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alpha-Tubulin - 302 411

A major cytoskeleton protein
Monoclonal mouse purified IgM
Cat. No.: 302 411
Amount: 100 µg
Price: $415.00
Cat. No. 302 411 100 µg purified IgM, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P/FFPE: not tested yet
Clone 88B6
Subtype IgM (κ light chain)
Immunogen Synthetic peptide corresponding to AA 419 to 435 from human α-tubulin 4A (UniProt Id: P68366)
Epitop Epitop: AA 419 to 485 from human α-tubulin 4A (UniProt Id: P68366)
Reactivity Reacts with: human (P68366), rat (Q5XIF6), mouse (P68368).
Other species not tested yet.
Specificity Specific for α-tubulin.
Matching control protein/peptide 302-21P
Data sheet 302_411.pdf
Cat. No.: 302 411
Quantity: 100 µg
Price: $415.00
Background

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape.
Tubulin itself is a globular protein which consists of two polypeptides, α-tubulin and β-tubulin. α- and β-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase ads a C-terminal tyrosin to monomeric α-tubulin.
Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated α-tubulin is referred to as Glu-α-tubulin. Another post-translational modification of detyrosinated α-tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle. A third variant of detyrosinated α-tubulin is Δ2-tubulin which lacks the C-terminal glutamic acid. It cannot be tyrosinated by tyrosine ligase and is one of the dominant α-tubulin isoforms in neurons.