot and store at -20°C until use.
Applications WB: yes gallery  
Documents MSDS
Loading/lane 10-30 µg
Source White Wistar
Remarks

Since several proteins aggregate after boiling, these lysates are unboiled. Boil sample before loading, if this is recommended for your protocol.

Data sheet 601-spl.pdf
Cat. No.: 601-SPL
Quantity: 200 µg
Price: $90.00
Background

In many cases the relative concentration of the protein of interest is high enough to use whole tissue lysate for Western blot testing. If the concentration is less than 0.2% of total protein it may be difficult to distinguish signals from background noise. Since a lot of proteins are localized in distinct cellular compartments, subfractioning may overcome this problem. Protein concentration has been determined with a Bradford protein quantification assay.