Synaptic Systems - alpha-Tubulin

Cat. No.: 302-21P

Amount: 100 µg

Price: $110.00

Background

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape.
Tubulin itself is a globular protein which consists of two polypeptides, α-tubulin and β-tubulin. α- and β-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter.

Assembled microtubules can be detyrosinated by a carboxypeptidaseS called vasohibins / SVBPs. Detyrosinated α-tubulin is referred to as Glu-α-tubulin and occurs for exemple in neurons. This reaction can be reverted by Tubulin tyrosine ligase (TTL) that ads a C-terminal tyrosin to Glu α-tubulin.
Another post-translational modification of α-tubulin is C-terminal polyglutamylation which is also characteristic for microtubules in neuronal cells and the mitotic spindle. A third variant of detyrosinated α-tubulin is Δ2-tubulin which lacks the C-terminal glutamic acid. It cannot be tyrosinated by TTL and is one of the dominant α-tubulin isoforms in neurons.

1 ) Erck C et al., Proc. Natl. Acad. Sci. U.S.A. (2005)
2 ) Contin MA et al., Biochem. J. (1999)
3 ) Paturle-Lafanechère L et al., J. Cell. Sci. (1994)
4 ) Ersfeld K et al., J. Cell Biol. (1993)
5 ) Rüdiger M et al., FEBS Lett. (1992)
6 ) Caron JM et al., J. Cell Biol. (1985)
7 ) Caron JM et al., Nature ()

Protocols

Other products for this target

Cat. No. 302-21P	100 µg peptide, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Control peptides should be stored at -20°C when still lyophilized!
Applications
Immunogen	Synthetic peptide corresponding to AA 419 to 435 from human α-tubulin 4A (UniProt Id: P68366)
Recommended dilution	Optimal concentrations should be determined by the end-user.
Matching antibodies	302 204, 302 211, 302 211C3, 302 411, 302 206, 302 308, 302 209
Remarks	This control peptide consists of the synthetic peptide SEAREDMAALEKDYEEV (aa 419 - 435 in human α-tubulin 4A) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet	302-21p.pdf

alpha-Tubulin peptide - 302-21P