Synaptic Systems - alpha-Tubulin

Cat. No.: 302 211

Amount: 100 µg

Price: $420.00

Cat. No. 302 211	100 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: yes ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 up to 1 : 1000 gallery ELISA: yes (see remarks)
Clone	3A2
Subtype	IgG1 (κ light chain)
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of human α-Tubulin 4A. (UniProt Id: P68366)
Reactivity	Reacts with: human (P68366), rat, mouse, vertebrates, invertebrates, yeast. Other species not tested yet.
Specificity	Specific for α-tubulin (glu- and tyr-α-tubulin)
Matching control protein/peptide	302-21P
Remarks	ELISA: Suitable as capture antibodies for sandwich-ELISA with cat. no. 302 203 as detector antibody.
Data sheet	302_211.pdf

References for alpha-Tubulin - 302 211

Reduced synaptic depression in human neurons carrying homozygous disease-causing STXBP1 variant L446F.
Öttl M, Toonen RF, Verhage M
Human molecular genetics (2024) : . 302 211 WB; tested species: stem cells

Tomosyn affects dense core vesicle composition but not exocytosis in mammalian neurons.
Subkhangulova A, Gonzalez-Lozano MA, Groffen AJA, van Weering JRT, Smit AB, Toonen RF, Verhage M
eLife (2023) 12: . 302 211 WB; tested species: mouse

Reduced dynamin-1 levels in neurons lacking MUNC18-1.
Lammertse HCA, Moro A, Saarloos I, Toonen RF, Verhage M
Journal of cell science (2022) 13522: . 302 211 WB; tested species: mouse

The molecular chaperone Cysteine String Protein is required for monomeric SNARE proteins to assemble in trans complexes during human sperm acrosomal exocytosis.
Montero KF, Berberián MV, Mayorga LS, Tomes CN, Ruete MC
Biology of reproduction (2022) : . 302 211 WB; tested species: human

Plasticity-Related Gene 5 Is Expressed in a Late Phase of Neurodifferentiation After Neuronal Cell-Fate Determination.
Gross I, Brandt N, Vonk D, Köper F, Wöhlbrand L, Rabus R, Witt M, Heep A, Plösch T, Hipp MS, Bräuer AU, et al.
Frontiers in cellular neuroscience (2022) 16: 797588. 302 211 WB; tested species: mouse

Positive epistasis between disease-causing missense mutations and silent polymorphism with effect on mRNA translation velocity.
Rauscher R, Bampi GB, Guevara-Ferrer M, Santos LA, Joshi D, Mark D, Strug LJ, Rommens JM, Ballmann M, Sorscher EJ, Oliver KE, et al.
Proceedings of the National Academy of Sciences of the United States of America (2021) 1184: . 302 211 WB; tested species: human

Pancreas-specific SNAP23 depletion prevents pancreatitis by attenuating pathological basolateral exocytosis and formation of trypsin-activating autolysosomes.
Dolai S, Takahashi T, Qin T, Liang T, Xie L, Kang F, Miao YF, Xie H, Kang Y, Manuel J, Winter E, et al.
Autophagy (2020) : 1-14. 302 211 WB; tested species: human

Increased expression of heme-binding protein 1 early in Alzheimer's disease is linked to neurotoxicity.
Yagensky O, Kohansal-Nodehi M, Gunaseelan S, Rabe T, Zafar S, Zerr I, Härtig W, Urlaub H, Chua JJ
eLife (2019) 8: . 302 211 WB; tested species: mouse

Slowing ribosome velocity restores folding and function of mutant CFTR.
Oliver KE, Rauscher R, Mijnders M, Wang W, Wolpert MJ, Maya J, Sabusap CM, Kesterson RA, Kirk KL, Rab A, Braakman I, et al.
The Journal of clinical investigation (2019) : . 302 211 WB; tested species: human

The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications.
Götzke H, Kilisch M, Martínez-Carranza M, Sograte-Idrissi S, Rajavel A, Schlichthaerle T, Engels N, Jungmann R, Stenmark P, Opazo F, Frey S, et al.
Nature communications (2019) 101: 4403. 302 211 WB; tested species: mouse

SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.
Koike S, Jahn R
Nature communications (2019) 101: 1608. 302 211 WB; tested species: human

Truncated tau deregulates synaptic markers in rat model for human tauopathy.
Jadhav S, Katina S, Kovac A, Kazmerova Z, Novak M, Zilka N
Frontiers in cellular neuroscience (2015) 9: 24. 302 211 WB

BDNF enhances spontaneous and activity-dependent neurotransmitter release at excitatory terminals but not at inhibitory terminals in hippocampal neurons.
Shinoda Y, Ahmed S, Ramachandran B, Bharat V, Brockelt D, Altas B, Dean C
Frontiers in synaptic neuroscience (2014) 6: 27. 302 211 WB; tested species: rat

Exocyst sec5 regulates exocytosis of newcomer insulin granules underlying biphasic insulin secretion.
Xie L, Zhu D, Kang Y, Liang T, He Y, Gaisano HY
PloS one (2013) 87: e67561. 302 211 WB; tested species: human,mouse,rat

Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission.
Spangler SA, Schmitz SK, Kevenaar JT, de Graaff E, de Wit H, Demmers J, Toonen RF, Hoogenraad CC
The Journal of cell biology (2013) 2016: 915-28. 302 211 WB; tested species: rat

Doc2b is a high-affinity Ca2+ sensor for spontaneous neurotransmitter release.
Groffen AJ, Martens S, Díez Arazola R, Cornelisse LN, Lozovaya N, de Jong AP, Goriounova NA, Habets RL, Takai Y, Borst JG, Brose N, et al.
Science (New York, N.Y.) (2010) 3275973: 1614-8. 302 211 WB; tested species: mouse

Complexins facilitate neurotransmitter release at excitatory and inhibitory synapses in mammalian central nervous system.
Xue M, Stradomska A, Chen H, Brose N, Zhang W, Rosenmund C, Reim K
Proceedings of the National Academy of Sciences of the United States of America (2008) 10522: 7875-80. 302 211 WB; tested species: mouse

Cat. No.: 302 211

Amount: 100 µg

Price: $420.00

ICC

IHC

IHC-P

Background

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape.
Tubulin itself is a globular protein which consists of two polypeptides, α-tubulin and β-tubulin. α- and β-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter.

Assembled microtubules can be detyrosinated by a carboxypeptidaseS called vasohibins / SVBPs. Detyrosinated α-tubulin is referred to as Glu-α-tubulin and occurs for exemple in neurons. This reaction can be reverted by Tubulin tyrosine ligase (TTL) that ads a C-terminal tyrosin to Glu α-tubulin.
Another post-translational modification of α-tubulin is C-terminal polyglutamylation which is also characteristic for microtubules in neuronal cells and the mitotic spindle. A third variant of detyrosinated α-tubulin is Δ2-tubulin which lacks the C-terminal glutamic acid. It cannot be tyrosinated by TTL and is one of the dominant α-tubulin isoforms in neurons.

1 ) Erck C et al., Proc. Natl. Acad. Sci. U.S.A. (2005)
2 ) Contin MA et al., Biochem. J. (1999)
3 ) Paturle-Lafanechère L et al., J. Cell. Sci. (1994)
4 ) Ersfeld K et al., J. Cell Biol. (1993)
5 ) Rüdiger M et al., FEBS Lett. (1992)
6 ) Caron JM et al., J. Cell Biol. (1985)
7 ) Caron JM et al., Nature ()

Protocols

Other products for this target

alpha-Tubulin antibody - 302 211

References for alpha-Tubulin - 302 211