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alpha-Tubulin antibody - 302 211

alpha-Tubulin is a major cytoskeleton protein
Mouse monoclonal purified IgG
Cat. No.: 302 211
Amount: 100 µg
Price: $415.00
Cat. No. 302 211 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P/FFPE: 1 : 500 up to 1 : 1000 gallery  
ELISA: yes (see remarks)
Clone 3A2
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 419 to 435 from human α-tubulin 4A (UniProt Id: P68366)
Epitop Epitop: AA 419 to 435 from human α-tubulin 4A (UniProt Id: P68366)
Reactivity Reacts with: human (P68366), rat, mouse, vertebrates, invertebrates, yeast.
Other species not tested yet.
Specificity Specific for α-tubulin (glu- and tyr-α-tubulin)
Matching control protein/peptide 302-21P
Remarks

ELISA: Suitable as capture antibodies for sandwich-ELISA with cat. no. 302 203 as detector antibody.

Data sheet 302_211.pdf

References for alpha-Tubulin - 302 211

Pancreas-specific SNAP23 depletion prevents pancreatitis by attenuating pathological basolateral exocytosis and formation of trypsin-activating autolysosomes.
Dolai S, Takahashi T, Qin T, Liang T, Xie L, Kang F, Miao YF, Xie H, Kang Y, Manuel J, Winter E, et al.
Autophagy (2020) : 1-14. 302 211 WB; tested species: human
SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.
Koike S, Jahn R
Nature communications (2019) 101: 1608. 302 211 WB; tested species: human
The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications.
Götzke H, Kilisch M, Martínez-Carranza M, Sograte-Idrissi S, Rajavel A, Schlichthaerle T, Engels N, Jungmann R, Stenmark P, Opazo F, Frey S, et al.
Nature communications (2019) 101: 4403. 302 211 WB; tested species: mouse
Slowing ribosome velocity restores folding and function of mutant CFTR.
Oliver KE, Rauscher R, Mijnders M, Wang W, Wolpert MJ, Maya J, Sabusap CM, Kesterson RA, Kirk KL, Rab A, Braakman I, et al.
The Journal of clinical investigation (2019) : . 302 211 WB; tested species: human
Increased expression of heme-binding protein 1 early in Alzheimer's disease is linked to neurotoxicity.
Yagensky O, Kohansal-Nodehi M, Gunaseelan S, Rabe T, Zafar S, Zerr I, Härtig W, Urlaub H, Chua JJ
eLife (2019) 8: . 302 211 WB; tested species: mouse
Truncated tau deregulates synaptic markers in rat model for human tauopathy.
Jadhav S, Katina S, Kovac A, Kazmerova Z, Novak M, Zilka N
Frontiers in cellular neuroscience (2015) 9: 24. 302 211 WB
BDNF enhances spontaneous and activity-dependent neurotransmitter release at excitatory terminals but not at inhibitory terminals in hippocampal neurons.
Shinoda Y, Ahmed S, Ramachandran B, Bharat V, Brockelt D, Altas B, Dean C
Frontiers in synaptic neuroscience (2014) 6: 27. 302 211 WB; tested species: rat
Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission.
Spangler SA, Schmitz SK, Kevenaar JT, de Graaff E, de Wit H, Demmers J, Toonen RF, Hoogenraad CC
The Journal of cell biology (2013) 2016: 915-28. 302 211 WB; tested species: rat
Exocyst sec5 regulates exocytosis of newcomer insulin granules underlying biphasic insulin secretion.
Xie L, Zhu D, Kang Y, Liang T, He Y, Gaisano HY
PloS one (2013) 87: e67561. 302 211 WB; tested species: human,mouse,rat
Doc2b is a high-affinity Ca2+ sensor for spontaneous neurotransmitter release.
Groffen AJ, Martens S, Díez Arazola R, Cornelisse LN, Lozovaya N, de Jong AP, Goriounova NA, Habets RL, Takai Y, Borst JG, Brose N, et al.
Science (New York, N.Y.) (2010) 3275973: 1614-8. 302 211 WB; tested species: mouse
Complexins facilitate neurotransmitter release at excitatory and inhibitory synapses in mammalian central nervous system.
Xue M, Stradomska A, Chen H, Brose N, Zhang W, Rosenmund C, Reim K
Proceedings of the National Academy of Sciences of the United States of America (2008) 10522: 7875-80. 302 211 WB; tested species: mouse
Circumvention of common labelling artefacts using secondary nanobodies.
Sograte-Idrissi S, Schlichthaerle T, Duque-Afonso CJ, Alevra M, Strauss S, Moser T, Jungmann R, Rizzoli SO, Opazo F
Nanoscale (2020) 1218: 10226-10239. 302 211 ICC; tested species: monkey
Intrinsic refractive index matched 3D dSTORM with two objectives: Comparison of detection techniques.
Schmidt NC, Kahms M, Hüve J, Klingauf J
Scientific reports (2018) 81: 13343. 302 211 ICC; tested species: mouse
Regulated Dynamic Trafficking of Neurexins Inside and Outside of Synaptic Terminals.
Neupert C, Schneider R, Klatt O, Reissner C, Repetto D, Biermann B, Niesmann K, Missler M, Heine M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3540: 13629-47. 302 211 ICC
Cat. No.: 302 211
Quantity: 100 µg
Price: $415.00
Pancreas-specific SNAP23 depletion prevents pancreatitis by attenuating pathological basolateral exocytosis and formation of trypsin-activating autolysosomes.
Dolai S, Takahashi T, Qin T, Liang T, Xie L, Kang F, Miao YF, Xie H, Kang Y, Manuel J, Winter E, et al.
Autophagy (2020) : 1-14. 302 211 WB; tested species: human
SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.
Koike S, Jahn R
Nature communications (2019) 101: 1608. 302 211 WB; tested species: human
The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications.
Götzke H, Kilisch M, Martínez-Carranza M, Sograte-Idrissi S, Rajavel A, Schlichthaerle T, Engels N, Jungmann R, Stenmark P, Opazo F, Frey S, et al.
Nature communications (2019) 101: 4403. 302 211 WB; tested species: mouse
Slowing ribosome velocity restores folding and function of mutant CFTR.
Oliver KE, Rauscher R, Mijnders M, Wang W, Wolpert MJ, Maya J, Sabusap CM, Kesterson RA, Kirk KL, Rab A, Braakman I, et al.
The Journal of clinical investigation (2019) : . 302 211 WB; tested species: human
Increased expression of heme-binding protein 1 early in Alzheimer's disease is linked to neurotoxicity.
Yagensky O, Kohansal-Nodehi M, Gunaseelan S, Rabe T, Zafar S, Zerr I, Härtig W, Urlaub H, Chua JJ
eLife (2019) 8: . 302 211 WB; tested species: mouse
Truncated tau deregulates synaptic markers in rat model for human tauopathy.
Jadhav S, Katina S, Kovac A, Kazmerova Z, Novak M, Zilka N
Frontiers in cellular neuroscience (2015) 9: 24. 302 211 WB
BDNF enhances spontaneous and activity-dependent neurotransmitter release at excitatory terminals but not at inhibitory terminals in hippocampal neurons.
Shinoda Y, Ahmed S, Ramachandran B, Bharat V, Brockelt D, Altas B, Dean C
Frontiers in synaptic neuroscience (2014) 6: 27. 302 211 WB; tested species: rat
Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission.
Spangler SA, Schmitz SK, Kevenaar JT, de Graaff E, de Wit H, Demmers J, Toonen RF, Hoogenraad CC
The Journal of cell biology (2013) 2016: 915-28. 302 211 WB; tested species: rat
Exocyst sec5 regulates exocytosis of newcomer insulin granules underlying biphasic insulin secretion.
Xie L, Zhu D, Kang Y, Liang T, He Y, Gaisano HY
PloS one (2013) 87: e67561. 302 211 WB; tested species: human,mouse,rat
Doc2b is a high-affinity Ca2+ sensor for spontaneous neurotransmitter release.
Groffen AJ, Martens S, Díez Arazola R, Cornelisse LN, Lozovaya N, de Jong AP, Goriounova NA, Habets RL, Takai Y, Borst JG, Brose N, et al.
Science (New York, N.Y.) (2010) 3275973: 1614-8. 302 211 WB; tested species: mouse
Complexins facilitate neurotransmitter release at excitatory and inhibitory synapses in mammalian central nervous system.
Xue M, Stradomska A, Chen H, Brose N, Zhang W, Rosenmund C, Reim K
Proceedings of the National Academy of Sciences of the United States of America (2008) 10522: 7875-80. 302 211 WB; tested species: mouse
Circumvention of common labelling artefacts using secondary nanobodies.
Sograte-Idrissi S, Schlichthaerle T, Duque-Afonso CJ, Alevra M, Strauss S, Moser T, Jungmann R, Rizzoli SO, Opazo F
Nanoscale (2020) 1218: 10226-10239. 302 211 ICC; tested species: monkey
Intrinsic refractive index matched 3D dSTORM with two objectives: Comparison of detection techniques.
Schmidt NC, Kahms M, Hüve J, Klingauf J
Scientific reports (2018) 81: 13343. 302 211 ICC; tested species: mouse
Regulated Dynamic Trafficking of Neurexins Inside and Outside of Synaptic Terminals.
Neupert C, Schneider R, Klatt O, Reissner C, Repetto D, Biermann B, Niesmann K, Missler M, Heine M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3540: 13629-47. 302 211 ICC
Background

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape.
Tubulin itself is a globular protein which consists of two polypeptides, α-tubulin and β-tubulin. α- and β-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase ads a C-terminal tyrosin to monomeric α-tubulin.
Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated α-tubulin is referred to as Glu-α-tubulin. Another post-translational modification of detyrosinated α-tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle. A third variant of detyrosinated α-tubulin is Δ2-tubulin which lacks the C-terminal glutamic acid. It cannot be tyrosinated by tyrosine ligase and is one of the dominant α-tubulin isoforms in neurons.